Clinical and epidemiologic evaluation of a 2020 chikungunya outbreak in Cambodia.
Alphavirus
Cambodia
Chikungunya
Outbreak
Southeast Asia
Surveillance
Vector-borne disease
Journal
BMC infectious diseases
ISSN: 1471-2334
Titre abrégé: BMC Infect Dis
Pays: England
ID NLM: 100968551
Informations de publication
Date de publication:
17 Dec 2022
17 Dec 2022
Historique:
received:
28
09
2022
accepted:
09
12
2022
entrez:
16
12
2022
pubmed:
17
12
2022
medline:
21
12
2022
Statut:
epublish
Résumé
In 2020, the Kingdom of Cambodia experienced a nationwide outbreak of chikungunya virus (CHIKV). Despite an increase in the frequency of outbreaks and expanding geographic range of CHIKV, diagnostic challenges remain, and limited surveillance data of sufficient granularity are available to characterize epidemiological profiles and disease dynamics of the virus. An ongoing and long-standing cross-sectional study of acute undifferentiated febrile illness (AUFI) in Cambodia was leveraged to describe the disease epidemiology and characterize the clinical presentation of patients diagnosed with CHIKV during the 2020 outbreak. Participants presenting with AUFI symptoms at ten study locations provided acute and convalescent blood samples and were tested for CHIKV using a reverse transcription-polymerase chain reaction (RT-PCR) and serological diagnostic methods including IgM and IgG. Acute and follow-up clinical data were also collected. From 1194 participant blood samples tested, 331 (27.7%) positive CHIKV cases were detected. Most CHIKV positive individuals (280, 84.6%) reported having a fever 3 to 4 days prior to visiting a health facility. Symptoms including chills, joint pain, nausea, vomiting, and lesions were all statistically significant among CHIKV positive participants compared to CHIKV negative AUFI participants. Cough was negatively associated with CHIKV positive participants. Positivity proportions were significantly higher among adults compared to children. No significant difference was found in positivity proportion between rainy and dry seasons during the outbreak. Positive CHIKV cases were detected in all study site provinces, with the highest test positivity proportion recorded in the rural northeast province of Kratie. Surveillance data captured in this study provided a clinical and epidemiological characterization of positive CHIKV patients presenting at selected health facilities in Cambodia in 2020, and highlighted the widespread distribution of the outbreak, impacting both urban and rural locations. Findings also illustrated the importance of utilizing both RT-PCR and serological testing for effective CHIKV surveillance.
Sections du résumé
BACKGROUND
BACKGROUND
In 2020, the Kingdom of Cambodia experienced a nationwide outbreak of chikungunya virus (CHIKV). Despite an increase in the frequency of outbreaks and expanding geographic range of CHIKV, diagnostic challenges remain, and limited surveillance data of sufficient granularity are available to characterize epidemiological profiles and disease dynamics of the virus.
METHODS
METHODS
An ongoing and long-standing cross-sectional study of acute undifferentiated febrile illness (AUFI) in Cambodia was leveraged to describe the disease epidemiology and characterize the clinical presentation of patients diagnosed with CHIKV during the 2020 outbreak. Participants presenting with AUFI symptoms at ten study locations provided acute and convalescent blood samples and were tested for CHIKV using a reverse transcription-polymerase chain reaction (RT-PCR) and serological diagnostic methods including IgM and IgG. Acute and follow-up clinical data were also collected.
RESULTS
RESULTS
From 1194 participant blood samples tested, 331 (27.7%) positive CHIKV cases were detected. Most CHIKV positive individuals (280, 84.6%) reported having a fever 3 to 4 days prior to visiting a health facility. Symptoms including chills, joint pain, nausea, vomiting, and lesions were all statistically significant among CHIKV positive participants compared to CHIKV negative AUFI participants. Cough was negatively associated with CHIKV positive participants. Positivity proportions were significantly higher among adults compared to children. No significant difference was found in positivity proportion between rainy and dry seasons during the outbreak. Positive CHIKV cases were detected in all study site provinces, with the highest test positivity proportion recorded in the rural northeast province of Kratie.
CONCLUSIONS
CONCLUSIONS
Surveillance data captured in this study provided a clinical and epidemiological characterization of positive CHIKV patients presenting at selected health facilities in Cambodia in 2020, and highlighted the widespread distribution of the outbreak, impacting both urban and rural locations. Findings also illustrated the importance of utilizing both RT-PCR and serological testing for effective CHIKV surveillance.
Identifiants
pubmed: 36526991
doi: 10.1186/s12879-022-07936-9
pii: 10.1186/s12879-022-07936-9
pmc: PMC9758031
doi:
Substances chimiques
Antibodies, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
949Informations de copyright
© 2022. The Author(s).
Références
Biomed Res Int. 2015;2015:834371
pubmed: 26509163
PLoS Negl Trop Dis. 2021 Nov 16;15(11):e0009963
pubmed: 34784371
Lancet Infect Dis. 2007 May;7(5):319-27
pubmed: 17448935
Vector Borne Zoonotic Dis. 2007 Winter;7(4):467-77
pubmed: 18020965
Southeast Asian J Trop Med Public Health. 2004 Jun;35(2):408-15
pubmed: 15691147
Trans R Soc Trop Med Hyg. 2015 Dec;109(12):793-802
pubmed: 26626342
Indian J Med Res. 2009 Mar;129(3):311-5
pubmed: 19491425
J Assoc Physicians India. 2006 Sep;54:725-6
pubmed: 17212022
N Engl J Med. 2015 Mar 26;372(13):1231-9
pubmed: 25806915
Open Rheumatol J. 2016 Nov 30;10:129-140
pubmed: 28077980
BMC Infect Dis. 2016 Mar 03;16:84
pubmed: 26936191
Am J Trop Med Hyg. 2012 Feb;86(2):246-253
pubmed: 22302857
Lancet. 2007 Dec 1;370(9602):1840-6
pubmed: 18061059
Travel Med Infect Dis. 2017 Jan - Feb;15:3-4
pubmed: 28189872
J Biomed Sci. 2021 Dec 2;28(1):84
pubmed: 34857000
Microorganisms. 2022 Feb 03;10(2):
pubmed: 35208808
PLoS Negl Trop Dis. 2022 Jan 12;16(1):e0010069
pubmed: 35020717
Emerg Infect Dis. 2012 Dec;18(12):2066-9
pubmed: 23171736
Viruses. 2017 Dec 01;9(12):
pubmed: 29194359
Viruses. 2022 May 05;14(5):
pubmed: 35632709
Emerg Infect Dis. 2015 Apr;21(4):557-61
pubmed: 25816211
Emerg Microbes Infect. 2019;8(1):70-79
pubmed: 30866761
Am J Trop Med Hyg. 2008 Feb;78(2):333-7
pubmed: 18256441
Trans R Soc Trop Med Hyg. 2010 Jun;104(6):392-9
pubmed: 20171708
Euro Surveill. 2020 Jan;25(1):
pubmed: 31937394
J Virol Methods. 2005 Mar;124(1-2):65-71
pubmed: 15664052
PLoS Negl Trop Dis. 2021 Dec 1;15(12):e0009961
pubmed: 34851949
PLoS Negl Trop Dis. 2020 Jun 1;14(6):e0008355
pubmed: 32479497
Pediatr Infect Dis J. 2015 Jul;34(7):789-91
pubmed: 26069950
Popul Health Metr. 2011 Jan 10;9(1):1
pubmed: 21219615
Clin Microbiol Infect. 2010 Dec;16(12):1702-4
pubmed: 21040155
BMC Infect Dis. 2016 Apr 26;16:183
pubmed: 27112553
Euro Surveill. 2015 Apr 30;20(17):
pubmed: 25955774
PLoS Negl Trop Dis. 2014 Sep 11;8(9):e3120
pubmed: 25210729
Int J Infect Dis. 2017 May;58:69-76
pubmed: 28288924
Diagnostics (Basel). 2021 Apr 30;11(5):
pubmed: 33946597
J Gen Virol. 2007 Sep;88(Pt 9):2363-2377
pubmed: 17698645
PLoS One. 2021 Mar 10;16(3):e0247314
pubmed: 33690657
J Hist Med Allied Sci. 1971 Jul;26(3):243-62
pubmed: 4938938