Histological evaluation of PAXgene tissue fixation in Barrett's esophagus and esophageal adenocarcinoma diagnostics.
Dysplasia
Esophageal adenocarcinoma
PAXgene-fixed paraffin-embedded
Journal
Virchows Archiv : an international journal of pathology
ISSN: 1432-2307
Titre abrégé: Virchows Arch
Pays: Germany
ID NLM: 9423843
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
received:
16
08
2022
accepted:
30
11
2022
revised:
01
11
2022
medline:
5
5
2023
pubmed:
18
12
2022
entrez:
17
12
2022
Statut:
ppublish
Résumé
The dysplasia grading of Barrett's esophagus (BE), based on the histomorphological assessment of formalin-fixed, paraffin-embedded (FFPE) tissue, suffers from high interobserver variability leading to an unsatisfactory prediction of cancer risk. Thus, pre-analytic preservation of biological molecules, which could improve risk prediction in BE enabling molecular and genetic analysis, is needed. We aimed to evaluate such a molecular pre-analytic fixation tool, PAXgene-fixed paraffin-embedded (PFPE) biopsies, and their suitability for histomorphological BE diagnostics in comparison to FFPE. In a ring trial, 9 GI pathologists evaluated 116 digital BE slides of non-dysplastic BE (NDBE), low-grade dysplasia (LGD), high-grade dysplasia (HGD), and esophageal adenocarcinomas (EAC) using virtual microscopy. Overall quality, cytological and histomorphological parameters, dysplasia criteria, and diagnosis were analyzed. PFPE showed better preservation of nuclear details as chromatin and nucleoli, whereas overall quality and histomorphologic parameters as visibility of basal lamina, goblet cells, and presence of artifacts were scored as equal to FFPE. The interobserver reproducibility with regard to the diagnosis was best for NDBE and EAC (κ
Identifiants
pubmed: 36527466
doi: 10.1007/s00428-022-03471-9
pii: 10.1007/s00428-022-03471-9
pmc: PMC10156762
doi:
Types de publication
Clinical Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
887-898Subventions
Organisme : Deutsche Krebshilfe
ID : 108296
Organisme : Bundesministerium für Bildung und Forschung
ID : 01EX1221B
Organisme : Bundesministerium für Bildung und Forschung
ID : PM25
Informations de copyright
© 2022. The Author(s).
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