Monitoring α-synuclein aggregation.
Amyloid
Fibril
Fluorescence
Oligomerization
Parkinson's disease
Polymorphism
Structural techniques
Synucleinopathy
α-Synuclein
Journal
Neurobiology of disease
ISSN: 1095-953X
Titre abrégé: Neurobiol Dis
Pays: United States
ID NLM: 9500169
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
10
11
2022
revised:
12
12
2022
accepted:
14
12
2022
pubmed:
18
12
2022
medline:
28
12
2022
entrez:
17
12
2022
Statut:
ppublish
Résumé
Synucleinopathies, including Parkinson's disease (PD), dementia with Lewy Bodies (DLB), and multiple system atrophy (MSA), are characterized by the misfolding and subsequent aggregation of alpha-synuclein (α-syn) that accumulates in cytoplasmic inclusions bodies in the cells of affected brain regions. Since the seminal report of likely-aggregated α-syn presence within the Lewy bodies by Spillantini et al. in 1997, the keyword "synuclein aggregation" has appeared in over 6000 papers (Source: PubMed October 2022). Studying, observing, describing, and quantifying α-syn aggregation is therefore of paramount importance, whether it happens in tubo, in vitro, in post-mortem samples, or in vivo. The past few years have witnessed tremendous progress in understanding aggregation mechanisms and identifying various polymorphs. In this context of growing complexity, it is of utmost importance to understand what tools we possess, what exact information they provide, and in what context they may be applied. Nonetheless, it is also crucial to rationalize the relevance of the information and the limitations of these methods for gauging the final result. In this review, we present the main techniques that have shaped the current views about α-syn structure and dynamics, with particular emphasis on the recent breakthroughs that may change our understanding of synucleinopathies.
Identifiants
pubmed: 36527982
pii: S0969-9961(22)00358-8
doi: 10.1016/j.nbd.2022.105966
pmc: PMC9875312
pii:
doi:
Substances chimiques
alpha-Synuclein
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105966Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest EB is a director and shareholder of Motac Neuroscience Ltd. The other authors declare no conflict of interest.
Références
Acta Neuropathol Commun. 2013 May 09;1:6
pubmed: 24252244
ACS Nano. 2020 May 26;14(5):5213-5222
pubmed: 32159944
EMBO J. 2004 May 19;23(10):2039-46
pubmed: 15103328
Science. 2003 Dec 5;302(5651):1772-5
pubmed: 14657500
J Mol Biol. 2012 Jun 29;420(1-2):99-111
pubmed: 22516611
Nat Cell Biol. 2002 Feb;4(2):160-4
pubmed: 11813001
Proc Natl Acad Sci U S A. 2020 Mar 3;117(9):4971-4982
pubmed: 32075919
Brain. 2019 Nov 1;142(11):3565-3579
pubmed: 31580415
Nat Rev Methods Primers. 2021;1:
pubmed: 34368784
Sci Adv. 2020 Oct 2;6(40):
pubmed: 33008896
Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4194-9
pubmed: 21325059
Brain. 2020 Mar 1;143(3):920-931
pubmed: 32025699
J Neurosci Methods. 2016 Jun 15;266:141-50
pubmed: 27039974
Commun Biol. 2021 May 21;4(1):613
pubmed: 34021258
Elife. 2018 Jul 03;7:
pubmed: 29969391
Proc Natl Acad Sci U S A. 2018 May 22;115(21):E4758-E4766
pubmed: 29735687
J Neurosci Res. 2021 Oct;99(10):2525-2539
pubmed: 34292621
J Biol Chem. 2003 Apr 25;278(17):15313-8
pubmed: 12586824
J Neurochem. 2016 Oct;139 Suppl 1:240-255
pubmed: 26190401
Biomolecules. 2022 Jan 28;12(2):
pubmed: 35204729
Nat Rev Chem. 2021 Apr;5(4):277-294
pubmed: 37117282
Nat Chem Biol. 2015 Jun;11(6):373-7
pubmed: 25978986
Chemistry. 2018 May 17;24(28):7210-7216
pubmed: 29543355
Front Aging Neurosci. 2022 Mar 10;14:853050
pubmed: 35360213
Biochemistry. 2002 Aug 13;41(32):10209-17
pubmed: 12162735
Biochim Biophys Acta. 2010 Jul;1804(7):1405-12
pubmed: 20399286
Nat Commun. 2021 Apr 14;12(1):2110
pubmed: 33854052
Neuropathol Appl Neurobiol. 2017 Dec;43(7):604-620
pubmed: 28386933
Chem Soc Rev. 2021 Feb 1;50(2):1219-1250
pubmed: 33284303
EBioMedicine. 2018 Mar;29:13-22
pubmed: 29433982
Chem Soc Rev. 2018 Nov 26;47(23):8842-8880
pubmed: 30361725
Neurobiol Dis. 2019 Sep;129:38-43
pubmed: 31078683
FASEB J. 2006 Mar;20(3):419-25
pubmed: 16507759
ACS Nano. 2020 Aug 25;14(8):9979-9989
pubmed: 32678577
Nat Methods. 2006 Dec;3(12):995-1000
pubmed: 17072308
Exp Mol Med. 2017 Apr 7;49(4):e314
pubmed: 28386127
Science. 2012 Nov 16;338(6109):949-53
pubmed: 23161999
Neurobiol Dis. 2020 Jul;141:104876
pubmed: 32339655
PLoS One. 2008 Apr 02;3(4):e1867
pubmed: 18382657
Biochemistry. 2015 Jan 20;54(2):279-92
pubmed: 25490121
NPJ Parkinsons Dis. 2021 Dec 20;7(1):119
pubmed: 34930911
Methods Mol Biol. 2019;1948:221-233
pubmed: 30771181
Exp Neurol. 2021 Oct;344:113810
pubmed: 34270920
Ann Neurol. 2014 Mar;75(3):351-62
pubmed: 24243558
Neurotherapeutics. 2021 Jul;18(3):1692-1709
pubmed: 34258749
Neurobiol Dis. 2015 Jul;79:81-99
pubmed: 25937088
Sci Rep. 2017 Oct 2;7(1):12433
pubmed: 28970487
ACS Chem Biol. 2020 Mar 20;15(3):640-645
pubmed: 32065743
PLoS One. 2011;6(10):e25954
pubmed: 22016789
Biochim Biophys Acta. 2016 Oct;1864(10):1419-27
pubmed: 27475048
Eur Biophys J. 2018 May;47(4):345-362
pubmed: 29255947
J Neurochem. 2021 May;157(4):872-888
pubmed: 32772367
Sci Adv. 2020 Nov 11;6(46):
pubmed: 33177086
Methods Mol Biol. 2005;299:129-51
pubmed: 15980599
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8637-42
pubmed: 18550842
Elife. 2019 Dec 09;8:
pubmed: 31815671
J Mol Biol. 1999 Jan 8;285(1):33-9
pubmed: 9878384
R Soc Open Sci. 2017 Jan 4;4(1):160696
pubmed: 28280572
Biomedicines. 2022 Sep 02;10(9):
pubmed: 36140270
Cell Rep. 2019 Nov 26;29(9):2862-2874.e9
pubmed: 31775051
Neuropathology. 2006 Aug;26(4):338-45
pubmed: 16961071
ACS Nano. 2019 Mar 26;13(3):3232-3242
pubmed: 30811930
Neuropathol Appl Neurobiol. 2018 Dec;44(7):722-736
pubmed: 29676021
FEBS J. 2023 Feb;290(3):554-583
pubmed: 34862849
Nat Methods. 2007 Dec;4(12):1023-30
pubmed: 18026110
Proc Natl Acad Sci U S A. 1998 May 26;95(11):6469-73
pubmed: 9600990
Am J Pathol. 1999 Oct;155(4):1241-51
pubmed: 10514406
Biophys Chem. 2016 Mar;210:14-26
pubmed: 26234527
Nat Commun. 2018 Aug 27;9(1):3465
pubmed: 30150626
Trends Biochem Sci. 2007 Sep;32(9):407-14
pubmed: 17764955
Prion. 2011 Jul-Sep;5(3):150-3
pubmed: 21778820
ACS Chem Neurosci. 2021 Apr 21;12(8):1293-1298
pubmed: 33819025
Elife. 2013 Nov 19;2:e01345
pubmed: 24252878
Exp Neurol. 2002 Sep;177(1):88-94
pubmed: 12429213
Brain Res Rev. 2007 Jan;53(1):135-60
pubmed: 16959325
J Biol Chem. 2017 Jun 2;292(22):9034-9050
pubmed: 28373279
Sci Rep. 2020 Jul 30;10(1):12827
pubmed: 32732936
Eur J Neurosci. 2004 Feb;19(4):845-54
pubmed: 15009131
Nature. 2016 Feb 4;530(7588):45-50
pubmed: 26808899
Cell Rep. 2019 Apr 16;27(3):916-927.e5
pubmed: 30995486
Nature. 1997 Aug 28;388(6645):839-40
pubmed: 9278044
Am J Pathol. 1999 Oct;155(4):1173-81
pubmed: 10514400
Bioorg Med Chem Lett. 2014 Jul 15;24(14):3194-8
pubmed: 24835632
ACS Chem Neurosci. 2020 Feb 5;11(3):233-241
pubmed: 31894960
PLoS One. 2010 May 11;5(5):e10589
pubmed: 20485674
Protein Sci. 1993 Mar;2(3):404-10
pubmed: 8453378
Curr Opin Chem Biol. 2021 Oct;64:57-66
pubmed: 34091264
J Neuroinflammation. 2018 May 1;15(1):129
pubmed: 29716614
Org Biomol Chem. 2016 Feb 7;14(5):1584-92
pubmed: 26695131
FEBS Open Bio. 2021 Apr;11(4):999-1013
pubmed: 33548114
Neurobiol Dis. 2021 May;152:105291
pubmed: 33556542
Biophys Rev. 2016;8(4):429-439
pubmed: 28003858
Science. 2000 Nov 3;290(5493):985-9
pubmed: 11062131
Elife. 2019 Feb 05;8:
pubmed: 30720432
Protein Sci. 2004 Dec;13(12):3314-21
pubmed: 15537750
J Mol Biol. 1997 Oct 31;273(3):729-39
pubmed: 9356260
Sci Rep. 2015 Dec 01;5:17625
pubmed: 26621077
Nature. 2022 Oct;610(7933):791-795
pubmed: 36108674
Acta Neuropathol Commun. 2022 Sep 6;10(1):133
pubmed: 36068646
Cell Chem Biol. 2018 Jun 21;25(6):797-801.e4
pubmed: 29681525
PLoS One. 2011;6(8):e23338
pubmed: 21858077
Front Neurol. 2018 Mar 22;9:180
pubmed: 29623065
Med Sci (Paris). 2022 Jan;38(1):45-51
pubmed: 35060886
Brain. 2022 Apr 29;145(3):1001-1017
pubmed: 35285474
PLoS One. 2014 Mar 11;9(3):e90857
pubmed: 24618582
Nat Commun. 2022 Sep 20;13(1):5512
pubmed: 36127374
NPJ Parkinsons Dis. 2022 Jun 2;8(1):68
pubmed: 35655068
Nat Commun. 2013;4:2575
pubmed: 24108358
Nat Struct Mol Biol. 2016 May;23(5):409-15
pubmed: 27018801
Prion. 2020 Dec;14(1):11-19
pubmed: 31876447
Acta Neuropathol Commun. 2016 Dec 9;4(1):128
pubmed: 27938414
Chem Biol Drug Des. 2011 Jun;77(6):399-411
pubmed: 21457473
Nat Commun. 2014 Aug 26;5:4755
pubmed: 25156829
Cells. 2020 Oct 29;9(11):
pubmed: 33138150
Autophagy. 2016 Oct 2;12(10):1849-1863
pubmed: 27485532
Nat Commun. 2018 Sep 6;9(1):3609
pubmed: 30190461
Biophys J. 2020 Mar 24;118(6):1301-1320
pubmed: 32059758
Essays Biochem. 2014;56:11-39
pubmed: 25131584
Life (Basel). 2020 Aug 11;10(8):
pubmed: 32796544
Brain. 2009 Apr;132(Pt 4):1093-101
pubmed: 19155272
Brain. 2022 Apr 18;145(2):584-595
pubmed: 34894214
Sci Rep. 2017 Aug 8;7(1):7506
pubmed: 28790319
J Neurochem. 2019 Sep;150(5):522-534
pubmed: 31254394
Prog Neurobiol. 2017 Aug;155:171-193
pubmed: 26209472
Nat Cell Biol. 2000 Jul;2(7):E115-9
pubmed: 10878819
Nat Methods. 2007 Apr;4(4):345-51
pubmed: 17351621
Biochemistry. 2018 Oct 16;57(41):5989-5995
pubmed: 30239196
J Mol Biol. 2013 May 27;425(10):1765-81
pubmed: 23415897
Hum Mol Genet. 2014 Sep 1;23(17):4491-509
pubmed: 24728187
Nature. 2015 Jun 18;522(7556):340-4
pubmed: 26061766
Brain. 2015 Jun;138(Pt 6):1642-57
pubmed: 25732184
Nat Commun. 2020 Sep 28;11(1):4885
pubmed: 32985503
Nat Neurosci. 2019 Jul;22(7):1099-1109
pubmed: 31235907
Proc Natl Acad Sci U S A. 2009 Nov 24;106(47):20051-6
pubmed: 19892735
Nat Rev Mol Cell Biol. 2018 Dec;19(12):755-773
pubmed: 30237470
J Neurochem. 2019 Sep;150(5):612-625
pubmed: 31055836
Chem Sci. 2020 Feb 21;11(18):4578-4583
pubmed: 34122915
J Struct Biol. 2007 May;158(2):244-53
pubmed: 17502240
Nature. 2013 Jun 13;498(7453):E4-6; discussion E6-7
pubmed: 23765500
FASEB J. 2011 Jan;25(1):326-36
pubmed: 20876215
FASEB J. 2019 Feb;33(2):2116-2131
pubmed: 30252534
J Mol Biol. 2010 Jan 22;395(3):627-42
pubmed: 19891973
Methods Enzymol. 1999;309:274-84
pubmed: 10507030
Proc Natl Acad Sci U S A. 2020 Feb 18;117(7):3592-3602
pubmed: 32015135
J Mol Biol. 2001 Apr 6;307(4):1061-73
pubmed: 11286556
Nat Neurosci. 2019 Jan;22(1):65-77
pubmed: 30559480
Biochim Biophys Acta. 2013 Oct;1828(10):2328-38
pubmed: 23746423
J Biol Chem. 2019 Jan 18;294(3):1045-1058
pubmed: 30478174
J Neurochem. 2016 Oct;139 Suppl 1:121-130
pubmed: 27091001
J Struct Biol. 2011 Jan;173(1):1-13
pubmed: 20868754
Ann Clin Transl Neurol. 2016 Aug 28;3(10):812-818
pubmed: 27752516
Sci Adv. 2020 May 13;6(20):eaaz9165
pubmed: 32426502
Proc Natl Acad Sci U S A. 2000 Jan 18;97(2):571-6
pubmed: 10639120
PLoS One. 2019 Mar 22;14(3):e0213663
pubmed: 30901378
Vaccines (Basel). 2022 Aug 30;10(9):
pubmed: 36146508
Acta Neuropathol Commun. 2020 Aug 28;8(1):150
pubmed: 32859276
Life (Basel). 2021 May 11;11(5):
pubmed: 34064766
Nat Struct Mol Biol. 2019 Nov;26(11):1044-1052
pubmed: 31695184
Chemphyschem. 2011 Feb 25;12(3):673-680
pubmed: 21308945
Nature. 2015 Sep 24;525(7570):486-90
pubmed: 26352473
Nat Commun. 2019 Dec 4;10(1):5535
pubmed: 31797870
J Neurochem. 2008 Jul;106(2):529-40
pubmed: 18410502
Anal Chem. 2017 Dec 19;89(24):13044-13048
pubmed: 29172450
J Neurochem. 2022 Jun;161(6):506-515
pubmed: 35234288
Nat Commun. 2020 May 26;11(1):2643
pubmed: 32457390
J Biol Chem. 2021 Jan-Jun;296:100737
pubmed: 33933456
J Chem Phys. 2018 Mar 28;148(12):123322
pubmed: 29604892
Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):11282-6
pubmed: 8248242
Int J Mol Sci. 2021 Aug 25;22(17):
pubmed: 34502063
NPJ Parkinsons Dis. 2022 Jan 13;8(1):10
pubmed: 35027576
Trends Biochem Sci. 2010 Nov;35(11):643-51
pubmed: 20561791
Rev Neurol (Paris). 2019 Sep - Oct;175(7-8):458-463
pubmed: 31296398
J Neurosci. 2008 Nov 19;28(47):12305-17
pubmed: 19020024
Nature. 2018 May;557(7706):558-563
pubmed: 29743672
Nature. 2011 Aug 14;477(7362):107-10
pubmed: 21841800
Mov Disord. 2020 Feb;35(2):268-278
pubmed: 31758740
J Biol Chem. 2017 Oct 13;292(41):16891-16903
pubmed: 28760825
Methods Mol Biol. 2019;1948:123-143
pubmed: 30771175
Acta Neuropathol. 2019 Mar;137(3):455-466
pubmed: 30721406
Anal Chem. 2016 Jan 5;88(1):718-24
pubmed: 26637393
Cell Rep. 2015 Mar 3;10(8):1252-60
pubmed: 25732816
Neuropathology. 2007 Oct;27(5):494-506
pubmed: 18018486
Nature. 2020 Feb;578(7794):273-277
pubmed: 32025029
Biochemistry. 1996 Oct 29;35(43):13709-15
pubmed: 8901511
Methods Mol Biol. 2012;849:121-35
pubmed: 22528087
J Neurol Sci. 1992 Feb;107(2):172-82
pubmed: 1314292
Am J Pathol. 1998 Apr;152(4):879-84
pubmed: 9546347
Neurobiol Dis. 2020 Dec;146:105086
pubmed: 32971232
J Am Chem Soc. 2019 Feb 6;141(5):1893-1897
pubmed: 30657670
Proc Natl Acad Sci U S A. 2005 Nov 1;102(44):15871-6
pubmed: 16247008
J Biol Chem. 2000 Nov 3;275(44):34574-9
pubmed: 10942772
J Biol Chem. 1998 Apr 17;273(16):9443-9
pubmed: 9545270
Nat Neurosci. 2020 Jan;23(1):21-31
pubmed: 31792467
Biochim Biophys Acta. 2005 Aug 10;1751(2):119-39
pubmed: 16027053
PLoS One. 2018 Apr 26;13(4):e0196056
pubmed: 29698510
Cell Res. 2018 Sep;28(9):897-903
pubmed: 30065316
Nature. 2020 Sep;585(7825):464-469
pubmed: 32461689
Cell Res. 2020 Apr;30(4):360-362
pubmed: 32203130
J Biol Chem. 2021 Jan-Jun;296:100508
pubmed: 33675750
Biosci Rep. 2019 Jan 15;39(1):
pubmed: 30567726
J Mol Biol. 2008 Oct 31;383(1):214-23
pubmed: 18775438
Neuroscience. 2001;104(3):901-12
pubmed: 11440819
J Am Chem Soc. 2010 Jun 23;132(24):8407-18
pubmed: 20499903
Trends Biochem Sci. 2017 Oct;42(10):777-787
pubmed: 28916413
Neurochem Int. 2022 Jul;157:105345
pubmed: 35500664
J Mol Biol. 2009 Jul 24;390(4):775-90
pubmed: 19481095
Science. 2014 Mar 28;343(6178):1443-4
pubmed: 24675944
J Biol Chem. 2019 Jul 5;294(27):10392-10406
pubmed: 31142553
ACS Nano. 2018 Dec 26;12(12):12247-12254
pubmed: 30525434
PLoS Genet. 2016 Apr 28;12(4):e1005995
pubmed: 27123591
Neuron. 2011 Oct 6;72(1):57-71
pubmed: 21982369
Amyloid. 2018 Sep;25(3):189-196
pubmed: 30486688
Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4897-902
pubmed: 10781096
J Parkinsons Dis. 2023;13(2):255-270
pubmed: 36847016
Ann Neurol. 2002 Aug;52(2):205-10
pubmed: 12210791
Sci Rep. 2019 Feb 27;9(1):2937
pubmed: 30814575