Endothelin-1, over-expressed in SOD1
ET-1
ET-A
ET-B
NSC34-hSOD1G93A cells
TgSOD1-G93A
amyotrophic lateral sclerosis
bioinfomatic analysis
proteomics
Journal
Frontiers in cellular neuroscience
ISSN: 1662-5102
Titre abrégé: Front Cell Neurosci
Pays: Switzerland
ID NLM: 101477935
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
10
2022
accepted:
15
11
2022
entrez:
19
12
2022
pubmed:
20
12
2022
medline:
20
12
2022
Statut:
epublish
Résumé
Endothelin-1 (ET-1), a secreted signaling peptide, is suggested to be involved in multiple actions in various tissues including the brain, but its role in amyotrophic lateral sclerosis (ALS) remains unknown. In this study, we detected the expression changes as well as the cellular localization of ET-1, endothelin A (ET-A) and endothelin B (ET-B) receptors in spinal cord of transgenic SOD1-G93A (TgSOD1-G93A) mice, which showed that the two ET receptors (ET-Rs) expressed mainly on neurons and decreased as the disease progressed especially ET-B, while ET-1 expression was up-regulated and primarily localized on astrocytes. We then explored the possible mechanisms underlying the effect of ET-1 on cultured NSC34-hSOD1G93A cell model. ET-1 showed toxic effect on motor neurons (MNs), which can be rescued by the selective ET-A receptor antagonist BQ-123 or ET-B receptor antagonist BQ-788, suggesting that clinically used ET-Rs pan-antagonist could be a potential strategy for ALS. Using proteomic analysis, we revealed that 110 proteins were differentially expressed in NSC34-hSOD1G93A cells after ET-1 treatment, of which 54 were up-regulated and 56 were down-regulated. Bioinformatic analysis showed that the differentially expressed proteins (DEPs) were primarily enriched in hippo signaling pathway-multiple species, ABC transporters, ErbB signaling pathway and so on. These results provide further insights on the potential roles of ET-1 in ALS and present a new promising therapeutic target to protect MNs of ALS.
Identifiants
pubmed: 36531135
doi: 10.3389/fncel.2022.1069617
pmc: PMC9752095
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1069617Informations de copyright
Copyright © 2022 Zhang, Chen, Li, Li, Wu and Guo.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Autophagy. 2021 Dec;17(12):4029-4042
pubmed: 33749521
Neuroscience. 2014 Dec 5;281:88-98
pubmed: 25270903
Nihon Rinsho. 1993 Jun;51(6):1530-9
pubmed: 8320832
Eur J Neurol. 2020 Oct;27(10):1918-1929
pubmed: 32526057
J Alzheimers Dis. 2012;29(4):853-61
pubmed: 22330820
Cell Rep. 2015 Dec 15;13(10):2090-7
pubmed: 26628380
Exp Neurol. 2019 Jul;317:10-21
pubmed: 30797827
Neurobiol Dis. 2014 May;65:160-71
pubmed: 24423643
Clin Cancer Res. 2018 Jan 1;24(1):14-21
pubmed: 28696243
Neurotox Res. 2017 Jul;32(1):58-70
pubmed: 28285347
Amyotroph Lateral Scler. 2007 Aug;8(4):217-23
pubmed: 17653919
J Proteome Res. 2019 May 3;18(5):2100-2108
pubmed: 30860844
Biotechnol Lett. 2008 Apr;30(4):603-10
pubmed: 18004513
CNS Neurol Disord Drug Targets. 2011 Mar;10(2):147-8
pubmed: 21222630
J Biol Chem. 2019 Aug 16;294(33):12495-12506
pubmed: 31248984
Hum Mol Genet. 2021 Feb 25;29(24):3935-3944
pubmed: 33410474
Skelet Muscle. 2016 Jun 23;6:24
pubmed: 27340545
Int J Clin Exp Pathol. 2009;2(4):390-8
pubmed: 19158936
J Proteomics. 2019 Apr 30;198:36-44
pubmed: 30553948
Mol Neurobiol. 2015;51(3):864-77
pubmed: 24848512
Biomol Concepts. 2013 Aug;4(4):335-47
pubmed: 25436584
Prog Neurobiol. 1996 Oct;50(2-3):83-107
pubmed: 8971979
J Proteome Res. 2018 Apr 6;17(4):1436-1451
pubmed: 29564889
Neurosci Res. 2018 Jan;126:31-38
pubmed: 29054467
J Alzheimers Dis. 2020;74(2):535-544
pubmed: 32065797
Aging Cell. 2021 Sep;20(9):e13465
pubmed: 34415667
Neural Regen Res. 2021 Apr;16(4):643-652
pubmed: 33063715
Science. 1994 Jun 17;264(5166):1772-5
pubmed: 8209258
Anat Rec (Hoboken). 2013 Mar;296(3):378-81
pubmed: 23382140
Cell Mol Neurobiol. 2008 Dec;28(8):1129-38
pubmed: 18683040
Cell Death Discov. 2022 Apr 16;8(1):207
pubmed: 35429992
Life Sci Alliance. 2022 Jul 1;5(11):
pubmed: 35777956
Biology (Basel). 2022 May 16;11(5):
pubmed: 35625487
Science. 2019 Apr 5;364(6435):89-93
pubmed: 30948552
Neurol Res. 2003 Mar;25(2):195-200
pubmed: 12635522
Biomed Res Int. 2022 Aug 9;2022:3543948
pubmed: 35983249
Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):5654-8
pubmed: 23509249
Sci Rep. 2017 Jan 09;7:40155
pubmed: 28065942
Orphanet J Rare Dis. 2016 Nov 5;11(1):148
pubmed: 27814735
Cell Death Dis. 2021 Apr 12;12(4):392
pubmed: 33846297
Prog Neurobiol. 2016 Sep;144:88-102
pubmed: 27132521
J Cereb Blood Flow Metab. 2015 Oct;35(10):1687-96
pubmed: 26104290
Glia. 2003 Jan 15;41(2):180-90
pubmed: 12509808
Neurobiol Aging. 2008 Oct;29(10):1474-84
pubmed: 17448572
Neurochem Int. 2013 Jul;63(1):25-34
pubmed: 23619396
Cell Signal. 2010 Nov;22(11):1615-25
pubmed: 20466059
Neuron. 2014 Mar 19;81(6):1442
pubmed: 28898632
J Biol Chem. 2019 Mar 15;294(11):3920-3933
pubmed: 30670587
Cell. 2003 Aug 22;114(4):445-56
pubmed: 12941273
Int J Mol Sci. 2022 Mar 15;23(6):
pubmed: 35328566
Free Neuropathol. 2021;2:
pubmed: 34977908
World J Diabetes. 2022 Jun 15;13(6):434-441
pubmed: 35800412
Cell Cycle. 2015;14(4):473-80
pubmed: 25590164
Cell Mol Life Sci. 2022 Jul 11;79(8):415
pubmed: 35821142
Neurol Res. 2019 Aug;41(8):749-761
pubmed: 31038018
Nat Commun. 2016 Jul 14;7:11935
pubmed: 27411738
Int J Infect Dis. 2015 May;34:105-11
pubmed: 25820093
Neuroreport. 2003 May 23;14(7):1051-4
pubmed: 12802201
J Neurosci. 2009 Jan 28;29(4):1093-104
pubmed: 19176818
Neuroscience. 2017 Dec 4;365:192-205
pubmed: 29024785
Neurobiol Dis. 2020 Jul;140:104837
pubmed: 32199908
Dev Dyn. 1992 Jul;194(3):209-21
pubmed: 1467557
J Proteomics. 2021 Jan 16;231:103995
pubmed: 33011346
Neurol Res. 2011 Mar;33(2):208-13
pubmed: 21801597