Efficient Generation of Genome-wide Libraries for Protein-ligand Screens Using Gibson Assembly.
Genome-wide library
Genomics
Gibson assembly
High-throughput screen
Nanopore sequencing
Protozoan
Trypanosoma
Yeast surface display
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
20 Nov 2022
20 Nov 2022
Historique:
received:
15
07
2022
revised:
24
08
2022
accepted:
23
09
2022
entrez:
19
12
2022
pubmed:
20
12
2022
medline:
20
12
2022
Statut:
epublish
Résumé
Genome-wide screens using yeast or phage displays are powerful tools for identifying protein-ligand interactions, including drug or vaccine targets, ligand receptors, or protein-protein interactions. However, assembling libraries for genome-wide screens can be challenging and often requires unbiased cloning of 10
Identifiants
pubmed: 36532687
doi: 10.21769/BioProtoc.4558
pii: e4558
pmc: PMC9724014
pii:
doi:
Types de publication
Journal Article
Langues
eng
Informations de copyright
Copyright © 2022 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interests The authors declare that they have no conflicts of interest with the contents of this article.
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