A pioneer factor locally opens compacted chromatin to enable targeted ATP-dependent nucleosome remodeling.
Journal
Nature structural & molecular biology
ISSN: 1545-9985
Titre abrégé: Nat Struct Mol Biol
Pays: United States
ID NLM: 101186374
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
15
04
2022
accepted:
03
11
2022
pubmed:
20
12
2022
medline:
24
1
2023
entrez:
19
12
2022
Statut:
ppublish
Résumé
To determine how different pioneer transcription factors form a targeted, accessible nucleosome within compacted chromatin and collaborate with an ATP-dependent chromatin remodeler, we generated nucleosome arrays in vitro with a central nucleosome containing binding sites for the hematopoietic E-Twenty Six (ETS) factor PU.1 and Basic Leucine Zipper (bZIP) factors C/EBPα and C/EBPβ. Our long-read sequencing reveals that each factor can expose a targeted nucleosome on linker histone-compacted arrays, but with different nuclease sensitivity patterns. The DNA binding domain of PU.1 binds mononucleosomes, but requires an additional intrinsically disordered domain to bind and open compacted chromatin. The canonical mammalian SWI/SNF (cBAF) remodeler was unable to act upon two forms of locally open chromatin unless cBAF was enabled by a separate transactivation domain of PU.1. cBAF potentiates the PU.1 DNA binding domain to weakly open chromatin in the absence of the PU.1 disordered domain. Our findings reveal a hierarchy by which chromatin is opened and show that pioneer factors can provide specificity for action by nucleosome remodelers.
Identifiants
pubmed: 36536103
doi: 10.1038/s41594-022-00886-5
pii: 10.1038/s41594-022-00886-5
pmc: PMC10004348
mid: NIHMS1873033
doi:
Substances chimiques
Nucleosomes
0
Chromatin
0
Transcription Factors
0
DNA
9007-49-2
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
31-37Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM036477
Pays : United States
Organisme : NIDDK NIH HHS
ID : T32 DK007780
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature America, Inc.
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