Micropyramid-patterned, oxygen-permeable bottomed dish for high density culture of pancreatic islets.
3D spheroid
high seeding density culture
micropatterned dish
pancreatic islets
spheroid culture
Journal
Biofabrication
ISSN: 1758-5090
Titre abrégé: Biofabrication
Pays: England
ID NLM: 101521964
Informations de publication
Date de publication:
20 12 2022
20 12 2022
Historique:
received:
25
06
2022
accepted:
30
11
2022
entrez:
20
12
2022
pubmed:
21
12
2022
medline:
22
12
2022
Statut:
epublish
Résumé
The need for maintaining cell-spheroid viability and function within high-density cultures is unmet for various clinical and experimental applications, including cell therapies. One immediate application is for transplantation of pancreatic islets, a clinically recognized treatment option to cure type 1 diabetes; islets are isolated from a donor for subsequent culture prior to transplantation. However, high seeding conditions cause unsolicited fusion of multiple spheroids, thereby limiting oxygen diffusion to induce hypoxic cell death. Here we introduce a culture dish incorporating a micropyramid-patterned surface to prevent the unsolicited fusion and oxygen-permeable bottom for optimal oxygen environment. A 400
Identifiants
pubmed: 36537072
doi: 10.1088/1758-5090/aca79a
doi:
Substances chimiques
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDDK NIH HHS
ID : R03 DK129958
Pays : United States
Informations de copyright
© 2022 IOP Publishing Ltd.