Single-cell sortChIC identifies hierarchical chromatin dynamics during hematopoiesis.
Journal
Nature genetics
ISSN: 1546-1718
Titre abrégé: Nat Genet
Pays: United States
ID NLM: 9216904
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
received:
22
05
2021
accepted:
01
11
2022
pubmed:
21
12
2022
medline:
16
2
2023
entrez:
20
12
2022
Statut:
ppublish
Résumé
Post-translational histone modifications modulate chromatin activity to affect gene expression. How chromatin states underlie lineage choice in single cells is relatively unexplored. We develop sort-assisted single-cell chromatin immunocleavage (sortChIC) and map active (H3K4me1 and H3K4me3) and repressive (H3K27me3 and H3K9me3) histone modifications in the mouse bone marrow. During differentiation, hematopoietic stem and progenitor cells (HSPCs) acquire active chromatin states mediated by cell-type-specifying transcription factors, which are unique for each lineage. By contrast, most alterations in repressive marks during differentiation occur independent of the final cell type. Chromatin trajectory analysis shows that lineage choice at the chromatin level occurs at the progenitor stage. Joint profiling of H3K4me1 and H3K9me3 demonstrates that cell types within the myeloid lineage have distinct active chromatin but share similar myeloid-specific heterochromatin states. This implies a hierarchical regulation of chromatin during hematopoiesis: heterochromatin dynamics distinguish differentiation trajectories and lineages, while euchromatin dynamics reflect cell types within lineages.
Identifiants
pubmed: 36539617
doi: 10.1038/s41588-022-01260-3
pii: 10.1038/s41588-022-01260-3
pmc: PMC9925381
doi:
Substances chimiques
Chromatin
0
Heterochromatin
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
333-345Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022. The Author(s).
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