Genomic and phylogenetic characterization of wheat yellows virus, a novel tenuivirus infecting wheat in South Africa.


Journal

Archives of virology
ISSN: 1432-8798
Titre abrégé: Arch Virol
Pays: Austria
ID NLM: 7506870

Informations de publication

Date de publication:
20 Dec 2022
Historique:
received: 01 07 2022
accepted: 01 09 2022
entrez: 20 12 2022
pubmed: 21 12 2022
medline: 23 12 2022
Statut: epublish

Résumé

During the 2019 winter wheat season, symptoms of severe chlorosis and stunting were observed on wheat in the irrigation production areas of South Africa. RNAtag-seq data were generated for seven samples from KwaZulu-Natal province and one from Limpopo. Analysis of assembled contigs indicated the presence of a putatively novel member of the genus Tenuivirus, tentatively named "wheat yellows virus" (WhYV). The genome is made up of four segments, which are 8952, 3451, 2338, and 2045 nucleotides in length and code for a total of seven ORFs. Phylogenies of each segment (nucleotide) and the polymerase gene (amino acid), as well as amino acid sequence comparisons of each gene product, showed that WhYV is most closely related to rice stripe virus.

Identifiants

pubmed: 36539651
doi: 10.1007/s00705-022-05649-7
pii: 10.1007/s00705-022-05649-7
doi:

Substances chimiques

Nucleotides 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

3

Informations de copyright

© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.

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Auteurs

David A Read (DA)

Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria, South Africa. david.read@fabi.up.ac.za.
Agricultural Research Council (ARC), Biotechnology Platform, 100 Old Soutpan Road, Onderstepoort, Pretoria, 0110, South Africa. david.read@fabi.up.ac.za.

Ronel Roberts (R)

ARC, Tropical and Subtropical Crops, Private Bag X11208, Mbombela, 1200, South Africa.

Goddy Prinsloo (G)

ARC, Small Grain, Private Bag X29, Bethlehem, 9700, South Africa.

Genevieve D Thompson (GD)

Gene Vantage, 34 Monte Carlo Crescent, Kyalami Business Park, Johannesburg, 1684, South Africa.

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