Preparation of collagen fibrils from mineralized tissues and evaluation by atomic force microscopy.
Atomic force microscopy
Collagen
Dentin
Hard tissues
Mechanical properties
Journal
Journal of the mechanical behavior of biomedical materials
ISSN: 1878-0180
Titre abrégé: J Mech Behav Biomed Mater
Pays: Netherlands
ID NLM: 101322406
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
received:
18
10
2022
revised:
01
12
2022
accepted:
11
12
2022
pmc-release:
01
02
2024
pubmed:
22
12
2022
medline:
19
1
2023
entrez:
21
12
2022
Statut:
ppublish
Résumé
Mineralized tissues like bone and dentin are materials that support the distribution of mechanical loads through the body of humans and other animals. While their organic content plays a critical role on the structural behavior of these materials, investigations that quantify the structural properties of collagen fibrils in mineralized tissues at the nanoscale are rather limited. We report a new experimental methodology to prepare samples of dentinal collagen fibrils for evaluation by atomic force microscopy and characterize their mechanical behavior. Specifically, a Dynamic Mechanical Analysis (DMA) of the collagen fibrils was performed to study their viscoelastic behavior. The capacity for viscous dampening in the fibrils was characterized in terms of measures of the energy dissipation, phase angle and loss modulus in both the peak and trough regions of the fibrils. According to the phase angle and the loss modulus, the peak regions of the fibrils exhibit significantly greater stiffness and capacity for dampening than the trough regions. This new approach will help in exploring the role of collagen fibrils in the mechanical behavior of dentin and other mineralized tissues as well as help to understand the potential effects from changes in fibril confirmation with tissue treatments, aging or that result from chronic disease.
Identifiants
pubmed: 36543081
pii: S1751-6161(22)00529-X
doi: 10.1016/j.jmbbm.2022.105624
pmc: PMC9845140
mid: NIHMS1861978
pii:
doi:
Substances chimiques
Collagen
9007-34-5
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105624Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE016904
Pays : United States
Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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