Fabrication of a novel electrochemical biosensor based on a molecular imprinted polymer-aptamer hybrid receptor for lysozyme determination.
Electrochemical biosensor
Lysozyme
MIP-aptamer hybrid receptor
Methylene blue
Nitrogen-doped carbon quantum dots
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
20
10
2022
accepted:
13
12
2022
revised:
19
11
2022
pubmed:
22
12
2022
medline:
1
2
2023
entrez:
21
12
2022
Statut:
ppublish
Résumé
In this work, a novel, sensitive, and rapid electrochemical biosensor was employed to detect lysozyme (Lys) using a double receptor of molecular imprinted polymer (MIP)-aptamer. First, a glassy carbon electrode (GCE) was modified with a nanocomposite consisting of multi-wall carbon nanotubes (MWCNTs), nitrogen-doped carbon quantum dots (N-CQDs), and chitosan. Subsequently, aptamer (Apt)-Lys complex was immobilized on MWCNTs-N-CQDs-chitosan/GCE via binding between carboxyl groups present in the nanocomposite and the terminal amine groups of the aptamer. Following that, methylene blue monomer was electrochemically polymerized around the Apt-Lys complex on the MWCNTs-N-CQDs-chitosan/GCE surface. Finally, after the template removal, the remaining cavities along with the aptamers created a new hybrid receptor of MIP-aptamer. The MWCNTs-N-CQDs-chitosan nanocomposite could provide large amounts of carboxyl groups for binding to amino-functionalized aptamers, considerable electrical conductivity, and a high surface-to-volume ratio. These beneficial features facilitated the Apt-Lys complex immobilization and gave improved electrochemical signal. The obtained MIP-aptamer hybrid receptor allowed lysozyme determination even at concentrations as low as 4.26 fM within the functional range of 1 fM to 100 nM.
Identifiants
pubmed: 36544030
doi: 10.1007/s00216-022-04487-5
pii: 10.1007/s00216-022-04487-5
doi:
Substances chimiques
Chitosan
9012-76-4
Aptamers, Nucleotide
0
Polymers
0
Muramidase
EC 3.2.1.17
Nanotubes, Carbon
0
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
899-911Subventions
Organisme : Babol Noshirvani University of Technology
ID : BNUT/965115004/1401
Organisme : Iran National Science Foundation
ID : 98020065
Informations de copyright
© 2022. Springer-Verlag GmbH Germany, part of Springer Nature.
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