Endothelial PlexinD1 signaling instructs spinal cord vascularization and motor neuron development.
CNS vascularization
PlexinD1
Sema3C
blood vessel
endothelial cell
motor neuron
neuro-vascular communication
neurodevelopment
spinal cord
Journal
Neuron
ISSN: 1097-4199
Titre abrégé: Neuron
Pays: United States
ID NLM: 8809320
Informations de publication
Date de publication:
21 12 2022
21 12 2022
Historique:
received:
25
05
2022
revised:
04
11
2022
accepted:
03
12
2022
entrez:
22
12
2022
pubmed:
23
12
2022
medline:
27
12
2022
Statut:
ppublish
Résumé
How the vascular and neural compartment cooperate to achieve such a complex and highly specialized structure as the central nervous system is still unclear. Here, we reveal a crosstalk between motor neurons (MNs) and endothelial cells (ECs), necessary for the coordinated development of MNs. By analyzing cell-to-cell interaction profiles of the mouse developing spinal cord, we uncovered semaphorin 3C (Sema3C) and PlexinD1 as a communication axis between MNs and ECs. Using cell-specific knockout mice and in vitro assays, we demonstrate that removal of Sema3C in MNs, or its receptor PlexinD1 in ECs, results in premature and aberrant vascularization of MN columns. Those vascular defects impair MN axon exit from the spinal cord. Impaired PlexinD1 signaling in ECs also causes MN maturation defects at later stages. This study highlights the importance of a timely and spatially controlled communication between MNs and ECs for proper spinal cord development.
Identifiants
pubmed: 36549270
pii: S0896-6273(22)01078-9
doi: 10.1016/j.neuron.2022.12.005
pmc: PMC9796814
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4074-4089.e6Subventions
Organisme : Wellcome Trust
ID : 205099/Z/16/Z
Pays : United Kingdom
Organisme : British Heart Foundation
ID : FS/19/29/34367
Pays : United Kingdom
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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