The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence.
Journal
Nature plants
ISSN: 2055-0278
Titre abrégé: Nat Plants
Pays: England
ID NLM: 101651677
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
30
11
2021
accepted:
28
10
2022
pubmed:
23
12
2022
medline:
27
1
2023
entrez:
22
12
2022
Statut:
ppublish
Résumé
Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.
Identifiants
pubmed: 36550363
doi: 10.1038/s41477-022-01302-y
pii: 10.1038/s41477-022-01302-y
pmc: PMC9873569
doi:
Substances chimiques
Abscisic Acid
72S9A8J5GW
Arabidopsis Proteins
0
Calcium Channels
0
Bacterial Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
128-141Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : No. 427105396
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : SFB 1101 project D08
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : LA 1338/9-1
Informations de copyright
© 2022. The Author(s).
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