Differential Mobilization of the Phospholipid and Triacylglycerol Pools of Arachidonic Acid in Murine Macrophages.
arachidonic acid
inflammation
membrane phospholipid
monocytes/macrophages
phospholipase A2
triacylglycerol
Journal
Biomolecules
ISSN: 2218-273X
Titre abrégé: Biomolecules
Pays: Switzerland
ID NLM: 101596414
Informations de publication
Date de publication:
11 12 2022
11 12 2022
Historique:
received:
23
11
2022
revised:
06
12
2022
accepted:
09
12
2022
entrez:
23
12
2022
pubmed:
24
12
2022
medline:
27
12
2022
Statut:
epublish
Résumé
Innate immune cells such as monocytes and macrophages contain high levels of arachidonic acid (AA), part of which can be mobilized during cellular activation for the formation of a vast array of bioactive oxygenated metabolites. Monocytes and macrophages present in inflammatory foci typically incorporate large amounts of AA, not only in membrane phospholipids, but also in neutral lipids such as triacylglycerol. Thus, it was of interest to investigate the metabolic fate of these two AA pools in macrophages. Utilizing a variety of radiolabeling techniques to distinguish the phospholipid and triacylglycerol pools, we show in this paper that during an acute stimulation of the macrophages with yeast-derived zymosan, the membrane phospholipid AA pool acts as the major, if not the only, source of releasable AA. On the contrary, the AA pool in triacylglycerol appears to be used at a later stage, when the zymosan-stimulated response has declined, as a source to replenish the phospholipid pools that were consumed during the activation process. Thus, phospholipids and triacylglycerol play different in roles AA metabolism and dynamics during macrophage activation.
Identifiants
pubmed: 36551279
pii: biom12121851
doi: 10.3390/biom12121851
pmc: PMC9775050
pii:
doi:
Substances chimiques
Arachidonic Acid
27YG812J1I
Phospholipids
0
Zymosan
9010-72-4
Triglycerides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministry of Science and Innovation
ID : PID2019-105989RB-I00
Organisme : Junta de Castilla y León
ID : CSI141P20
Organisme : Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
ID : CB07/08/0004
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