Combination of STING and TLR 7/8 Agonists as Vaccine Adjuvants for Cancer Immunotherapy.

DMXAA STING cancer immunotherapy cancer vaccine toll-like receptors

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
11 Dec 2022
Historique:
received: 14 11 2022
revised: 30 11 2022
accepted: 07 12 2022
entrez: 23 12 2022
pubmed: 24 12 2022
medline: 24 12 2022
Statut: epublish

Résumé

Immunostimulatory adjuvants that potently activate antigen-presenting cells and (in turn) prime cytotoxic T cells are a key component of anticancer vaccines. In this study, we investigated a multi-adjuvant approach combining a TLR 7/8 agonist (522) and a STING agonist (DMXAA) to promote enhanced antigen cross-presentation, stimulate specific antitumor T-cell responses, and provide improved anticancer efficacy. In vitro experiments using bone marrow-derived dendritic cells (BMDCs) confirmed enhanced activation with the 522-DMXAA combination based on both co-stimulatory molecule expression and pro-inflammatory cytokine secretion. The immunization of mice with vaccines comprising both 522 and DMXAA resulted in greater antitumor efficacy in B16F10 melanoma and MB49 bladder tumor models relative to mono-agonist vaccines. Flow cytometry-based analysis of immune cells from immunized mice revealed the significant activation of antigen-presenting cells, increased numbers of activated and Ag-specific CD8+ T cells in the spleen and lymph nodes, modest NK cell activation, and an overall reduction in CD206

Identifiants

pubmed: 36551577
pii: cancers14246091
doi: 10.3390/cancers14246091
pmc: PMC9777055
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : NCI NIH HHS
ID : R01 CA260825
Pays : United States
Organisme : NCI NIH HHS
ID : CA260825
Pays : United States

Références

Nat Rev Immunol. 2012 Jul 13;12(8):557-69
pubmed: 22790179
Nat Rev Immunol. 2022 Mar;22(3):158-172
pubmed: 34155388
Immunity. 2014 Nov 20;41(5):830-42
pubmed: 25517615
J Immunol. 2013 Nov 1;191(9):4475-87
pubmed: 24141854
Nature. 1997 Jul 24;388(6640):394-7
pubmed: 9237759
Nat Biomed Eng. 2018 Aug;2(8):578-588
pubmed: 31015631
Cancer Med. 2018 Sep;7(9):4509-4516
pubmed: 30039553
Oncoimmunology. 2020 Jul 21;9(1):1796002
pubmed: 32934889
Cell Rep. 2015 May 19;11(7):1018-30
pubmed: 25959818
ACS Med Chem Lett. 2017 Oct 16;8(11):1148-1152
pubmed: 29152046
Expert Opin Investig Drugs. 2010 Feb;19(2):295-304
pubmed: 20050824
J Clin Oncol. 2011 Aug 1;29(22):2965-71
pubmed: 21709202
Biomaterials. 2018 May;164:38-53
pubmed: 29482062
J Med Chem. 2014 Jan 23;57(2):339-47
pubmed: 24383475
Cells. 2022 Mar 29;11(7):
pubmed: 35406723
Int J Biol Sci. 2012;8(9):1237-47
pubmed: 23136552
J Immunol. 2017 Aug 1;199(3):941-954
pubmed: 28652396
Immunol Rev. 2019 Jul;290(1):24-38
pubmed: 31355488
Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19902-7
pubmed: 24248387
Vaccine. 2011 Apr 12;29(17):3341-55
pubmed: 20713100
J Immunol. 2020 Jul 1;205(1):223-236
pubmed: 32471881
Nanoscale. 2018 Nov 15;10(44):20851-20862
pubmed: 30403212
Expert Rev Vaccines. 2007 Apr;6(2):133-40
pubmed: 17408363
Immunol Lett. 2018 Apr;196:11-21
pubmed: 29407608
Front Immunol. 2014 Sep 25;5:461
pubmed: 25309543
Theranostics. 2019 Oct 15;9(25):7759-7771
pubmed: 31695799
J Immunother Cancer. 2016 Sep 20;4:56
pubmed: 27660710
Sci Rep. 2021 Feb 8;11(1):3346
pubmed: 33558639
BioDrugs. 1997 Aug;8(2):119-27
pubmed: 18020500
J Immunother Cancer. 2019 Apr 29;7(1):115
pubmed: 31036082
Trends Mol Med. 2008 Mar;14(3):109-19
pubmed: 18261959
Cell Mol Life Sci. 2010 May;67(10):1643-51
pubmed: 20229126
Chem Biol Drug Des. 2018 May;91(5):996-1006
pubmed: 29288534
Sci Rep. 2018 May 9;8(1):7355
pubmed: 29743548
Biomark Res. 2020 Sep 29;8:49
pubmed: 33005420
Eur J Immunol. 2015 Apr;45(4):1159-69
pubmed: 25529558
J Immunol. 2018 Apr 15;200(8):2748-2756
pubmed: 29540580
Cold Spring Harb Symp Quant Biol. 1989;54 Pt 1:1-13
pubmed: 2700931
Signal Transduct Target Ther. 2020 Jun 10;5(1):91
pubmed: 32532954
J Immunol. 2013 May 15;190(10):5216-25
pubmed: 23585680
J Immunol. 2012 May 15;188(10):4866-75
pubmed: 22504644
Nature. 2008 Jul 24;454(7203):436-44
pubmed: 18650914
Cancer Immunol Immunother. 2014 May;63(5):419-35
pubmed: 24514955
Int J Radiat Oncol Biol Phys. 2002 Dec 1;54(5):1503-11
pubmed: 12459378
Int J Hyperthermia. 2013 Aug;29(5):464-73
pubmed: 23862980
Lancet Oncol. 2003 Mar;4(3):141-8
pubmed: 12623359
J Immunother Cancer. 2021 Feb;9(2):
pubmed: 33526609
Nat Commun. 2017 Dec 22;8(1):2256
pubmed: 29273790
J Clin Invest. 2012 Nov;122(11):4094-104
pubmed: 23064360
Proc Natl Acad Sci U S A. 2016 Nov 15;113(46):E7240-E7249
pubmed: 27799536
Cell. 1994 Jan 28;76(2):275-85
pubmed: 7904901
Oncoimmunology. 2018 Oct 11;7(12):e1526250
pubmed: 30524908
Front Immunol. 2019 Jun 11;10:1289
pubmed: 31244840
Br J Cancer. 2013 Apr 2;108(6):1288-97
pubmed: 23481183
Mol Pharm. 2020 Jun 1;17(6):2109-2124
pubmed: 32383885
Sci Signal. 2012 Mar 06;5(214):ra20
pubmed: 22394562
Hematol Oncol Clin North Am. 2019 Apr;33(2):199-214
pubmed: 30832995
J Am Acad Dermatol. 2004 May;50(5):714-21
pubmed: 15097955
Semin Immunol. 2014 Feb;26(1):38-47
pubmed: 24602448
Sci Rep. 2021 Nov 11;11(1):22102
pubmed: 34764332
Cytokine. 2018 Jan;101:14-18
pubmed: 27531077
Cell. 2016 Oct 6;167(2):397-404.e9
pubmed: 27667683
Cell Res. 2006 Feb;16(2):141-7
pubmed: 16474426
Cancers (Basel). 2021 Aug 04;13(16):
pubmed: 34439079

Auteurs

Shubhmita Bhatnagar (S)

Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA.

Vishnu Revuri (V)

Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA.

Manan Shah (M)

Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA.
Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

Peter Larson (P)

Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

Zekun Shao (Z)

Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA.
Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

Daohai Yu (D)

Center for Biostatistics and Epidemiology, Lewis-Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.

Swayam Prabha (S)

Fels Cancer Institute for Personalized Medicine, Lewis-Katz School of Medicine, Temple University, Philadelphia, PA 19140, USA.
Fox Chase Comprehensive Cancer Institute, Temple University, Philadelphia, PA 19111, USA.

Thomas S Griffith (TS)

Department of Urology, Medical School, University of Minnesota, Minneapolis, MN 55455, USA.

David Ferguson (D)

Department of Medicinal Chemistry, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.

Jayanth Panyam (J)

Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA.
Department of Pharmaceutics, College of Pharmacy, University of Minnesota, Minneapolis, MN 55455, USA.
Fox Chase Comprehensive Cancer Institute, Temple University, Philadelphia, PA 19111, USA.

Classifications MeSH