Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cells.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
06 03 2023
06 03 2023
Historique:
received:
04
03
2022
revised:
20
10
2022
accepted:
01
12
2022
entrez:
23
12
2022
pubmed:
24
12
2022
medline:
28
12
2022
Statut:
ppublish
Résumé
Septins are cytoskeletal proteins conserved from algae and protists to mammals. A unique feature of septins is their presence as heteromeric complexes that polymerize into filaments in solution and on lipid membranes. Although animal septins associate extensively with actin-based structures in cells, whether septins organize as filaments in cells and if septin organization impacts septin function is not known. Customizing a tripartite split-GFP complementation assay, we show that all septins decorating actin stress fibers are octamer-containing filaments. Depleting octamers or preventing septins from polymerizing leads to a loss of stress fibers and reduced cell stiffness. Super-resolution microscopy revealed septin fibers with widths compatible with their organization as paired septin filaments. Nanometer-resolved distance measurements and single-protein tracking further showed that septin filaments are membrane bound and largely immobilized. Finally, reconstitution assays showed that septin filaments mediate actin-membrane anchoring. We propose that septin organization as octamer-based filaments is essential for septin function in anchoring and stabilizing actin filaments at the plasma membrane.
Identifiants
pubmed: 36562751
pii: 213778
doi: 10.1083/jcb.202203016
pmc: PMC9802686
pii:
doi:
Substances chimiques
Actins
0
Septins
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM122375
Pays : United States
Organisme : NIH HHS
ID : S10 OD018136
Pays : United States
Organisme : NCRR NIH HHS
ID : S10 RR026866
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2022 Silva Martins et al.
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