Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects.


Journal

Molecular nutrition & food research
ISSN: 1613-4133
Titre abrégé: Mol Nutr Food Res
Pays: Germany
ID NLM: 101231818

Informations de publication

Date de publication:
02 2023
Historique:
pubmed: 25 12 2022
medline: 25 2 2023
entrez: 24 12 2022
Statut: ppublish

Résumé

Peripheral blood mononuclear cells (PBMC) provide a useful and minimally invasive source of biomarkers. Here to identify PBMC transcriptomic biomarkers predictive of metabolic impairment related to increased adiposity is aimed. The study analyzed the global PBMC transcriptome in metabolically healthy (normoglycemic) volunteers with overweight-obesity (OW-OB, n = 12), and in subjects with metabolically obese normal-weight (MONW, n = 5) phenotype, in comparison to normal-weight (NW, n = 12) controls. The study identifies 1072 differentially expressed genes (DEGs) in OW-OB versus NW and 992 in MONW versus NW. Hierarchical clustering of the top 100 DEGs clearly distinguishes OW-OB and MONW from NW. Remarkably, the OW-OB and MONW phenotypes share 257 DEGs regulated in the same direction. The top up-regulated gene CXCL8, coding for interleukin 8, with a role in obesity-related pathologies, is of special interest as a potential marker for predicting increased metabolic risk. CXCL8 expression is increased mainly in the MONW group and correlated directly with C-reactive protein levels. PBMC gene expression analysis of CXCL8 or a pool of DEGs may be used to identify early metabolic risk in an apparently healthy population regardless of their BMI, i.e., subjects with OW-OB or MONW phenotype and to apply adequate and personalized nutritional preventive strategies.

Identifiants

pubmed: 36564895
doi: 10.1002/mnfr.202200503
doi:

Substances chimiques

Biomarkers 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2200503

Informations de copyright

© 2023 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.

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Auteurs

Andrea Costa (A)

Nutrigenomics, Biomarkers and Risk Evaluation (NuBE) group, University of the Balearic Islands (UIB), Palma, Mallorca, 07122, Spain.
Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.

Inge van der Stelt (I)

Human and Animal Physiology, Wageningen University, Wageningen, 6708, The Netherlands.

Bàrbara Reynés (B)

Nutrigenomics, Biomarkers and Risk Evaluation (NuBE) group, University of the Balearic Islands (UIB), Palma, Mallorca, 07122, Spain.
Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.

Jadwiga Konieczna (J)

Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.
Research Group on Nutritional Epidemiology & Cardiovascular Physiopathology (NUTRECOR), University Hospital Son Espases (HUSE), Palma, Mallorca, 07120, Spain.

Miquel Fiol (M)

Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.
Research Group on Nutritional Epidemiology & Cardiovascular Physiopathology (NUTRECOR), University Hospital Son Espases (HUSE), Palma, Mallorca, 07120, Spain.

Jaap Keijer (J)

Human and Animal Physiology, Wageningen University, Wageningen, 6708, The Netherlands.

Andreu Palou (A)

Nutrigenomics, Biomarkers and Risk Evaluation (NuBE) group, University of the Balearic Islands (UIB), Palma, Mallorca, 07122, Spain.
Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.

Dora Romaguera (D)

Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.
Research Group on Nutritional Epidemiology & Cardiovascular Physiopathology (NUTRECOR), University Hospital Son Espases (HUSE), Palma, Mallorca, 07120, Spain.

Evert M van Schothorst (EM)

Human and Animal Physiology, Wageningen University, Wageningen, 6708, The Netherlands.

Paula Oliver (P)

Nutrigenomics, Biomarkers and Risk Evaluation (NuBE) group, University of the Balearic Islands (UIB), Palma, Mallorca, 07122, Spain.
Health Research Institute of the Balearic Islands (IdISBa), Palma, Mallorca, 07010, Spain.
CIBER of Physiopathology of Obesity and Nutrition (CIBEROBN), Instituto de Salud Carlos III, Madrid, 28029, Spain.

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