Transcriptome-wide identification of development related genes and pathways in Tribolium castaneum.
Cell cycle
DNA replication
Ecdysteroids
Juvenile hormone
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
received:
30
05
2022
revised:
07
11
2022
accepted:
20
12
2022
pubmed:
26
12
2022
medline:
18
1
2023
entrez:
25
12
2022
Statut:
ppublish
Résumé
The growth and development in Tribolium castaneum were poorly understood at the transcriptome level. Currently, we identified 15,756, 9941 and 10,080 differentially expressed transcripts between late eggs VS early larvae, late larvae VS early pupae, and late pupae VS early adults of T. castaneum by RNA-seq, which was confirmed by qRT-PCR analysis on nine genes expression. Functional enrichment analysis indicated that DNA replication, cell cycle and insect hormone biosynthesis significantly enriched differentially expressed genes. The transcription of DNA replication and cell cycle genes decreased after hatching but increased after pupation. The juvenile hormone (JH) and ecdysteroid biosynthesis genes decreased after hatching, and the JH degradation genes were stimulated after pupation and eclosion while the ecdysteroid degradation gene CYP18A1 decreased after pupation. Silencing CYP18A1 elevated the titer of ecdysteroids and caused developmental arrest at the late larval stage. This study promotes the understanding of insect growth and development.
Identifiants
pubmed: 36566947
pii: S0888-7543(22)00298-1
doi: 10.1016/j.ygeno.2022.110551
pii:
doi:
Substances chimiques
Ecdysteroids
0
Insect Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110551Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.