Five-Minute Magnetic Nanoparticle Spectroscopy-Based Bioassay for Ultrafast Detection of SARS-CoV-2 Spike Protein.
Journal
ACS applied nano materials
ISSN: 2574-0970
Titre abrégé: ACS Appl Nano Mater
Pays: United States
ID NLM: 101726750
Informations de publication
Date de publication:
23 Dec 2022
23 Dec 2022
Historique:
received:
06
12
2022
accepted:
09
12
2022
pmc-release:
23
12
2023
entrez:
26
12
2022
pubmed:
27
12
2022
medline:
27
12
2022
Statut:
ppublish
Résumé
In this work, we report a 5-min magnetic particle spectroscopy (MPS)-based bioassay strategy. In our approach, surface-functionalized magnetic nanoparticles are incubated with target analytes at 37 °C with agitation for 3 min, and the MPS reading is then taken at the fifth minute. We prove the feasibility of 5 min ultrafast detection of SARS-CoV-2 spike protein with a detection limit below 5 nM (0.2 pmol). Our proposed 5-min bioassay strategy may be applied to reduce the assay time for other liquid-phase, volumetric biosensors such as NMR, quantum dots, fluorescent biosensors, etc.
Identifiants
pubmed: 36570474
doi: 10.1021/acsanm.2c05237
pmc: PMC9762417
mid: NIHMS1922605
doi:
Types de publication
Journal Article
Langues
eng
Pagination
17503-17507Subventions
Organisme : NIDCR NIH HHS
ID : R42 DE030832
Pays : United States
Informations de copyright
© 2022 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
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