Multimodal monitoring of human cortical organoids implanted in mice reveal functional connection with visual cortex.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 12 2022
26 12 2022
Historique:
received:
04
09
2021
accepted:
09
12
2022
entrez:
26
12
2022
pubmed:
27
12
2022
medline:
29
12
2022
Statut:
epublish
Résumé
Human cortical organoids, three-dimensional neuronal cultures, are emerging as powerful tools to study brain development and dysfunction. However, whether organoids can functionally connect to a sensory network in vivo has yet to be demonstrated. Here, we combine transparent microelectrode arrays and two-photon imaging for longitudinal, multimodal monitoring of human cortical organoids transplanted into the retrosplenial cortex of adult mice. Two-photon imaging shows vascularization of the transplanted organoid. Visual stimuli evoke electrophysiological responses in the organoid, matching the responses from the surrounding cortex. Increases in multi-unit activity (MUA) and gamma power and phase locking of stimulus-evoked MUA with slow oscillations indicate functional integration between the organoid and the host brain. Immunostaining confirms the presence of human-mouse synapses. Implantation of transparent microelectrodes with organoids serves as a versatile in vivo platform for comprehensive evaluation of the development, maturation, and functional integration of human neuronal networks within the mouse brain.
Identifiants
pubmed: 36572698
doi: 10.1038/s41467-022-35536-3
pii: 10.1038/s41467-022-35536-3
pmc: PMC9792589
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7945Subventions
Organisme : NINDS NIH HHS
ID : U19 NS123717
Pays : United States
Organisme : NIBIB NIH HHS
ID : R21 EB026180
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH109885
Pays : United States
Organisme : NEI NIH HHS
ID : R21 EY029466
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007198
Pays : United States
Organisme : NIBIB NIH HHS
ID : DP2 EB030992
Pays : United States
Informations de copyright
© 2022. The Author(s).
Références
Nature. 1946 Oct 19;158(4016):540
pubmed: 21001939
J Neurophysiol. 2008 Mar;99(3):1461-76
pubmed: 18160425
Science. 2000 Nov 17;290(5495):1364-8
pubmed: 11082065
J Neurophysiol. 2018 Nov 1;120(5):2232-2245
pubmed: 30067128
PLoS Biol. 2016 Feb 18;14(2):e1002383
pubmed: 26890123
Nat Commun. 2018 May 23;9(1):2035
pubmed: 29789548
IEEE Trans Biomed Eng. 2020 Nov;67(11):3203-3210
pubmed: 32191878
Science. 2000 Feb 25;287(5457):1433-8
pubmed: 10688783
Lab Anim. 1999 Jul;33(3):243-51
pubmed: 10780843
Neuron. 2011 Dec 8;72(5):847-58
pubmed: 22153379
Biophys J. 2008 Feb 1;94(3):784-802
pubmed: 17921225
Nat Biotechnol. 2018 Jun;36(5):432-441
pubmed: 29658944
Nat Phys. 2018 May;14(5):515-522
pubmed: 29760764
Cell Stem Cell. 2019 Oct 3;25(4):558-569.e7
pubmed: 31474560
J Neurosci. 2016 Nov 30;36(48):12259-12275
pubmed: 27903733
Science. 1984 Sep 7;225(4666):1046-8
pubmed: 6474166
Nat Biotechnol. 2020 Dec;38(12):1421-1430
pubmed: 33273741
Nat Rev Neurosci. 2017 Oct;18(10):573-584
pubmed: 28878372
PLoS Biol. 2015 Aug 14;13(8):e1002231
pubmed: 26274866
Cell. 2020 Dec 23;183(7):1913-1929.e26
pubmed: 33333020
J Neurosci Methods. 2010 Sep 30;192(1):146-51
pubmed: 20637804
Nat Biomed Eng. 2020 Oct;4(10):973-983
pubmed: 32719512
Cell Stem Cell. 2014 Nov 6;15(5):559-73
pubmed: 25517465
Front Syst Neurosci. 2008 Jun 17;2:2
pubmed: 18958246
J Neurophysiol. 2005 Jul;94(1):479-90
pubmed: 15703230
Prog Brain Res. 2005;147:263-82
pubmed: 15581712
J Neurosci. 2013 Jan 23;33(4):1535-9
pubmed: 23345227
Front Syst Neurosci. 2016 Jan 08;9:175
pubmed: 26778976
Nat Neurosci. 2013 Oct;16(10):1426-35
pubmed: 23974708
Stem Cell Reports. 2017 Dec 12;9(6):1745-1753
pubmed: 29198827
Trends Mol Med. 2018 Dec;24(12):982-990
pubmed: 30377071
Nat Rev Neurosci. 2013 Nov;14(11):770-85
pubmed: 24135696
Neuroscience. 2013 Dec 19;254:141-51
pubmed: 24035827
Cell Rep. 2018 Mar 13;22(11):2873-2885
pubmed: 29539417
Nat Rev Neurosci. 2012 May 18;13(6):407-20
pubmed: 22595786
Neuron. 2011 May 26;70(4):687-702
pubmed: 21609825
Front Neurosci. 2020 Oct 27;14:559077
pubmed: 33192246
ACS Nano. 2011 Dec 27;5(12):9927-33
pubmed: 22034835
J Cereb Blood Flow Metab. 2020 Mar;40(3):501-512
pubmed: 30829101
Nat Neurosci. 2015 Feb;18(2):310-5
pubmed: 25531570
Nature. 2017 May 4;545(7652):48-53
pubmed: 28445462
Stem Cell Reports. 2020 Aug 11;15(2):467-481
pubmed: 32679062
Nat Neurosci. 2015 Jun;18(6):892-902
pubmed: 25915477
Nat Neurosci. 2021 Mar;24(3):331-342
pubmed: 33619405
Front Psychol. 2021 Jun 10;12:673529
pubmed: 34177731
Neuron. 2014 Aug 20;83(4):789-96
pubmed: 25123310
Neuron. 2017 Jan 18;93(2):315-322
pubmed: 28103479
Nat Biotechnol. 2017 Jul;35(7):659-666
pubmed: 28562594
Int J Psychophysiol. 2006 Aug;61(2):262-78
pubmed: 16377013
Neuroimage. 2007 Feb 15;34(4):1443-9
pubmed: 17188898
J Neurosci. 2015 Jul 1;35(26):9603-14
pubmed: 26134643
J Neurosci. 2008 Nov 5;28(45):11526-36
pubmed: 18987189
J Neurophysiol. 2003 Aug;90(2):1115-23
pubmed: 12702711
Behav Brain Res. 2002 Apr 1;131(1-2):105-14
pubmed: 11844577
Eur J Neurosci. 1998 May;10(5):1856-77
pubmed: 9751156
Nat Methods. 2016 Oct;13(10):875-82
pubmed: 27571550
J Neurosci Methods. 2004 Mar 15;134(1):9-21
pubmed: 15102499