Genome-wide distribution of histone trimethylation reveals a global impact of bisphenol A on telomeric binding proteins and histone acetyltransferase factors: a pilot study with human and in vitro data.
Bisphenol A
ChIP-seq
DNA methylation
H3K4me3
Human blood
Telomere
Journal
Clinical epigenetics
ISSN: 1868-7083
Titre abrégé: Clin Epigenetics
Pays: Germany
ID NLM: 101516977
Informations de publication
Date de publication:
26 12 2022
26 12 2022
Historique:
received:
27
09
2022
accepted:
14
12
2022
entrez:
26
12
2022
pubmed:
27
12
2022
medline:
29
12
2022
Statut:
epublish
Résumé
To assess the genetic and epigenetic effects promoted by Bisphenol A (BPA) exposure in adolescent males from the Spanish INMA-Granada birth cohort, and in human cells. DNA methylation was analysed using MEDIP. Repeat number variation in genomic DNA was evaluated, along with the analysis of H3K4me3 by using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq). Analyses were performed with material extracted from whole blood of the adolescents, complemented by in vitro assessments of human (HeLa) cells exposed to 10 nM BPA, specifically, immunofluorescence evaluation of protein levels, gene expression analysis and ChIP‒qPCR analysis. Adolescents in the high urinary BPA levels group presented a higher level of Satellite A (SATA) repetitive region copy numbers compared to those in the low BPA group and a tendency towards increase in telomere length. We also observed decreased DNA methylation at the promoters of the imprinted genes H19, KCNQ1, and IGF2; at LINE1 retroelements; and at the ARID2, EGFR and ESRRA and TERT genes. Genome-wide sequencing revealed increased H3K4me3 occupancy at the promoters of genes encoding histone acetyltransferases, telomeric DNA binding factors and DNA repair genes. Results were supported in HeLa cells exposed to 10 nM BPA in vitro. In accordance with the data obtained in blood samples, we observed higher H3K4me3 occupancy and lower DNA methylation at some specific targets in HeLa cells. In exposed cells, changes in the expression of genes encoding DNA repair factors (ATM, ARID2, TRP53) were observed, and increased expression of several genes encoding telomeric DNA binding factors (SMG7, TERT, TEN1, UPF1, ZBTB48) were also found. Furthermore, an increase in ESR1/ERa was observed in the nuclei of HeLa cells along with increased binding of ESR1 to KAT5, KMT2E and TERF2IP promoters and decreased ESR1 binding at the RARA promoter. The DNA damage marker p53/TP53 was also increased. In this pilot study, genome-wide analysis of histone trimethylation in adolescent males exposed to BPA revealed a global impact on the expression of genes encoding telomeric binding proteins and histone acetyltransferase factors with similar results in HeLa cells. Nevertheless, larger studies should confirm our findings.
Identifiants
pubmed: 36572933
doi: 10.1186/s13148-022-01408-2
pii: 10.1186/s13148-022-01408-2
pmc: PMC9793539
doi:
Substances chimiques
Histones
0
bisphenol A
MLT3645I99
Telomere-Binding Proteins
0
DNA
9007-49-2
UPF1 protein, human
EC 3.6.4.13
Trans-Activators
0
RNA Helicases
EC 3.6.4.13
SMG7 protein, human
0
Carrier Proteins
0
ZBTB48 protein, human
0
DNA-Binding Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
186Informations de copyright
© 2022. The Author(s).
Références
Environ Health Perspect. 2009 May;117(5):784-9
pubmed: 19479022
Endocrinology. 2015 Jun;156(6):2049-58
pubmed: 25807043
Environ Toxicol Pharmacol. 2012 Jan;33(1):9-15
pubmed: 22047638
PLoS Genet. 2013 Apr;9(4):e1003401
pubmed: 23593014
Sci Rep. 2019 Dec 2;9(1):18029
pubmed: 31792261
Clin Chem. 2017 Apr;63(4):816-822
pubmed: 28188229
Nat Struct Mol Biol. 2020 Feb;27(2):168-178
pubmed: 32042151
Science. 2011 Feb 4;331(6017):593-6
pubmed: 21233348
Toxics. 2019 Aug 29;7(3):
pubmed: 31470548
Environ Int. 2021 Jun;151:106440
pubmed: 33640694
Bioinformatics. 2011 Jun 15;27(12):1696-7
pubmed: 21486936
J Clin Invest. 2022 Aug 15;132(16):
pubmed: 35968784
Bioinformatics. 2011 Apr 1;27(7):1017-8
pubmed: 21330290
Genome Biol. 2009;10(3):R25
pubmed: 19261174
Andrology. 2014 Jan;2(1):138-44
pubmed: 24293158
Blood. 2009 Sep 10;114(11):2236-43
pubmed: 19561322
Environ Health Perspect. 2007 Dec;115 Suppl 1:8-14
pubmed: 18174944
Environ Res. 2019 Jun;173:443-451
pubmed: 30974370
Mol Cell. 2007 May 11;26(3):323-34
pubmed: 17499040
Curr Biol. 1998 May 21;8(11):657-60
pubmed: 9635193
Cell Rep. 2018 May 22;23(8):2392-2404
pubmed: 29791850
Environ Res. 2019 Mar;170:406-415
pubmed: 30623888
BMC Med. 2021 Jul 22;19(1):166
pubmed: 34289836
Age (Dordr). 2016 Dec;38(5-6):405-418
pubmed: 27631330
Science. 2010 Mar 26;327(5973):1657-61
pubmed: 20339076
Neurotoxicology. 2015 Jul;49:174-84
pubmed: 26121921
Nucleic Acids Res. 2016 Mar 18;44(5):e45
pubmed: 26578583
Clin Endocrinol (Oxf). 2014 Jun;80(6):773-81
pubmed: 24593700
Biochim Biophys Acta. 2007 Jan;1769(1):29-40
pubmed: 17239456
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W280-6
pubmed: 15215395
Environ Res. 2022 Nov;214(Pt 2):113897
pubmed: 35839910
Mol Cell Biol. 2005 Jun;25(11):4650-61
pubmed: 15899867
Horm Cancer. 2010 Jun;1(3):146-55
pubmed: 21761357
Sci Rep. 2017 Jan 09;7:40337
pubmed: 28067316
Mol Biol Rep. 2012 Sep;39(9):8621-8
pubmed: 22699882
Endocrinology. 1993 Jun;132(6):2279-86
pubmed: 8504731
Ann Genet. 1994;37(4):163-71
pubmed: 7710250
Mol Cells. 2008 Nov 30;26(5):454-8
pubmed: 18719354
Proc Natl Acad Sci U S A. 2015 Jun 2;112(22):6807-13
pubmed: 25385582
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
J Biol Chem. 2002 Jan 18;277(3):1669-79
pubmed: 11700319
Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):13056-61
pubmed: 17670942
Genes (Basel). 2022 Feb 20;13(2):
pubmed: 35205427
Sci Total Environ. 2021 Jun 25;775:145755
pubmed: 34132197
BMC Med. 2020 Aug 19;18(1):243
pubmed: 32811491
Sci Total Environ. 2022 Jan 10;803:150014
pubmed: 34788942
Genetics. 1993 Aug;134(4):1149-74
pubmed: 8375654
EMBO J. 2009 Nov 4;28(21):3390-9
pubmed: 19763083
Environ Int. 2020 Jun;139:105692
pubmed: 32251899
Neurotoxicology. 2016 Mar;53:12-19
pubmed: 26654821
Food Chem Toxicol. 2012 Mar;50(3-4):1124-33
pubmed: 22142692
J Clin Invest. 2003 Jun;111(11):1651-64
pubmed: 12782668
Annu Rev Public Health. 2018 Apr 1;39:309-333
pubmed: 29328878
Oncogene. 2014 May 22;33(21):2681-9
pubmed: 23752187
Nat Biotechnol. 2011 Jan;29(1):24-6
pubmed: 21221095
Toxicol Lett. 2006 Dec 1;167(2):95-105
pubmed: 17049190
Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7
pubmed: 27141961
Am J Pathol. 2014 May;184(5):1280-6
pubmed: 24607009
Sci Rep. 2019 Aug 2;9(1):11259
pubmed: 31375789
EMBO J. 2021 Jan 15;40(2):e103654
pubmed: 33210317
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Biochem Biophys Res Commun. 2001 Aug 24;286(3):529-33
pubmed: 11511091
Endocr J. 1999 Dec;46(6):773-8
pubmed: 10724352
Chromosome Res. 2015 Sep;23(3):479-93
pubmed: 26293605
Environ Health Perspect. 2015 Dec;123(12):1271-9
pubmed: 25933419
Mol Cancer Res. 2018 Aug;16(8):1255-1262
pubmed: 29748382
Environ Res. 2003 Sep;93(1):31-5
pubmed: 12865045
Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14899-904
pubmed: 10611310
Nature. 2009 Aug 27;460(7259):1127-31
pubmed: 19657334
Nat Rev Genet. 2012 May 29;13(7):484-92
pubmed: 22641018
Genome Biol. 2007;8(2):R24
pubmed: 17324271
Environ Health Perspect. 2011 Jul;119(7):914-20
pubmed: 21450549
Hum Exp Toxicol. 2018 May;37(5):475-485
pubmed: 28597690
Life Sci Alliance. 2021 Apr 9;4(6):
pubmed: 33837044
Nucleic Acids Res. 2009 Oct;37(19):6340-54
pubmed: 19720732
J Hepatol. 2017 May;66(5):942-951
pubmed: 28238438
Carcinogenesis. 2008 Oct;29(10):1862-8
pubmed: 18544561
Environ Int. 2019 Jun;127:592-600
pubmed: 30986741
Chemosphere. 2019 May;222:235-242
pubmed: 30708157
Curr Pharm Des. 2004;10(23):2839-50
pubmed: 15379672
Environ Res. 2020 Mar;182:108958
pubmed: 31835118