Expression patterns of NKCC1 in neurons and non-neuronal cells during cortico-hippocampal development.
NKCC1
Slc12a2
bumetanide
chloride regulation
neurodevelopmental disorders
Journal
Cerebral cortex (New York, N.Y. : 1991)
ISSN: 1460-2199
Titre abrégé: Cereb Cortex
Pays: United States
ID NLM: 9110718
Informations de publication
Date de publication:
09 05 2023
09 05 2023
Historique:
received:
25
08
2022
revised:
05
11
2022
accepted:
06
11
2022
medline:
16
5
2023
pubmed:
28
12
2022
entrez:
27
12
2022
Statut:
ppublish
Résumé
The Na-K-2Cl cotransporter NKCC1 is widely expressed in cells within and outside the brain. However, our understanding of its roles in brain functions throughout development, as well as in neuropsychiatric and neurological disorders, has been severely hindered by the lack of reliable data on its developmental and (sub)cellular expression patterns. We provide here the first properly controlled analysis of NKCC1 protein expression in various cell types of the mouse brain using custom-made antibodies and an NKCC1 knock-out validated immunohistochemical procedure, with parallel data based on advanced mRNA approaches. NKCC1 protein and mRNA are expressed at remarkably high levels in oligodendrocytes. In immature neurons, NKCC1 protein was located in the somata, whereas in adult neurons, only NKCC1 mRNA could be clearly detected. NKCC1 immunoreactivity is also seen in microglia, astrocytes, developing pericytes, and in progenitor cells of the dentate gyrus. Finally, a differential expression of NKCC1 splice variants was observed, with NKCC1a predominating in non-neuronal cells and NKCC1b in neurons. Taken together, our data provide a cellular basis for understanding NKCC1 functions in the brain and enable the identification of major limitations and promises in the development of neuron-targeting NKCC1-blockers.
Identifiants
pubmed: 36573432
pii: 6960611
doi: 10.1093/cercor/bhac470
pmc: PMC10183754
doi:
Substances chimiques
Solute Carrier Family 12, Member 2
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5906-5923Informations de copyright
© The Author(s) 2022. Published by Oxford University Press.
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