Drug sensitivity profiling of 3D tumor tissue cultures in the pediatric precision oncology program INFORM.
Journal
NPJ precision oncology
ISSN: 2397-768X
Titre abrégé: NPJ Precis Oncol
Pays: England
ID NLM: 101708166
Informations de publication
Date de publication:
27 Dec 2022
27 Dec 2022
Historique:
received:
05
05
2022
accepted:
29
11
2022
entrez:
27
12
2022
pubmed:
28
12
2022
medline:
28
12
2022
Statut:
epublish
Résumé
The international precision oncology program INFORM enrolls relapsed/refractory pediatric cancer patients for comprehensive molecular analysis. We report a two-year pilot study implementing ex vivo drug sensitivity profiling (DSP) using a library of 75-78 clinically relevant drugs. We included 132 viable tumor samples from 35 pediatric oncology centers in seven countries. DSP was conducted on multicellular fresh tumor tissue spheroid cultures in 384-well plates with an overall mean processing time of three weeks. In 89 cases (67%), sufficient viable tissue was received; 69 (78%) passed internal quality controls. The DSP results matched the identified molecular targets, including BRAF, ALK, MET, and TP53 status. Drug vulnerabilities were identified in 80% of cases lacking actionable (very) high-evidence molecular events, adding value to the molecular data. Striking parallels between clinical courses and the DSP results were observed in selected patients. Overall, DSP in clinical real-time is feasible in international multicenter precision oncology programs.
Identifiants
pubmed: 36575299
doi: 10.1038/s41698-022-00335-y
pii: 10.1038/s41698-022-00335-y
pmc: PMC9794727
doi:
Types de publication
Journal Article
Langues
eng
Pagination
94Informations de copyright
© 2022. The Author(s).
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