Antigen Discovery for Next-Generation Pertussis Vaccines Using Immunoproteomics and Transposon-Directed Insertion Sequencing.

Animals Humans Whooping Cough / prevention & control Bordetella pertussis / genetics Antibodies, Bacterial Pertussis Vaccine Papio
Bordetella pertussis TraDIS antigen baboon immunization immunoproteomics vaccine whooping cough

Journal

The Journal of infectious diseases
ISSN: 1537-6613
Titre abrégé: J Infect Dis
Pays: United States
ID NLM: 0413675

Informations de publication

Date de publication:
14 02 2023
Historique:
received: 26 08 2022
accepted: 27 12 2022
pubmed: 29 12 2022
medline: 17 2 2023
entrez: 28 12 2022
Statut: ppublish

Résumé

Despite high vaccination rates, the United States has experienced a resurgence in reported cases of pertussis after switching to the acellular pertussis vaccine, indicating a need for improved vaccines that enhance infection control. Bordetella pertussis antigens recognized by convalescent-baboon serum and nasopharyngeal wash were identified by immunoproteomics and their subcellular localization predicted. Genes essential or important for persistence in the baboon airway were identified by transposon-directed insertion-site sequencing (TraDIS) analysis. In total, 314 B. pertussis antigens were identified by convalescent baboon serum and 748 by nasopharyngeal wash. Thirteen antigens were identified as immunogenic in baboons, essential for persistence in the airway by TraDIS, and membrane-localized: BP0840 (OmpP), Pal, OmpA2, BP1485, BamA, Pcp, MlaA, YfgL, BP2197, BP1569, MlaD, ComL, and BP0183. The B. pertussis antigens identified as immunogenic, essential for persistence in the airway, and membrane-localized warrant further investigation for inclusion in vaccines designed to reduce or prevent carriage of bacteria in the airway of vaccinated individuals.

Sections du résumé

BACKGROUND
Despite high vaccination rates, the United States has experienced a resurgence in reported cases of pertussis after switching to the acellular pertussis vaccine, indicating a need for improved vaccines that enhance infection control.
METHODS
Bordetella pertussis antigens recognized by convalescent-baboon serum and nasopharyngeal wash were identified by immunoproteomics and their subcellular localization predicted. Genes essential or important for persistence in the baboon airway were identified by transposon-directed insertion-site sequencing (TraDIS) analysis.
RESULTS
In total, 314 B. pertussis antigens were identified by convalescent baboon serum and 748 by nasopharyngeal wash. Thirteen antigens were identified as immunogenic in baboons, essential for persistence in the airway by TraDIS, and membrane-localized: BP0840 (OmpP), Pal, OmpA2, BP1485, BamA, Pcp, MlaA, YfgL, BP2197, BP1569, MlaD, ComL, and BP0183.
CONCLUSIONS
The B. pertussis antigens identified as immunogenic, essential for persistence in the airway, and membrane-localized warrant further investigation for inclusion in vaccines designed to reduce or prevent carriage of bacteria in the airway of vaccinated individuals.

Identifiants

DOI: 10.1093/infdis/jiac502 PMID: 36575950 PMC: PMC10169431
pubmed: 36575950
pii: 6963326
doi: 10.1093/infdis/jiac502
pmc: PMC10169431
doi:

Substances chimiques

Antibodies, Bacterial 0
Pertussis Vaccine 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

583-591

Subventions

Organisme : Wellcome Trust
ID : 098051
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : U01 AI141995
Pays : United States
Organisme : FDA HHS
Pays : United States
Organisme : NIH HHS
ID : 032521
Pays : United States
Organisme : Wellcome Trust
ID : 098051
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : 75N93019C00066
Pays : United States

Informations de copyright

Published by Oxford University Press on behalf of Infectious Diseases Society of America 2022.

Déclaration de conflit d'intérêts

Potential conflicts of interest. All authors: No reported conflicts of interest. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.

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Auteurs

Kelsey A Gregg (KA)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

Yihui Wang (Y)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

Jason Warfel (J)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

Elizabeth Schoenfeld (E)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

Ewa Jankowska (E)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

John F Cipollo (JF)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.
ORCID: 0000-0002-9663-8177

Matthew Mayho (M)

Wellcome Sanger Institute, Hinxton, United Kingdom.

Christine Boinett (C)

Wellcome Sanger Institute, Hinxton, United Kingdom.

Deepika Prasad (D)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

Timothy J Brickman (TJ)

Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.

Sandra K Armstrong (SK)

Department of Microbiology and Immunology, University of Minnesota, Minneapolis, Minnesota, USA.

Julian Parkhill (J)

Wellcome Sanger Institute, Hinxton, United Kingdom.

Ricardo Da Silva Antunes (R)

Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, California, USA.

Alessandro Sette (A)

Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, California, USA.
Department of Medicine, University of California, San Diego, La Jolla, California, USA.

James F Papin (JF)

Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.

Roman Wolf (R)

Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, USA.

Tod J Merkel (TJ)

Division of Bacterial, Parasitic, and Allergenic Products, Center for Biologics Evaluation and Research, US Food and Drug Administration, Silver Spring, Maryland, USA.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Antigen Discovery for Next-Generation...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Antigen Discovery for Next-Generation...
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Coqueluche › Antigen Discovery for Next-Generation...
questionsmedicales.fr › Bactéries › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella › Bordetella pertussis › Antigen Discovery for Next-Generation...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antibactériens › Antigen Discovery for Next-Generation...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antibactériens › Vaccin anticoquelucheux › Antigen Discovery for Next-Generation...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Cercopithecidae › Cercopithecinae › Papio › Antigen Discovery for Next-Generation...