Genome-wide Interaction Study with Smoking for Colorectal Cancer Risk Identifies Novel Genetic Loci Related to Tumor Suppression, Inflammation, and Immune Response.


Journal

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
ISSN: 1538-7755
Titre abrégé: Cancer Epidemiol Biomarkers Prev
Pays: United States
ID NLM: 9200608

Informations de publication

Date de publication:
06 03 2023
Historique:
received: 06 07 2022
revised: 19 09 2022
accepted: 19 12 2022
pubmed: 29 12 2022
medline: 8 3 2023
entrez: 28 12 2022
Statut: ppublish

Résumé

Tobacco smoking is an established risk factor for colorectal cancer. However, genetically defined population subgroups may have increased susceptibility to smoking-related effects on colorectal cancer. A genome-wide interaction scan was performed including 33,756 colorectal cancer cases and 44,346 controls from three genetic consortia. Evidence of an interaction was observed between smoking status (ever vs. never smokers) and a locus on 3p12.1 (rs9880919, P = 4.58 × 10-8), with higher associated risk in subjects carrying the GG genotype [OR, 1.25; 95% confidence interval (CI), 1.20-1.30] compared with the other genotypes (OR <1.17 for GA and AA). Among ever smokers, we observed interactions between smoking intensity (increase in 10 cigarettes smoked per day) and two loci on 6p21.33 (rs4151657, P = 1.72 × 10-8) and 8q24.23 (rs7005722, P = 2.88 × 10-8). Subjects carrying the rs4151657 TT genotype showed higher risk (OR, 1.12; 95% CI, 1.09-1.16) compared with the other genotypes (OR <1.06 for TC and CC). Similarly, higher risk was observed among subjects carrying the rs7005722 AA genotype (OR, 1.17; 95% CI, 1.07-1.28) compared with the other genotypes (OR <1.13 for AC and CC). Functional annotation revealed that SNPs in 3p12.1 and 6p21.33 loci were located in regulatory regions, and were associated with expression levels of nearby genes. Genetic models predicting gene expression revealed that smoking parameters were associated with lower colorectal cancer risk with higher expression levels of CADM2 (3p12.1) and ATF6B (6p21.33). Our study identified novel genetic loci that may modulate the risk for colorectal cancer of smoking status and intensity, linked to tumor suppression and immune response. These findings can guide potential prevention treatments.

Sections du résumé

BACKGROUND
Tobacco smoking is an established risk factor for colorectal cancer. However, genetically defined population subgroups may have increased susceptibility to smoking-related effects on colorectal cancer.
METHODS
A genome-wide interaction scan was performed including 33,756 colorectal cancer cases and 44,346 controls from three genetic consortia.
RESULTS
Evidence of an interaction was observed between smoking status (ever vs. never smokers) and a locus on 3p12.1 (rs9880919, P = 4.58 × 10-8), with higher associated risk in subjects carrying the GG genotype [OR, 1.25; 95% confidence interval (CI), 1.20-1.30] compared with the other genotypes (OR <1.17 for GA and AA). Among ever smokers, we observed interactions between smoking intensity (increase in 10 cigarettes smoked per day) and two loci on 6p21.33 (rs4151657, P = 1.72 × 10-8) and 8q24.23 (rs7005722, P = 2.88 × 10-8). Subjects carrying the rs4151657 TT genotype showed higher risk (OR, 1.12; 95% CI, 1.09-1.16) compared with the other genotypes (OR <1.06 for TC and CC). Similarly, higher risk was observed among subjects carrying the rs7005722 AA genotype (OR, 1.17; 95% CI, 1.07-1.28) compared with the other genotypes (OR <1.13 for AC and CC). Functional annotation revealed that SNPs in 3p12.1 and 6p21.33 loci were located in regulatory regions, and were associated with expression levels of nearby genes. Genetic models predicting gene expression revealed that smoking parameters were associated with lower colorectal cancer risk with higher expression levels of CADM2 (3p12.1) and ATF6B (6p21.33).
CONCLUSIONS
Our study identified novel genetic loci that may modulate the risk for colorectal cancer of smoking status and intensity, linked to tumor suppression and immune response.
IMPACT
These findings can guide potential prevention treatments.

Identifiants

pubmed: 36576985
pii: 712418
doi: 10.1158/1055-9965.EPI-22-0763
pmc: PMC9992283
mid: NIHMS1861189
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

315-328

Subventions

Organisme : NHGRI NIH HHS
ID : U01 HG004438
Pays : United States
Organisme : NHGRI NIH HHS
ID : U01 HG004446
Pays : United States
Organisme : NCI NIH HHS
ID : K05 CA154337
Pays : United States
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Organisme : Cancer Research UK
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Pays : United Kingdom
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Informations de copyright

©2022 American Association for Cancer Research.

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Auteurs

Robert Carreras-Torres (R)

Colorectal Cancer Group, ONCOBELL Program, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.
Oncology Data Analytics Program, Catalan Institute of Oncology, L'Hospitalet de Llobregat, Barcelona, Spain.
Digestive Diseases and Microbiota Group, Girona Biomedical Research Institute (IDIBGI), Salt, Girona, Spain.

Andre E Kim (AE)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Yi Lin (Y)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Virginia Díez-Obrero (V)

Colorectal Cancer Group, ONCOBELL Program, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.
Oncology Data Analytics Program, Catalan Institute of Oncology, L'Hospitalet de Llobregat, Barcelona, Spain.
Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain.

Stephanie A Bien (SA)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Conghui Qu (C)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Jun Wang (J)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Niki Dimou (N)

Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France.

Elom K Aglago (EK)

Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France.

Demetrius Albanes (D)

Division of Cancer Epidemiology and Genetics, NCI, NIH, Bethesda, Maryland.

Volker Arndt (V)

Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ), Heidelberg, Germany.

James W Baurley (JW)

Bioinformatics and Data Science Research Center, Bina Nusantara University, Jakarta, Indonesia.

Sonja I Berndt (SI)

Division of Cancer Epidemiology and Genetics, NCI, NIH, Bethesda, Maryland.

Stéphane Bézieau (S)

Service de Génétique Médicale, Centre Hospitalier Universitaire (CHU) Nantes, Nantes, France.

D Timothy Bishop (DT)

Leeds Institute of Cancer and Pathology, University of Leeds, Leeds, United Kingdom.

Emmanouil Bouras (E)

Department of Hygiene and Epidemiology, University of Ioannina School of Medicine, Ioannina, Greece.

Hermann Brenner (H)

Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Division of Preventive Oncology, German Cancer Research Center (DKFZ) and National Center for Tumor Diseases (NCT), Heidelberg, Germany.
German Cancer Consortium (DKTK), German Cancer Research Center (DKFZ), Heidelberg, Germany.

Arif Budiarto (A)

Bioinformatics and Data Science Research Center, Bina Nusantara University, Jakarta, Indonesia.

Peter T Campbell (PT)

Behavioral and Epidemiology Research Group, American Cancer Society, Atlanta, Georgia.

Graham Casey (G)

Center for Public Health Genomics, Department of Public Health Sciences, University of Virginia, Charlottesville, Virginia.

Andrew T Chan (AT)

Division of Gastroenterology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.

Jenny Chang-Claude (J)

Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Xuechen Chen (X)

Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ), Heidelberg, Germany.

David V Conti (DV)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Christopher H Dampier (CH)

Department of General Surgery, University of Virginia School of Medicine, Charlottesville, Virginia.

Matthew A M Devall (MAM)

Center for Public Health Genomics, Department of Public Health Sciences, University of Virginia, Charlottesville, Virginia.

David A Drew (DA)

Clinical & Translational Epidemiology Unit, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts.

Jane C Figueiredo (JC)

Department of Medicine, Samuel Oschin Comprehensive Cancer Institute, Cedars-Sinai Medical Center, Los Angeles, California.

Steven Gallinger (S)

Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada.

Graham G Giles (GG)

Cancer Epidemiology Division, Cancer Council Victoria, Melbourne, Victoria, Australia.

Stephen B Gruber (SB)

Department of Medical Oncology & Therapeutics Research, City of Hope National Medical Center, Duarte, California.

Andrea Gsur (A)

Institute of Cancer Research, Department of Medicine I, Medical University Vienna, Vienna, Austria.

Marc J Gunter (MJ)

Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France.

Tabitha A Harrison (TA)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Akihisa Hidaka (A)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Michael Hoffmeister (M)

Division of Clinical Epidemiology and Aging Research, German Cancer Research Center (DKFZ), Heidelberg, Germany.

Jeroen R Huyghe (JR)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Mark A Jenkins (MA)

Centre for Epidemiology and Biostatistics, Melbourne School of Population and Global Health, The University of Melbourne, Melbourne, Victoria, Australia.

Kristina M Jordahl (KM)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Eric Kawaguchi (E)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Temitope O Keku (TO)

Center for Gastrointestinal Biology and Disease, University of North Carolina, Chapel Hill, North Carolina.

Anshul Kundaje (A)

Department of Genetics, Department of Computer Science, Stanford University, Stanford, California.

Loic Le Marchand (L)

University of Hawaii Cancer Center, Honolulu, Hawaii.

Juan Pablo Lewinger (JP)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Li Li (L)

Department of Family Medicine, University of Virginia, Charlottesville, Virginia.

Bharuno Mahesworo (B)

Bioinformatics and Data Science Research Center, Bina Nusantara University, Jakarta, Indonesia.

John L Morrison (JL)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Neil Murphy (N)

Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France.

Hongmei Nan (H)

Department of Epidemiology, Richard M. Fairbanks School of Public Health, Indianapolis, Indiana.

Rami Nassir (R)

Department of Pathology, School of Medicine, Umm Al-Qura'a University, Saudi Arabia.

Polly A Newcomb (PA)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Mireia Obón-Santacana (M)

Colorectal Cancer Group, ONCOBELL Program, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.
Oncology Data Analytics Program, Catalan Institute of Oncology, L'Hospitalet de Llobregat, Barcelona, Spain.
Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain.

Shuji Ogino (S)

Program in MPE Molecular Pathological Epidemiology, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts.
Department of Oncologic Pathology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, Massachusetts.
Broad Institute of MIT and Harvard, Cambridge, Massachusetts.

Jennifer Ose (J)

Huntsman Cancer Institute, Salt Lake City, Utah.
Department of Population Health Sciences, University of Utah, Salt Lake City, Utah.

Rish K Pai (RK)

Department of Laboratory Medicine and Pathology, Mayo Clinic Arizona, Scottsdale, Arizona.

Julie R Palmer (JR)

Slone Epidemiology Center at Boston University, Boston, Massachusetts.

Nikos Papadimitriou (N)

Nutrition and Metabolism Branch, International Agency for Research on Cancer, Lyon, France.

Bens Pardamean (B)

Bioinformatics and Data Science Research Center, Bina Nusantara University, Jakarta, Indonesia.

Anita R Peoples (AR)

Huntsman Cancer Institute, Salt Lake City, Utah.

Paul D P Pharoah (PDP)

Department of Public Health and Primary Care, University of Cambridge, Cambridge, United Kingdom.

Elizabeth A Platz (EA)

Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Gad Rennert (G)

Department of Community Medicine and Epidemiology, Lady Davis Carmel Medical Center, Haifa, Israel.

Edward Ruiz-Narvaez (E)

Department of Nutritional Sciences, University of Michigan School of Public Health, Ann Arbor, Michigan.

Lori C Sakoda (LC)

Division of Research, Kaiser Permanente Northern California, Oakland, California.

Peter C Scacheri (PC)

Department of Genetics and Genome Sciences, Case Western Reserve University, Cleveland, Ohio.

Stephanie L Schmit (SL)

Genomic Medicine Institute, Cleveland Clinic, Cleveland, Ohio.
Population and Cancer Prevention Program, Case Comprehensive Cancer Center, Cleveland, Ohio.

Robert E Schoen (RE)

Department of Medicine and Epidemiology, University of Pittsburgh Medical Center, Pittsburgh, Pennsylvania.

Anna Shcherbina (A)

Biomedical Informatics Program, Dept. of Biomedical Data Sciences, Stanford University, Stanford, California.

Martha L Slattery (ML)

Department of Internal Medicine, University of Utah, Salt Lake City, Utah.

Mariana C Stern (MC)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Yu-Ru Su (YR)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Catherine M Tangen (CM)

SWOG Statistical Center, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Duncan C Thomas (DC)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

Yu Tian (Y)

Division of Cancer Epidemiology, German Cancer Research Center (DKFZ), Heidelberg, Germany.
School of Public Health, Capital Medical University, Beijing, P.R. China.

Konstantinos K Tsilidis (KK)

Department of Epidemiology and Biostatistics, School of Public Health, Imperial College London, London, United Kingdom.
Department of Hygiene and Epidemiology, University of Ioannina School of Medicine, Ioannina, Greece.

Cornelia M Ulrich (CM)

Huntsman Cancer Institute, Salt Lake City, Utah.
Department of Population Health Sciences, University of Utah, Salt Lake City, Utah.

Fränzel J B van Duijnhoven (FJB)

Division of Human Nutrition and Health, Wageningen University & Research, Wageningen, the Netherlands.

Bethany Van Guelpen (B)

Department of Radiation Sciences, Oncology Unit, Umeå University, Umeå, Sweden.
Wallenberg Centre for Molecular Medicine, Umeå University, Umeå, Sweden.

Kala Visvanathan (K)

Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland.

Pavel Vodicka (P)

Department of Molecular Biology of Cancer, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, and Biomedical Center, Medical Faculty, Pilsen, Czech Republic.

Tjeng Wawan Cenggoro (TW)

Bioinformatics and Data Science Research Center, Bina Nusantara University, Jakarta, Indonesia.

Stephanie J Weinstein (SJ)

Division of Cancer Epidemiology and Genetics, NCI, NIH, Bethesda, Maryland.

Emily White (E)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Alicja Wolk (A)

Institute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden.

Michael O Woods (MO)

Memorial University of Newfoundland, Discipline of Genetics, St. John's, Canada.

Li Hsu (L)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.

Ulrike Peters (U)

Public Health Sciences Division, Fred Hutchinson Cancer Research Center, Seattle, Washington.
School of Public Health, University of Washington, Seattle, Washington.

Victor Moreno (V)

Colorectal Cancer Group, ONCOBELL Program, Bellvitge Biomedical Research Institute (IDIBELL), L'Hospitalet de Llobregat, Barcelona, Spain.
Oncology Data Analytics Program, Catalan Institute of Oncology, L'Hospitalet de Llobregat, Barcelona, Spain.
Consortium for Biomedical Research in Epidemiology and Public Health (CIBERESP), Madrid, Spain.
Department of Clinical Sciences, Faculty of Medicine, University of Barcelona, Barcelona, Spain.

W James Gauderman (WJ)

Division of Biostatistics, Department of Population and Public Health Sciences, Keck School of Medicine, University of Southern California, Los Angeles, California.

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