Circulating Small Noncoding RNA Profiling as a Potential Biomarker of Atherosclerotic Plaque Composition in Type 1 Diabetes.
Journal
Diabetes care
ISSN: 1935-5548
Titre abrégé: Diabetes Care
Pays: United States
ID NLM: 7805975
Informations de publication
Date de publication:
01 03 2023
01 03 2023
Historique:
received:
23
07
2022
accepted:
05
12
2022
pubmed:
29
12
2022
medline:
25
2
2023
entrez:
28
12
2022
Statut:
ppublish
Résumé
Cardiovascular disease (CVD) accounts for most deaths in patients with type 1 diabetes (T1D); however, the determinants of plaque composition are unknown. miRNAs regulate gene expression, participate in the development of atherosclerosis, and represent promising CVD biomarkers. This study analyzed the circulating miRNA expression profile in T1D with either carotid calcified (CCP) or fibrous plaque (CFP). Circulating small noncoding RNAs were sequenced and quantified using next-generation sequencing and bioinformatic analysis in an exploratory set of 26 subjects with T1D with CCP and in 25 with CFP. Then, in a validation set of 40 subjects with CCP, 40 with CFP, and 24 control subjects with T1D, selected miRNA expression was measured by digital droplet PCR. Putative gene targets enriched for pathways implicated in atherosclerosis/vascular calcification/diabetes were analyzed. The patients' main clinical characteristics were also recorded. miR-503-5p, let-7d-5p, miR-106b-3p, and miR-93-5p were significantly upregulated, while miR-10a-5p was downregulated in patients with CCP compared with CFP (all fold change >±1.5; P < 0.05). All candidate miRNAs showed a significant correlation with LDL-cholesterol, direct for the upregulated and inverse for the downregulated miRNA, in CCP. Many target genes of upregulated miRNAs in CCP participate in osteogenic differentiation, apoptosis, inflammation, cholesterol metabolism, and extracellular matrix organization. These findings characterize miRNAs and their signature in the regulatory network of carotid plaque phenotype in T1D, providing new insights into plaque pathophysiology and possibly novel biomarkers of plaque composition.
Identifiants
pubmed: 36577032
pii: 148229
doi: 10.2337/dc22-1441
pmc: PMC10020028
doi:
Substances chimiques
RNA, Small Untranslated
0
MicroRNAs
0
Biomarkers
0
Cholesterol
97C5T2UQ7J
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
551-560Informations de copyright
© 2023 by the American Diabetes Association.
Références
Arterioscler Thromb Vasc Biol. 2017 Feb;37(2):191-204
pubmed: 27908890
Lipids Health Dis. 2016 Mar 09;15:51
pubmed: 26956882
Cardiovasc Diabetol. 2015 Mar 18;14:33
pubmed: 25856787
Front Cell Dev Biol. 2021 Jun 09;9:668648
pubmed: 34178993
Diabetes Care. 2015 Oct;38(10):1937-44
pubmed: 26253729
Cardiovasc Diabetol. 2014 Dec 12;13:160
pubmed: 25496604
Sci Rep. 2016 Jan 29;6:20059
pubmed: 26821827
Front Physiol. 2020 Jul 07;11:793
pubmed: 32733281
Bioinformatics. 2016 Aug 15;32(16):2481-9
pubmed: 27153631
Diabetes. 2020 Mar;69(3):291-299
pubmed: 32079705
Arterioscler Thromb Vasc Biol. 2021 Apr;41(4):e224-e237
pubmed: 33626907
Cerebrovasc Dis. 2012;34(4):290-6
pubmed: 23128470
Nucleic Acids Res. 2019 Jan 8;47(D1):D155-D162
pubmed: 30423142
Nucleic Acids Res. 2019 Jan 8;47(D1):D175-D180
pubmed: 30371818
Circulation. 2011 Jan 25;123(3):282-91
pubmed: 21220732
J Am Coll Cardiol. 2012 May 1;59(18):1592-7
pubmed: 22538329
J Bone Miner Res. 2014 Feb;29(2):338-47
pubmed: 23821519
Circulation. 1990 Oct;82(4):1230-42
pubmed: 2205416
Circ Res. 2013 Jul 19;113(3):252-65
pubmed: 23748430
Diabetologia. 2022 Sep;65(9):1555-1568
pubmed: 35708762
PLoS One. 2013 Dec 05;8(12):e80738
pubmed: 24339880
JACC Cardiovasc Imaging. 2013 Nov;6(11):1160-7
pubmed: 24229768
BMC Endocr Disord. 2019 Apr 29;19(1):42
pubmed: 31035988
Cardiovasc Endocrinol Metab. 2019 Feb 13;8(1):14-27
pubmed: 31646294
Atherosclerosis. 2017 Jan;256:29-34
pubmed: 27998824
J Clin Med. 2019 Mar 28;8(4):
pubmed: 30925670
Pharmacol Ther. 2021 Feb;218:107675
pubmed: 32910935
Biomol Detect Quantif. 2017 Dec 18;15:1-5
pubmed: 29276692
Circ Res. 2013 Mar 29;112(7):1073-84
pubmed: 23538277
Mol Med Rep. 2020 Jul;22(1):135-144
pubmed: 32377690
Cardiovasc Diabetol. 2019 May 4;18(1):58
pubmed: 31054573
Circ Cardiovasc Imaging. 2021 Apr;14(4):e011701
pubmed: 33827231
Diabetes Care. 2013 Sep;36(9):2607-14
pubmed: 23835686
Ann N Y Acad Sci. 2021 Jul;1495(1):55-77
pubmed: 33521946
Arterioscler Thromb Vasc Biol. 2021 Jul;41(7):2149-2167
pubmed: 33980036
Cardiovasc Res. 2017 Nov 01;113(13):1627-1638
pubmed: 29016810
Diabetes Care. 2011 Apr;34(4):968-74
pubmed: 21447665
Exp Neurol. 2012 Jun;235(2):476-83
pubmed: 22119192
Proc Natl Acad Sci U S A. 2010 Jul 27;107(30):13450-5
pubmed: 20624982
Stroke. 2007 Jul;38(7):2074-8
pubmed: 17525393