Tissue fluidification promotes a cGAS-STING cytosolic DNA response in invasive breast cancer.


Journal

Nature materials
ISSN: 1476-4660
Titre abrégé: Nat Mater
Pays: England
ID NLM: 101155473

Informations de publication

Date de publication:
05 2023
Historique:
received: 25 08 2021
accepted: 02 11 2022
medline: 5 5 2023
pubmed: 30 12 2022
entrez: 29 12 2022
Statut: ppublish

Résumé

The process in which locally confined epithelial malignancies progressively evolve into invasive cancers is often promoted by unjamming, a phase transition from a solid-like to a liquid-like state, which occurs in various tissues. Whether this tissue-level mechanical transition impacts phenotypes during carcinoma progression remains unclear. Here we report that the large fluctuations in cell density that accompany unjamming result in repeated mechanical deformations of cells and nuclei. This triggers a cellular mechano-protective mechanism involving an increase in nuclear size and rigidity, heterochromatin redistribution and remodelling of the perinuclear actin architecture into actin rings. The chronic strains and stresses associated with unjamming together with the reduction of Lamin B1 levels eventually result in DNA damage and nuclear envelope ruptures, with the release of cytosolic DNA that activates a cGAS-STING (cyclic GMP-AMP synthase-signalling adaptor stimulator of interferon genes)-dependent cytosolic DNA response gene program. This mechanically driven transcriptional rewiring ultimately alters the cell state, with the emergence of malignant traits, including epithelial-to-mesenchymal plasticity phenotypes and chemoresistance in invasive breast carcinoma.

Identifiants

pubmed: 36581770
doi: 10.1038/s41563-022-01431-x
pii: 10.1038/s41563-022-01431-x
pmc: PMC10156599
doi:

Substances chimiques

Actins 0
DNA 9007-49-2
Nucleotidyltransferases EC 2.7.7.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

644-655

Commentaires et corrections

Type : ErratumIn
Type : CommentIn

Informations de copyright

© 2022. The Author(s).

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Auteurs

Emanuela Frittoli (E)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Andrea Palamidessi (A)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy. andrea.palamidessi@ifom.eu.

Fabio Iannelli (F)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Federica Zanardi (F)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Stefano Villa (S)

Department of Medical Biotechnology and Translational Medicine, University of Milan, Segrate, Italy.
Max Plank Institute for Dynamics and Self-Organization, Göttingen, Germany.

Leonardo Barzaghi (L)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Hind Abdo (H)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Valeria Cancila (V)

Department of Health Sciences, Human Pathology Section, University of Palermo School of Medicine, Palermo, Italy.

Galina V Beznoussenko (GV)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Giulia Della Chiara (G)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Massimiliano Pagani (M)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.
Department of Medical Biotechnology and Translational Medicine, University of Milan, Segrate, Italy.

Chiara Malinverno (C)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Dipanjan Bhattacharya (D)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Federica Pisati (F)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Weimiao Yu (W)

Institute of Molecular and Cell Biology, A*STAR, Singapore, & Bioinformatics Institute, A*STAR, Singapore, Singapore.

Viviana Galimberti (V)

European Institute of Oncology (IEO) IRCCS, Milan, Italy.

Giuseppina Bonizzi (G)

European Institute of Oncology (IEO) IRCCS, Milan, Italy.

Emanuele Martini (E)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Alexander A Mironov (AA)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Ubaldo Gioia (U)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Flora Ascione (F)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Qingsen Li (Q)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Kristina Havas (K)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Serena Magni (S)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Zeno Lavagnino (Z)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Fabrizio Andrea Pennacchio (FA)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Paolo Maiuri (P)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.
Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Naples, Italy.

Silvia Caponi (S)

Istituto Officina dei Materiali, National Research Council (IOM-CNR), Unit of Perugia, c/o Department of Physics and Geology, University of Perugia, Perugia, Italy.

Maurizio Mattarelli (M)

Department of Physics and Geology, University of Perugia, Perugia, Italy.

Sabata Martino (S)

Department of Chemistry, Biology and Biotechnology, Biochemical and Biotechnological Sciences, University of Perugia, Perugia, Italy.

Fabrizio d'Adda di Fagagna (F)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.
Institute of Molecular Genetics, National Research Council, Pavia, Italy.

Chiara Rossi (C)

Unit of Anatomic Pathology, Department of Molecular Medicine, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy.

Marco Lucioni (M)

Unit of Anatomic Pathology, Department of Molecular Medicine, Fondazione IRCCS Policlinico San Matteo, University of Pavia, Pavia, Italy.

Richard Tancredi (R)

Medical Oncology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
S.C. Oncologia Medica, ASST Melegnano e della Martesana, Ospedale Uboldo, Cernusco sul Naviglio, Milan, Italy.

Paolo Pedrazzoli (P)

Medical Oncology Unit, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy.
Department of Internal Medicine and Medical Therapy, University of Pavia, Pavia, Italy.

Andrea Vecchione (A)

Department of Clinical and Molecular Medicine, University of Roma, La Sapienza, Rome, Italy.

Cristiano Petrini (C)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.

Francesco Ferrari (F)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.
Institute of Molecular Genetics, National Research Council, Pavia, Italy.

Chiara Lanzuolo (C)

Institute of Biomedical Technologies, National Research Council, Milan, Italy.
National Institute of Molecular Genetics Romeo and Enrica Invernizzi, INGM, Milan, Italy.

Giovanni Bertalot (G)

Department of Pathology, S. Chiara Hospital, Azienda Provinciale per i Servizi Sanitari, Trento, Italy.
CISMed University of Trento, University of Trento, Trento, Italy.

Guilherme Nader (G)

Institut Curie and Institut Pierre Gilles de Gennes, PSL Research University, CNRS, UMR-144, Paris, France.
Cell Pathology Children's Hospital of Philadelphia, Research Institute Department of Pathology and Laboratory Medicine University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.

Marco Foiani (M)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy.
Department of Oncology and Haemato-Oncology, University of Milan, Milan, Italy.

Matthieu Piel (M)

Institut Curie and Institut Pierre Gilles de Gennes, PSL Research University, CNRS, UMR-144, Paris, France.

Roberto Cerbino (R)

Department of Medical Biotechnology and Translational Medicine, University of Milan, Segrate, Italy.
Faculty of Physics, University of Vienna, Vienna, Austria.

Fabio Giavazzi (F)

Department of Medical Biotechnology and Translational Medicine, University of Milan, Segrate, Italy. fabio.giavazzi@unimi.it.

Claudio Tripodo (C)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy. claudio.tripodo@unipa.it.
Department of Health Sciences, Human Pathology Section, University of Palermo School of Medicine, Palermo, Italy. claudio.tripodo@unipa.it.

Giorgio Scita (G)

IFOM, the FIRC Institute of Molecular Oncology, Milan, Italy. giorgio.scita@ifom.eu.
Department of Oncology and Haemato-Oncology, University of Milan, Milan, Italy. giorgio.scita@ifom.eu.

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Classifications MeSH