Structural Analyses of the Glycolipids in Lipid Rafts.
1,2-dichloroethane
Detergent removal
Lipid raft
Mass spectrometry
Membrane microdomain
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2023
2023
Historique:
entrez:
31
12
2022
pubmed:
1
1
2023
medline:
4
1
2023
Statut:
ppublish
Résumé
Lipid rafts are usually isolated from cells or tissues using sucrose gradient ultracentrifugation in the presence of detergents such as Triton X-100 at 4 °C. Although detergents should be removed for further structural characterization following fractionation, these compounds are often difficult to completely remove, especially from the glycolipids. In this chapter, we describe a novel method for the fast and convenient removal of detergents from lipid raft glycolipids following fraction and describe the application of this method.
Identifiants
pubmed: 36587077
doi: 10.1007/978-1-0716-2910-9_12
doi:
Substances chimiques
Glycolipids
0
Detergents
0
Octoxynol
9002-93-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
145-152Informations de copyright
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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