Serum Calcitonin Gene-Related Peptide α and β Levels are Increased in COVID-19 Inpatients.


Journal

Archives of medical research
ISSN: 1873-5487
Titre abrégé: Arch Med Res
Pays: United States
ID NLM: 9312706

Informations de publication

Date de publication:
01 2023
Historique:
received: 04 07 2022
revised: 25 10 2022
accepted: 05 12 2022
pubmed: 2 1 2023
medline: 28 1 2023
entrez: 1 1 2023
Statut: ppublish

Résumé

Vasoactive peptides play an important role in a wide range of physiological and pathological conditions. Due to its known functions, the calcitonin gene-related peptide (CGRP) has been suggested as a possible modulator of the hyperimmune response in COVID-19 and thus, blocking its action may lessen the pulmonary effects of COVID-19. To compare the circulating levels of CGRPα and CGRPβ in healthy controls compared to hospitalized COVID-19 patients. The study also analyzed how different comorbidities and treatments may affect these concentrations in cases of COVID-19 infection with pulmonary involvement METHODS: Serum samples were collected from the antecubital vein of 51 control subjects (mean age = 55 ± 14 years; range = 26-77; 56.9% female) and 52 patients hospitalized with COVID-19 infection (mean age = 55 ± 13; range = 23-77; 55.8% female) from December 2020 to May 2021. Enzyme-linked immunosorbent assays (ELISAs) were used for CGRPα (Abbexa, UK) and CGRPβ (CUSABIO, China) measurements. Comorbidities, symptoms, and treatments of infection were listed. The results showed that the serum levels of both isoforms of CGRP were significantly higher in patients with COVID-19 (α: 57.9 ± 35.8 pg/mL; β: 6.1 ± 2.6 pg/mL) compared to controls (α: 41.8 ± 25.4 pg/mL; β: 4.5 ± 2.4 pg/mL) (p <0.01). Also, the presence of arterial hypertension (HT), obesity, or corticosteroid treatment significantly alter the serum concentration of CGRPα in the subgroups compared to controls. The elevated serum CGRP levels found in our COVID-19 group compared to controls may suggest that CGRP plays a role in the pathophysiology of the disease, more specifically, in the cytokine storm and in the pulmonary involvement. Future studies should focus on the source of this CGRP elevation.

Sections du résumé

BACKGROUND
Vasoactive peptides play an important role in a wide range of physiological and pathological conditions. Due to its known functions, the calcitonin gene-related peptide (CGRP) has been suggested as a possible modulator of the hyperimmune response in COVID-19 and thus, blocking its action may lessen the pulmonary effects of COVID-19.
AIM OF THE STUDY
To compare the circulating levels of CGRPα and CGRPβ in healthy controls compared to hospitalized COVID-19 patients. The study also analyzed how different comorbidities and treatments may affect these concentrations in cases of COVID-19 infection with pulmonary involvement METHODS: Serum samples were collected from the antecubital vein of 51 control subjects (mean age = 55 ± 14 years; range = 26-77; 56.9% female) and 52 patients hospitalized with COVID-19 infection (mean age = 55 ± 13; range = 23-77; 55.8% female) from December 2020 to May 2021. Enzyme-linked immunosorbent assays (ELISAs) were used for CGRPα (Abbexa, UK) and CGRPβ (CUSABIO, China) measurements. Comorbidities, symptoms, and treatments of infection were listed.
RESULTS
The results showed that the serum levels of both isoforms of CGRP were significantly higher in patients with COVID-19 (α: 57.9 ± 35.8 pg/mL; β: 6.1 ± 2.6 pg/mL) compared to controls (α: 41.8 ± 25.4 pg/mL; β: 4.5 ± 2.4 pg/mL) (p <0.01). Also, the presence of arterial hypertension (HT), obesity, or corticosteroid treatment significantly alter the serum concentration of CGRPα in the subgroups compared to controls.
CONCLUSION
The elevated serum CGRP levels found in our COVID-19 group compared to controls may suggest that CGRP plays a role in the pathophysiology of the disease, more specifically, in the cytokine storm and in the pulmonary involvement. Future studies should focus on the source of this CGRP elevation.

Identifiants

pubmed: 36588002
pii: S0188-4409(22)00178-3
doi: 10.1016/j.arcmed.2022.12.002
pmc: PMC9801185
pii:
doi:

Substances chimiques

Calcitonin Gene-Related Peptide JHB2QIZ69Z

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

56-63

Informations de copyright

Copyright © 2022. Published by Elsevier Inc.

Auteurs

Gabriel Gárate (G)

Service of Neurology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Marta Pascual (M)

Service of Gastroenterology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Montserrat Rivero (M)

Service of Gastroenterology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

María Toriello (M)

Service of Neurology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Sara Pérez-Pereda (S)

Service of Neurology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Vicente González-Quintanilla (V)

Service of Neurology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Jorge Madera (J)

Service of Neurology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Manuel Gutiérrez-Cuadra (M)

Service of Infectious Diseases, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

María Del Carmen Fariñas (MDC)

Service of Infectious Diseases, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

José Luis Hernández (JL)

Service of Internal Medicine, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

José Manuel Olmos (JM)

Service of Internal Medicine, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain.

Julio Pascual (J)

Service of Neurology, University Hospital Marqués de Valdecilla, Universidad de Cantabria and IDIVAL, Santander, Spain. Electronic address: juliopascualgomez@gmail.com.

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Classifications MeSH