Livestock-associated methicillin-resistant

LA-MRSA CC398 MRSA Staphylococcal infection/epidemiology Staphylococcus aureus antimicrobial surveillance cgMLST clustering one-health whole-genome sequencing

Journal

Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977

Informations de publication

Date de publication:
2022
Historique:
received: 14 02 2022
accepted: 08 11 2022
entrez: 2 1 2023
pubmed: 3 1 2023
medline: 3 1 2023
Statut: epublish

Résumé

While Livestock-associated methicillin-resistant In this retrospective study, we evaluated LA-MRSA strains that were collected between 2014 and 2018 from patients who received clinical care in a single urban area in Netherlands. Patient files were assessed for livestock exposure data, clinical findings, and contact tracing information. Next-generation sequencing (NGS) analysis in combination with wgMLST was conducted to assess genetic diversity and relatedness and to detect virulence and resistance genes. LA-MRSA strains were cultured from 81 patients, comprising 12% of all the MRSA strains found in seven study laboratories between 2014 and 2018. No livestock link was found in 76% of patients ( LA-MRSA may cause a relevant burden of disease in urban areas. Surprisingly, most infections in the present study occurred in the absence of a livestock link, suggesting inter-human transmission. These findings and the presence of PVL and other immune evasive complex virulence genes warrant future surveillance and preventative measures.

Identifiants

pubmed: 36590396
doi: 10.3389/fmicb.2022.875775
pmc: PMC9795226
doi:

Types de publication

Journal Article

Langues

eng

Pagination

875775

Informations de copyright

Copyright © 2022 Konstantinovski, Schouls, Witteveen, Claas, Kraakman, Kalpoe, Mattson, Hetem, van Elzakker, Kerremans, Hira, Bosch and Gooskens.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Références

Front Microbiol. 2021 Mar 04;12:636788
pubmed: 33746929
Clin Infect Dis. 2017 Oct 1;65(7):1072-1076
pubmed: 28575216
J Clin Microbiol. 2016 Dec;54(12):2874-2881
pubmed: 27558178
Clin Microbiol Infect. 2014 Oct;20(10):O764-71
pubmed: 24494859
mBio. 2018 Nov 13;9(6):
pubmed: 30425152
Euro Surveill. 2019 Oct;24(42):
pubmed: 31640842
Euro Surveill. 2015;20(37):
pubmed: 26535590
J Clin Microbiol. 2014 Jul;52(7):2365-70
pubmed: 24759713
Clin Microbiol Infect. 2012 Jul;18(7):656-61
pubmed: 21967090
Appl Environ Microbiol. 2012 Dec;78(24):8845-8
pubmed: 23042163
J Clin Microbiol. 2015 Jun;53(6):1836-41
pubmed: 25809975
PLoS One. 2013;8(2):e55040
pubmed: 23418434
Epidemiol Infect. 2010 May;138(5):756-63
pubmed: 20141647
PLoS One. 2017 Jun 2;12(6):e0179003
pubmed: 28575112
Trends Microbiol. 2020 Jun;28(6):465-477
pubmed: 31948727
Epidemiol Infect. 2012 Oct;140(10):1800-8
pubmed: 22313681
PLoS One. 2009;4(4):e5082
pubmed: 19343175
Trends Microbiol. 2012 Apr;20(4):192-8
pubmed: 22386364
PLoS One. 2009 Aug 27;4(8):e6800
pubmed: 19710922
Sci Rep. 2019 Dec 9;9(1):18655
pubmed: 31819134
Clin Infect Dis. 2016 Dec 1;63(11):1431-1438
pubmed: 27516381
Euro Surveill. 2016 May 26;21(21):
pubmed: 27254022
Infection. 2013 Apr;41(2):339-46
pubmed: 22941568
Epidemiol Infect. 2009 May;137(5):700-8
pubmed: 18947444

Auteurs

Maria M Konstantinovski (MM)

Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands.
Department of Microbiology, Medical Laboratories, Reinier de Graaf Groep, Delft, Netherlands.

Leo M Schouls (LM)

Center for Infectious Diseases Research, Diagnostics and Laboratory Surveillance, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.

Sandra Witteveen (S)

Center for Infectious Diseases Research, Diagnostics and Laboratory Surveillance, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.

Eric C J Claas (ECJ)

Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands.

Margriet E Kraakman (ME)

Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands.

Jayant Kalpoe (J)

Department of Medical Microbiology, Regional Laboratory of Public Health Kennemerland, Haarlem, Netherlands.

Eva Mattson (E)

Department of Microbiology, Medical Laboratories, Reinier de Graaf Groep, Delft, Netherlands.

David J Hetem (DJ)

Department of Medical Microbiology, Haaglanden Medical Center, The Hague, Netherlands.

Erika P M van Elzakker (EPM)

Laboratory of Medical Microbiology, HagaZiekenhuis, The Hague, Netherlands.

Jos Kerremans (J)

Department of Medical Microbiology, Alrijne Hospital, Leiderdorp, Netherlands.

Vishal Hira (V)

Department of Medical Microbiology and Infection Prevention, Groene Hart Ziekenhuis, Gouda, Netherlands.

Thijs Bosch (T)

Center for Infectious Diseases Research, Diagnostics and Laboratory Surveillance, National Institute for Public Health and the Environment (RIVM), Bilthoven, Netherlands.

Jairo Gooskens (J)

Department of Medical Microbiology, Leiden University Medical Center, Leiden, Netherlands.

Classifications MeSH