Multi-OMICs landscape of SARS-CoV-2-induced host responses in human lung epithelial cells.
Metabolomics
Proteomics
Transcriptomics
Virology
Journal
iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038
Informations de publication
Date de publication:
20 Jan 2023
20 Jan 2023
Historique:
received:
09
09
2022
revised:
03
12
2022
accepted:
22
12
2022
pubmed:
3
1
2023
medline:
3
1
2023
entrez:
2
1
2023
Statut:
ppublish
Résumé
COVID-19 pandemic continues to remain a global health concern owing to the emergence of newer variants. Several multi-Omics studies have produced extensive evidence on host-pathogen interactions and potential therapeutic targets. Nonetheless, an increased understanding of host signaling networks regulated by post-translational modifications and their ensuing effect on the cellular dynamics is critical to expanding the current knowledge on SARS-CoV-2 infections. Through an unbiased transcriptomics, proteomics, acetylomics, phosphoproteomics, and exometabolome analysis of a lung-derived human cell line, we show that SARS-CoV-2 Norway/Trondheim-S15 strain induces time-dependent alterations in the induction of type I IFN response, activation of DNA damage response, dysregulated Hippo signaling, among others. We identified interplay of phosphorylation and acetylation dynamics on host proteins and its effect on the altered release of metabolites, especially organic acids and ketone bodies. Together, our findings serve as a resource of potential targets that can aid in designing novel host-directed therapeutic strategies.
Identifiants
pubmed: 36590899
doi: 10.1016/j.isci.2022.105895
pii: S2589-0042(22)02168-X
pmc: PMC9794516
doi:
Types de publication
Journal Article
Langues
eng
Pagination
105895Informations de copyright
© 2022 The Author(s).
Déclaration de conflit d'intérêts
The authors declare no competing interests.
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