Expression in Pichia pastoris of human antibody fragments that neutralize venoms of Mexican scorpions.
Heterologous expression
Human scFvs
Pichia pastoris
Scorpion venom neutralization
Journal
Toxicon : official journal of the International Society on Toxinology
ISSN: 1879-3150
Titre abrégé: Toxicon
Pays: England
ID NLM: 1307333
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
09
11
2022
revised:
16
12
2022
accepted:
27
12
2022
pubmed:
3
1
2023
medline:
28
1
2023
entrez:
2
1
2023
Statut:
ppublish
Résumé
The methylotrophic yeast Pichia pastoris has been one of the most widely used organisms in recent years as an expression system for a wide variety of recombinant proteins with therapeutic potential. Its popularity as an alternative system to Escherichia coli is mainly due to the easy genetic manipulation and the ability to produce high levels of heterologous proteins, either intracellularly or extracellularly. Being a eukaryotic organism, P. pastoris carries out post-translational modifications that allow it to produce soluble and correctly folded recombinant proteins. This work, evaluated the expression capacity in P. pastoris of two single-chain variable fragments (scFvs) of human origin, 10FG2 and LR. These scFvs were previously obtained by directed evolution against scorpion venom toxins and are able to neutralize different toxins and venoms of Mexican species. The yield obtained in P. pastoris was higher than that obtained in bacterial periplasm (E. coli), and most importantly, biochemical and functional properties were not modified. These results confirm that P. pastoris yeast can be a good expression system for the production of antibody fragments of a new recombinant antivenom.
Identifiants
pubmed: 36592762
pii: S0041-0101(22)00363-4
doi: 10.1016/j.toxicon.2022.107012
pii:
doi:
Substances chimiques
Venoms
0
Recombinant Proteins
0
Immunoglobulin Fragments
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107012Informations de copyright
Copyright © 2022 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Baltazar Becerril Lujan has patent COMPOSITION OF HUMAN RECOMBINANT ANTIBODY FRAGMENTS THAT COMPLETELY NEUTRALIZE THE VENOM OF THE SCORPION CENTRUROIDES SCULPTURATUS pending to 17/937,666.