Measuring ventral nerve cord stiffness in live flat-dissected Drosophila embryos by atomic force microscopy.
Atomic Force Microscopy (AFM)
Developmental biology
Model Organisms
Neuroscience
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
16 12 2022
16 12 2022
Historique:
received:
15
08
2022
revised:
06
10
2022
accepted:
10
11
2022
entrez:
3
1
2023
pubmed:
4
1
2023
medline:
6
1
2023
Statut:
ppublish
Résumé
Drosophila is an amenable system for addressing the mechanics of morphogenesis. We describe a workflow for characterizing the mechanical properties of its ventral nerve cord (VNC), at different developmental stages, in live, flat-dissected embryos employing atomic force microscopy (AFM). AFM is performed with spherical probes, and stiffness (Young's modulus) is calculated by fitting force curves with Hertz's contact model. For complete details on the use and execution of this protocol, please refer to Karkali et al. (2022).
Identifiants
pubmed: 36595903
pii: S2666-1667(22)00781-X
doi: 10.1016/j.xpro.2022.101901
pmc: PMC9732408
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101901Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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