A preliminary study on placental damage associated to experimental neosporosis in BALB/c mice.
Apoptosis
BALB/c mouse
Neospora caninum
Pathology
Placenta
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Mar 2023
Mar 2023
Historique:
received:
23
08
2022
accepted:
20
12
2022
pubmed:
4
1
2023
medline:
9
3
2023
entrez:
3
1
2023
Statut:
ppublish
Résumé
Neospora caninum is a protozoan parasite which can infect a range of animals, including dogs, cattle, and sheep. Bovine neosporosis, which mainly causes abortion in cattle, results in substantial economic losses worldwide. To study the effects of N. caninum infection on the placenta, a pregnant mouse model for N. caninum infection was established. The litter size (8.6 ± 1.5) and the number of live pups (6.4 ± 1.8) of infected dams were significantly lower compared with those of non-infected dams. Trophoblast cell shrinkage and a large number of apoptosomes were detected in the placentas of the infected group. The parasite load in the placental tissue was significantly higher with time after infection. Likewise, apoptosis of placental trophoblast cells significantly increased with time after infection. Among the 66 apoptotic genes detected in this study, eight genes, including Bcl-2, were significantly differentially expressed by about > tenfold in infected and uninfected mice. The expression of BAX and tumor necrosis factor-alpha (TNF-α) was upregulated in the placental cells of the infected mice, whereas the expression of BCL-2 was downregulated. Enzyme-linked immunosorbent assays (ELISAs) showed that apoptotic protease caspase-3 level was significantly increased in placental cell suspension, and insulin-like growth factor (IGF)-2 level was significantly reduced. Acetylcholine (ACH) and placental prolactin (PL) levels were initially decreased but eventually increased. In summary, infection of mice with N. caninum caused apoptotic damage to the placental tissues, cells, and genes and affected the normal physiological functions of placenta, which may largely explain the adverse pregnancy outcomes caused by N. caninum infection in mice.
Identifiants
pubmed: 36596902
doi: 10.1007/s00436-022-07771-6
pii: 10.1007/s00436-022-07771-6
doi:
Substances chimiques
Proto-Oncogene Proteins c-bcl-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
781-788Subventions
Organisme : the National Natural Science Foundation of China
ID : 32160839
Organisme : the Scientific Research and Innovation Team Project of Yanbian University, the Leading Talents and Teams of Young and Middle-aged Technological Innovation in Jilin Province
ID : 20200301034RQ
Organisme : the 111 Project
ID : D20034
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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