Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes.
developmental biology
hair cell
inner ear
neuroscience
supporting cell
zebrafish
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
04 01 2023
04 01 2023
Historique:
received:
25
08
2022
accepted:
04
01
2023
pubmed:
5
1
2023
medline:
24
1
2023
entrez:
4
1
2023
Statut:
epublish
Résumé
A major cause of human deafness and vestibular dysfunction is permanent loss of the mechanosensory hair cells of the inner ear. In non-mammalian vertebrates such as zebrafish, regeneration of missing hair cells can occur throughout life. While a comparative approach has the potential to reveal the basis of such differential regenerative ability, the degree to which the inner ears of fish and mammals share common hair cells and supporting cell types remains unresolved. Here, we perform single-cell RNA sequencing of the zebrafish inner ear at embryonic through adult stages to catalog the diversity of hair cells and non-sensory supporting cells. We identify a putative progenitor population for hair cells and supporting cells, as well as distinct hair and supporting cell types in the maculae versus cristae. The hair cell and supporting cell types differ from those described for the lateral line system, a distributed mechanosensory organ in zebrafish in which most studies of hair cell regeneration have been conducted. In the maculae, we identify two subtypes of hair cells that share gene expression with mammalian striolar or extrastriolar hair cells. In situ hybridization reveals that these hair cell subtypes occupy distinct spatial domains within the three macular organs, the utricle, saccule, and lagena, consistent with the reported distinct electrophysiological properties of hair cells within these domains. These findings suggest that primitive specialization of spatially distinct striolar and extrastriolar hair cells likely arose in the last common ancestor of fish and mammals. The similarities of inner ear cell type composition between fish and mammals validate zebrafish as a relevant model for understanding inner ear-specific hair cell function and regeneration.
Identifiants
pubmed: 36598134
doi: 10.7554/eLife.82978
pii: 82978
pmc: PMC9851615
doi:
pii:
Banques de données
GEO
['GSE211728', 'GSE144827', 'GSE152859', 'GSE196211', 'GSE155966', 'GSE168901', 'GSE123241', 'GSE178969']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDCR NIH HHS
ID : R35DE027550
Pays : United States
Organisme : NIDCR NIH HHS
ID : R35 DE027550
Pays : United States
Organisme : NIDCD NIH HHS
ID : R21 DC019948
Pays : United States
Organisme : NIDCD NIH HHS
ID : F31DC020898
Pays : United States
Organisme : NHGRI NIH HHS
ID : 1R01HG010632
Pays : United States
Organisme : NIDCD NIH HHS
ID : F31DC020633
Pays : United States
Organisme : NIDCD NIH HHS
ID : R21DC019948
Pays : United States
Organisme : NIDCD NIH HHS
ID : F31 DC020898
Pays : United States
Organisme : NIDCR NIH HHS
ID : K99 DE029858
Pays : United States
Organisme : NHGRI NIH HHS
ID : UM1 HG011586
Pays : United States
Organisme : NIDCD NIH HHS
ID : T32DC005361
Pays : United States
Organisme : NIDCD NIH HHS
ID : T32DC009975
Pays : United States
Organisme : NHGRI NIH HHS
ID : UM1HG011586
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01DC015829
Pays : United States
Informations de copyright
© 2023, Shi, Beaulieu et al.
Déclaration de conflit d'intérêts
TS, MB, LS, PF, CT, NS, JC, DR No competing interests declared
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