Morphological and molecular characterization of puparia of Piophilidae species of forensic relevance.
COI
DNA barcoding
cadaver colonization
forensic entomology
skipper flies
Journal
Medical and veterinary entomology
ISSN: 1365-2915
Titre abrégé: Med Vet Entomol
Pays: England
ID NLM: 8708682
Informations de publication
Date de publication:
06 2023
06 2023
Historique:
received:
15
08
2022
accepted:
08
12
2022
medline:
18
5
2023
pubmed:
5
1
2023
entrez:
4
1
2023
Statut:
ppublish
Résumé
Piophilidae are a small family of Diptera with a worldwide distribution and which are historically associated with human activities. In addition to their economic importance, piophilid larvae can also be of medical and legal relevance. Within a medicolegal context, piophilids are frequently associated with cadavers in advanced stages of decomposition, thus being potentially useful forensic indicators and they have been reported also from archaeo-funerary contexts. An accurate species identification is therefore an essential prerequisite to ensure the reliable analysis of insect material in medical, forensic and archaeological investigations. Identification of the adult piophilid flies is possible because of the availability of identification keys, in contrast immature insects, especially puparia, have been poorly investigated and described. In this paper, puparia of 11 species of forensic interest (Piophila casei, Piophila megastigmata, Parapiophila atrifrons, Parapiophila flavipes, Parapiophila vulgaris, Protopiophila litigata, Liopiophila varipes, Prochyliza nigrimana, Prochyliza xanthosoma and Stearibia nigriceps in subtribe Piophilina and Centrophlebomyia furcata in subtribe Thyreophorina) are described and a molecular analysis, based on the COI sequencing, is presented to show the potential of the molecular approach in their identification.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
339-358Informations de copyright
© 2023 Royal Entomological Society.
Références
Altschul, S.F., Gish, W., Miller, W., Myers, E.W. & Lipman, D.J. (1990) Basic local alignment search tool. Journal of Molecular Biology, 215, 403-410.
Boehme, P., Amendt, J. & Zehner, R. (2012) The use of COI barcodes for molecular identification of forensically important fly species in Germany. Parasitology Research, 110, 2325-2332.
Bonduriansky, R. (1995) A new Nearctic species of Protopiophila Duda (Diptera: Piophilidae), with notes on its behaviour and comparison with P. latipes (Meigen). Canadian Entomologist, 127, 859-863.
Braack, L.E.O. (1986) Arthropods associated with carcasscs in the northern Kruger National Park. South African Journal of Wildlife Research, 16, 91-98.
Candida, V.M. & Agatino, R. (2004) Piophila casei L.(Diptera: Piophilidae) monitoring in cheese ripening storehouses. IOBC/WPRS Bulletin Journal, 27, 109-114.
Cockburn, A., Barraco, R.A., Reyman, T.A. & Peck, W.H. (1975) Autopsy of an Egyptian mummy. Science, 187, 1155-1160.
Feng, D.-X. & Liu, G.-C. (2014) Pupal age estimation of forensically important Megaselia scalaris (Loew)(Diptera: Phoridae). Forensic Science International, 236, 133-137.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294-299.
Freidberg, A. (1981) Taxonomy, natural history and immature stages of the bone-skipper, Centrophlebomyia furcata (Fabricius)(Diptera: Piophilidae, Thyreophorina). Insect Systematics & Evolution, 12, 320-326.
Giordani, G., Grzywacz, A. & Vanin, S. (2019) Characterization and identification of puparia of Hydrotaea Robineau-Desvoidy, 1830 (Diptera: Muscidae) from forensic and archaeological contexts. Journal of Medical Entomology, 56, 45-54.
Giordani, G., Tuccia, F., Floris, I. & Vanin, S. (2018) First record of Phormia regina (Meigen, 1826) (Diptera: Calliphoridae) from mummies at the Sant'Antonio abate Cathedral of Castelsardo, Sardinia, Italy. PeerJ, 6, e4176.
Giordani, G. & Vanin, S. (2020) Description of the puparium and other notes on the morphological and molecular identification of Phthitia empirica (Diptera, Sphaeroceridae) collected from animal carcasses. Egyptian Journal of Forensic Sciences, 10, 13.
González Medina, A., Archilla Peña, F. & Jiménez Ríos, G. (2011) Las miasis como entidad de interés en Medicina del Trabajo. Medicina y Seguridad del Trabajo, 57, 331-338.
Grzywacz, A., Hall, M.J. & Pape, T. (2015) Morphology successfully separates third instar larvae of Muscina. Medical and Veterinary Entomology, 29, 314-329.
Grzywacz, A., Hall, M.J., Pape, T. & Szpila, K. (2017) Muscidae (Diptera) of forensic importance-an identification key to third instar larvae of the Western Palaearctic region and a catalogue of the muscid carrion community. International Journal of Legal Medicine, 131, 855-866.
Hebert, P.D., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society B: Biological Sciences, 270, 313-321.
Hennig, W. (1943) Piophilidae, pp. 1-53. In L. E (ed.), Die Fliegen der Palaearktischen Region, vol. 5. Schweizerbart, Stuttgart.
Hennig, W. (1955-1964) Muscidae, pp. 1110. In L. E. (ed.), Die Fliegen der Palaearktischen Region, vol. 7 (2). Schweizerbart, Stuttgart.
Huchet, J.B. (2014) Insect remains and their traces: relevant fossil witnesses in the reconstruction of past funerary practices. Anthropologie (Brno), 52, 329-346.
Huchet, J.B. & Greenberg, B. (2010) Flies, Mochicas and burial practices: A case study from Huaca de la Luna, Peru. Journal of Archaeological Science, 37, 2846-2856.
Kim, H., Martín-Vega, D., Shin, S.E., Wang, A.R. & Park, S.H. (2021) First report of the forensically important fly, Stearibia nigriceps (Diptera: Piophilidae) in South Korea: confirmation of specimens from human corpses based on cytochrome c oxidase subunit I barcodes. Journal of Forensic Sciences, 66, 1538-1544.
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870-1874.
López-García, J., Angell, C. & and Martín-Vega, D. (2020) Wing morphometrics for the identification of Nearctic and Palaearctic Piophilidae (Diptera) of forensic relevance. Forensic Science International 309, 110192.
Martín-Vega, D. (2011) Skipping clues: forensic importance of the family Piophilidae (Diptera). Forensic Science International, 212, 1-5.
Martín-Vega, D. (2017) Description of the puparium of Parapiophila flavipes (Zetterstedt, 1847)(Diptera; Piophilidae) from specimens in the collection of the Natural History Museum, London. Boletin de la Asociacion Espanola de Entomologia, 41, 239-245.
Martín-Vega, D. & Baz, A. (2014) Comparative larval morphology of the European bone-skippers, Thyreophora cynophila (panzer, 1798) and Centrophlebomyia furcata (Fabricius, 1794)(Diptera: Piophilidae), with notes on their coexistence and natural history. Journal of Natural History, 48, 285-299.
Martín-Vega, D., Baz, A. & Díaz-Aranda, L.M. (2012) The immature stages of the necrophagous fly, Prochyliza nigrimana: comparison with Piophila casei and medicolegal considerations (Diptera: Piophilidae). Parasitology Research, 111, 1127-1135.
Martín-Vega, D., Díaz-Aranda, L.M. & Baz, A. (2014) The immature stages of the necrophagous fly Liopiophila varipes and considerations on the genus Liopiophila (Diptera: Piophilidae). Deutsche Entomologische Zeitschrift, 61, 37-42.
Martín-Vega, D., Hall, M.J.R. & Simonsen, T.J. (2016) Resolving confusion in the use of concepts and terminology in intrapuparial development studies of cyclorrhaphous Diptera. Journal of Medical Entomology, 53, 1249-1251.
Martín-Vega, D., Simonsen, T.J. & Hall, M.J. (2017) Looking into the puparium: micro-CT visualization of the internal morphological changes during metamorphosis of the blow fly, Calliphora vicina, with the first quantitative analysis of organ development in cyclorrhaphous dipterans. Journal of Morphology, 278, 629-651.
Matuszewski, S. & Mądra-Bielewicz, A. (2019) Post-mortem interval estimation based on insect evidence in a quasi-indoor habitat. Science and Justice, 59, 109-115.
McAlpine, J.F. (1977) A revised classification of the Piophilidae, including ‘Neottiophilidae’ and ‘Thyreophoridae’ (Diptera: Schizophora). Memoirs of the Entomological Society of Canada, 109, 1-66.
Mei, M., Whitmore, D., Giudice, G.L. & Cerretti, P. (2013) A neotype designation for the bone-skipper Centrophlebomyia anthropophaga (Diptera, Piophilidae, Thyreophorina), with a review of the Palaearctic species of Centrophlebomyia. ZooKeys, 310, 7-28.
Ozerov, A. (2004) On the classification of the family Piophilidae (Diptera). Zoologicheskii zhurnal, 83, 600-608.
Panagiotakopulu, E. (2004) Dipterous remains and archaeological interpretation. Journal of Archaeological Science, 31, 1675-1684.
Paños, A., Arnaldos, M., García, M. & Ubero-Pascal, N. (2013) Ultrastructure of preimaginal stages of Piophila megastigmata McAlpine, 1978 (Diptera, Piophilidae): a fly of forensic importance. Parasitological Research, 112, 3771-3788.
Pradelli, J., Tuccia, F., Giordani, G. & Vanin, S. (2021) Puparia cleaning techniques for forensic and Archaeo-funerary studies. Insects, 12, 104.
Prado e Castro, C., Cunha, E., Serrano, A. & García, M.D. (2012) Piophila megastigmata (Diptera: Piophilidae): first records on human corpses. Forensic Science International, 214, 23-26.
Rochefort, S., Giroux, M., Savage, J. & Wheeler, T.A. (2015) Key to forensically important Piophilidae (Diptera) in the Nearctic region. Canadian Journal of Arthropod Identification, 27, 1-37.
Rotheray, G.E. & Hancock, E.G. (2012) Puparial morphology and development sites of Neottiophilum praestum, Allopiophila vulgaris, Mycetaulus bipunctatus and Prochyliza varipes (Diptera, Piophilidae). Dipterists Digest, 19, 107-124.
Simmons, P. (1927) The cheese skipper as a pest in cured meats. Washington, D.C. (USA): US Department of Agriculture.
Skidmore, P. (1985) The biology of the Muscidae of the world. Netherlands, Dordrecht: Springer.
Smith, K.G.V. (1986) A manual of forensic entomology. Ithaca: Cornell University Press.
Szpila, K. (2010) Key for the identification of third instars of European blowflies (Diptera: Calliphoridae) of forensic importance. In: Amendt, J., Goff, M.L., Campobasso, P.C. & Grassberger, M. (Eds.) Current concepts in forensic entomology. Netherlands, Dordrecht: Springer, pp. 43-56.
Szpila, K., Richet, R. & Pape, T. (2015) Third instar larvae of flesh flies (Diptera: Sarcophagidae) of forensic importance-critical review of characters and key for European species. Parasitology Research, 114, 2279-2289.
Tamura, K., Nei, M. & Kumar, S. (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences (USA), 101, 11030-11035.
Tuccia, F., Giordani, G., Cattaneo, C., Mazzarelli, D. & Vanin, S. (2021) First record of Physyphora alceae (Preyssler, 1791)(Diptera, Ulidiidae) from a forensic case in northern Italy: description of immature stages, DNA barcoding and phylogenetic analysis. The European Zoological Journal, 88, 1071-1083.
Tuccia, F., Giordani, G. & Vanin, S. (2016) A combined protocol for identification of maggots of forensic interest. Science and Justice, 56, 264-268.
Tuccia, F., Giordani, G. & Vanin, S. (2022) State of the art of the funerary archaeoentomological investigations in Italy. Archaeological and Anthropological Sciences, 14, 70.
Vanin, S. & Huchet, J.B. (2017) Forensic entomology and funerary Archaeoentomology. In: Schotsmans, E.M., Márquez-Grant, N. & Forbes, S.L. (Eds.) Taphonomy of human remains: forensic analysis of the dead and the depositional environment. Manhattan: Wiley, pp. 167-186.
Zajac, B.K., Martin-Vega, D., Feddern, N., Fremdt, H., Prado e Castro, C., Szpila, K. et al. (2016) Molecular identification and phylogenetic analysis of the forensically important family Piophilidae (Diptera) from different European locations. Forensic Science International, 259, 77-84.
Zumpt, F. (1965) Myiasis in Man and Animals in the Old World: A Textbook for Physicians, Veterinarians and Zoologists, Butterworths, London.
Zuska, J. & Laštovka, P. (1969) Species-composition of the dipterous fauna in various types of food-processing plants in Czechoslovakia. Acta Entomologica Bohemoslovaca, 66, 201-221.