The complete ITS2 barcoding region for Strongylus vulgaris and Strongylus edentatus.
Internal transcribed spacer
Metabarcoding
Nemabiome
Parasite
Species determination
Strongyles
Journal
Veterinary research communications
ISSN: 1573-7446
Titre abrégé: Vet Res Commun
Pays: Switzerland
ID NLM: 8100520
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
03
11
2022
accepted:
29
12
2022
medline:
8
9
2023
pubmed:
5
1
2023
entrez:
4
1
2023
Statut:
ppublish
Résumé
Gastrointestinal nematode parasites are of major concern for horses, where Strongylus vulgaris is considered the most pathogenic among the Strongylus species. Diagnosis of S. vulgaris infections can be determined with next generation sequencing techniques, which are inherently dependent on reference sequences. The best marker for parasitic nematodes is internal transcribed spacer 2 (ITS2) and we provide the first complete ITS2 sequences from five morphologically identified S. vulgaris and additional sequences from two S. edentatus. These sequences have high similarity to already published partial sequences and amplicon sequence variants (ASV) based on next generation sequencing (NGS). The ITS2 sequences from S. vulgaris matched available partial ITS2 sequences and the full ASVs, whereas the S. edentatus sequence matched another complete sequence. We also compare Sanger sequencing and NGS methods and conclude that the ITS2 variation is better represented with NGS methods. Based on this, we recommend that further sequencing of morphologically identified specimens of various species should be performed with NGS cover the intraspecific variation in the ITS2.
Identifiants
pubmed: 36598645
doi: 10.1007/s11259-022-10067-w
pii: 10.1007/s11259-022-10067-w
pmc: PMC10485102
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1767-1771Subventions
Organisme : Foundation for Swedish and Norwegian Equine Research
ID : H-15-47-097
Informations de copyright
© 2023. The Author(s).
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