Characterizing the role of Pdgfra in calvarial development.
Pdgfra
calvarial
mesoderm
mouse
neural crest
osteoblast
osteoprogenitor
Journal
Developmental dynamics : an official publication of the American Association of Anatomists
ISSN: 1097-0177
Titre abrégé: Dev Dyn
Pays: United States
ID NLM: 9201927
Informations de publication
Date de publication:
05 2023
05 2023
Historique:
revised:
21
12
2022
received:
05
08
2022
accepted:
28
12
2022
pmc-release:
01
05
2024
medline:
3
5
2023
pubmed:
7
1
2023
entrez:
6
1
2023
Statut:
ppublish
Résumé
Mammalian calvarium is composed of flat bones developed from two origins, neural crest, and mesoderm. Cells from both origins exhibit similar behavior but express distinct transcriptomes. It is intriguing to ask whether genes shared by both origins play similar or distinct roles in development. In the present study, we have examined the role of Pdgfra, which is expressed in both neural crest and mesoderm, in specific lineages during calvarial development. We found that in calvarial progenitor cells, Pdgfra is needed to maintain normal proliferation and migration of neural crest cells but only proliferation of mesoderm cells. Later in calvarial osteoblasts, we found that Pdgfra is necessary for both proliferation and differentiation of neural crest-derived cells, but not for differentiation of mesoderm-derived cells. We also examined the potential interaction between Pdgfra and other signaling pathway involved in calvarial osteoblasts but did not identify significant alteration of Wnt or Hh signaling activity in Pdgfra genetic models. Pdgfra is required for normal calvarial development in both neural crest cells and mesoderm cells, but these lineages exhibit distinct responses to alteration of Pdgfra activity.
Sections du résumé
BACKGROUND
Mammalian calvarium is composed of flat bones developed from two origins, neural crest, and mesoderm. Cells from both origins exhibit similar behavior but express distinct transcriptomes. It is intriguing to ask whether genes shared by both origins play similar or distinct roles in development. In the present study, we have examined the role of Pdgfra, which is expressed in both neural crest and mesoderm, in specific lineages during calvarial development.
RESULTS
We found that in calvarial progenitor cells, Pdgfra is needed to maintain normal proliferation and migration of neural crest cells but only proliferation of mesoderm cells. Later in calvarial osteoblasts, we found that Pdgfra is necessary for both proliferation and differentiation of neural crest-derived cells, but not for differentiation of mesoderm-derived cells. We also examined the potential interaction between Pdgfra and other signaling pathway involved in calvarial osteoblasts but did not identify significant alteration of Wnt or Hh signaling activity in Pdgfra genetic models.
CONCLUSIONS
Pdgfra is required for normal calvarial development in both neural crest cells and mesoderm cells, but these lineages exhibit distinct responses to alteration of Pdgfra activity.
Identifiants
pubmed: 36606407
doi: 10.1002/dvdy.564
pmc: PMC10159935
mid: NIHMS1864297
doi:
Substances chimiques
Receptor Protein-Tyrosine Kinases
EC 2.7.10.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
589-604Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE028918
Pays : United States
Informations de copyright
© 2023 American Association for Anatomy.
Références
Dev Biol. 2012 Aug 15;368(2):283-93
pubmed: 22664175
Dev Cell. 2009 Feb;16(2):303-13
pubmed: 19217431
PLoS One. 2014 Dec 03;9(12):e113785
pubmed: 25470749
Hum Mol Genet. 2010 May 1;19(9):1678-89
pubmed: 20124286
Elife. 2015 May 07;4:
pubmed: 25951516
J Anat. 2005 Nov;207(5):637-53
pubmed: 16313397
Gene Expr Patterns. 2019 Dec;34:119060
pubmed: 31228576
Cell Physiol Biochem. 2017;43(6):2525-2534
pubmed: 29130970
Curr Top Dev Biol. 2015;115:377-410
pubmed: 26589933
PLoS One. 2010 Nov 18;5(11):e14033
pubmed: 21124973
Development. 2015 Apr 1;142(7):1357-67
pubmed: 25742798
Genes Dev. 1996 Feb 1;10(3):301-12
pubmed: 8595881
Hum Mol Genet. 1997;6(10):1647-56
pubmed: 9300656
Mol Cell Biol. 2003 Jun;23(11):4013-25
pubmed: 12748302
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
Cell Adh Migr. 2010 Oct-Dec;4(4):561-6
pubmed: 20657170
Genesis. 2019 Jan;57(1):e23248
pubmed: 30155972
Dev Biol. 2013 Jul 15;379(2):229-34
pubmed: 23648512
Development. 2006 Aug;133(16):3231-44
pubmed: 16854976
Development. 2012 Apr;139(7):1346-58
pubmed: 22395741
PLoS Genet. 2013;9(9):e1003851
pubmed: 24086166
Front Physiol. 2020 Nov 02;11:588901
pubmed: 33224039
Development. 2017 Nov 1;144(21):4026-4036
pubmed: 28947535
Dis Model Mech. 2012 Nov;5(6):948-55
pubmed: 22773757
Curr Top Dev Biol. 2015;115:131-56
pubmed: 26589924
J Bone Miner Res. 2020 Dec;35(12):2458-2469
pubmed: 32777109
Development. 1999 Aug;126(15):3437-47
pubmed: 10393122
Curr Top Dev Biol. 2019;133:281-307
pubmed: 30902256
J Orthop Res. 2011 Dec;29(12):1795-803
pubmed: 21618276
Mol Cell Biol. 2009 Feb;29(3):881-91
pubmed: 19047372
Development. 2003 Dec;130(24):6131-42
pubmed: 14597577
Genes Dev. 2020 Dec 1;34(23-24):1735-1752
pubmed: 33184218
Mech Dev. 2007 Sep-Oct;124(9-10):729-45
pubmed: 17693062
Development. 2006 Sep;133(18):3695-707
pubmed: 16936073
Development. 2003 Feb;130(3):507-18
pubmed: 12490557
Dev Biol. 2020 Oct 1;466(1-2):36-46
pubmed: 32800757
Mech Dev. 2008 Sep-Oct;125(9-10):797-808
pubmed: 18617001
Nat Med. 2014 Sep;20(9):1035-42
pubmed: 25150496
Calcif Tissue Int. 2015 Feb;96(2):129-37
pubmed: 25550101
Dev Biol. 2002 Jan 1;241(1):106-16
pubmed: 11784098
Dev Dyn. 2018 Feb;247(2):304-314
pubmed: 29115005
Genes Dev. 2008 May 15;22(10):1276-312
pubmed: 18483217