Monosynaptic restriction of the anterograde herpes simplex virus strain H129 for neural circuit tracing.
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
04 2023
04 2023
Historique:
revised:
09
11
2022
received:
14
06
2022
accepted:
13
12
2022
pmc-release:
01
04
2024
pubmed:
7
1
2023
medline:
11
2
2023
entrez:
6
1
2023
Statut:
ppublish
Résumé
Identification of synaptic partners is a fundamental task for systems neuroscience. To date, few reliable techniques exist for whole brain labeling of downstream synaptic partners in a cell-type-dependent and monosynaptic manner. Herein, we describe a novel monosynaptic anterograde tracing system based on the deletion of the gene UL6 from the genome of a cre-dependent version of the anterograde Herpes Simplex Virus 1 strain H129. Given that this knockout blocks viral genome packaging and thus viral spread, we reasoned that co-infection of a HSV H129 ΔUL6 virus with a recombinant adeno-associated virus expressing UL6 in a cre-dependent manner would result in monosynaptic spread from target cre-expressing neuronal populations. Application of this system to five nonreciprocal neural circuits resulted in labeling of neurons in expected projection areas. While some caveats may preclude certain applications, this system provides a reliable method to label postsynaptic partners in a brain-wide fashion.
Identifiants
pubmed: 36606699
doi: 10.1002/cne.25451
pmc: PMC10040246
mid: NIHMS1860606
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
584-595Subventions
Organisme : NEI NIH HHS
ID : R01 EY022577
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH097061
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH063912
Pays : United States
Organisme : NIMH NIH HHS
ID : U01 MH114819
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS075531
Pays : United States
Informations de copyright
© 2023 Wiley Periodicals LLC.
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