Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 01 2023
06 01 2023
Historique:
received:
13
10
2022
accepted:
02
01
2023
entrez:
7
1
2023
pubmed:
8
1
2023
medline:
11
1
2023
Statut:
epublish
Résumé
Neural tube closure (NTC) is a complex process of embryonic development involving molecular, cellular, and biomechanical mechanisms. While the genetic factors and biochemical signaling have been extensively investigated, the role of tissue biomechanics remains mostly unexplored due to the lack of tools. Here, we developed an optical modality that can conduct time-lapse mechanical imaging of neural plate tissue as the embryo is experiencing neurulation. This technique is based on the combination of a confocal Brillouin microscope and a modified ex ovo culturing of chick embryo with an on-stage incubator. With this technique, for the first time, we captured the mechanical evolution of the neural plate tissue with live embryos. Specifically, we observed the continuous increase in tissue modulus of the neural plate during NTC for ex ovo cultured embryos, which is consistent with the data of in ovo culture as well as previous studies. Beyond that, we found that the increase in tissue modulus was highly correlated with the tissue thickening and bending. We foresee this non-contact and label-free technique opening new opportunities to understand the biomechanical mechanisms in embryonic development.
Identifiants
pubmed: 36609620
doi: 10.1038/s41598-023-27456-z
pii: 10.1038/s41598-023-27456-z
pmc: PMC9823106
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
263Subventions
Organisme : NICHD NIH HHS
ID : K25 HD097288
Pays : United States
Organisme : NICHD NIH HHS
ID : R01 HD095520
Pays : United States
Informations de copyright
© 2023. The Author(s).
Références
Nat Protoc. 2021 Feb;16(2):1251-1275
pubmed: 33452504
Invest Ophthalmol Vis Sci. 2013 Feb 19;54(2):1418-25
pubmed: 23361513
Lancet Neurol. 2013 Aug;12(8):799-810
pubmed: 23790957
Nat Methods. 2015 Dec;12(12):1132-4
pubmed: 26436482
Nat Rev Genet. 2013 Oct;14(10):733-44
pubmed: 24045690
Opt Lett. 2022 Mar 15;47(6):1347-1350
pubmed: 35290310
Nat Methods. 2019 Oct;16(10):969-977
pubmed: 31548707
J Biomech. 2010 Jan 5;43(1):63-70
pubmed: 19815213
Curr Opin Genet Dev. 2011 Oct;21(5):530-7
pubmed: 21840706
Anat Rec. 1987 Jun;218(2):196-206
pubmed: 3619087
Nat Rev Genet. 2003 Oct;4(10):784-93
pubmed: 13679871
Dev Dyn. 2001 Mar;220(3):284-9
pubmed: 11241836
Birth Defects Res. 2019 Aug 15;111(14):991-998
pubmed: 30239173
Small. 2020 May;16(18):e1907688
pubmed: 32243075
J Biomech. 2005 Oct;38(10):2087-94
pubmed: 16084209
Nat Methods. 2014 Feb;11(2):183-9
pubmed: 24317254
Sci Adv. 2020 Jul 31;6(31):eaba6505
pubmed: 32789173
Mech Dev. 2004 Oct;121(10):1189-97
pubmed: 15327780
J Biomed Opt. 2017 Aug;22(8):1-6
pubmed: 28861955
Birth Defects Res. 2017 Jan 30;109(2):153-168
pubmed: 27620928
Nat Methods. 2018 Aug;15(8):562-563
pubmed: 30065365
Elife. 2022 Jan 10;11:
pubmed: 35001870
Biomed Opt Express. 2019 Feb 25;10(3):1420-1431
pubmed: 30891356
Nat Methods. 2018 Aug;15(8):561-562
pubmed: 30065366
Cell. 2012 May 25;149(5):1084-97
pubmed: 22632972
Dev Growth Differ. 2012 Apr;54(3):306-16
pubmed: 22524603
Dev Biol. 2010 May 1;341(1):5-19
pubmed: 19751720
Development. 2015 Feb 15;142(4):692-701
pubmed: 25670794
Development. 2017 Feb 15;144(4):552-566
pubmed: 28196803
Nature. 2018 Feb 22;554(7693):523-527
pubmed: 29443958
Development. 2009 Feb;136(4):677-88
pubmed: 19168681
Appl Phys Lett. 2018 Apr 16;112(16):163701
pubmed: 29713091
Semin Cell Dev Biol. 2016 Jul;55:119-30
pubmed: 27061360
Biophys J. 2015 Jul 7;109(1):154-63
pubmed: 26153712
Development. 2019 Sep 16;146(18):
pubmed: 31527062
Cell. 1998 Feb 20;92(4):547-57
pubmed: 9491895
Methods. 2016 Feb 1;94:120-8
pubmed: 26255132
Science. 2013 Mar 1;339(6123):1222002
pubmed: 23449594
J Vis Exp. 2016 Dec 28;(118):
pubmed: 28060338
Dev Dyn. 1992 Dec;195(4):231-72
pubmed: 1304821
Acta Biomater. 2019 Oct 1;97:524-534
pubmed: 31377425
Annu Rev Genet. 2014;48:583-611
pubmed: 25292356
Development. 1990 Jun;109(2):243-70
pubmed: 2205465
Proc Natl Acad Sci U S A. 2017 Jun 27;114(26):E5177-E5186
pubmed: 28607062
J Embryol Exp Morphol. 1975 Dec;34(3):559-74
pubmed: 1082486
Dev Dyn. 2001 Jun;221(2):117-45
pubmed: 11376482
Nature. 2011 Aug 03;476(7358):57-62
pubmed: 21814276
Development. 2017 Dec 1;144(23):4249-4260
pubmed: 29183938
Dev Cell. 2002 Jun;2(6):695-706
pubmed: 12062082
Nat Rev Neurosci. 2006 Sep;7(9):724-31
pubmed: 16924261