Antibacterial Thiopeptide GE2270-Congeners from
GE2270
RiPPs
antibacterial
natural products
structure elucidation
thiopeptide
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
23 Dec 2022
23 Dec 2022
Historique:
received:
01
11
2022
revised:
24
11
2022
accepted:
28
11
2022
entrez:
8
1
2023
pubmed:
9
1
2023
medline:
11
1
2023
Statut:
epublish
Résumé
Thiopeptides are macrocyclic natural products with potent bioactivity. Nine new natural thiopeptides (1−9) were obtained from a Nonomuraea jiangxiensis isolated from a terrestrial soil sample collected in Singapore. Even though some of these compounds were previously synthesized or isolated from engineered strains, herein we report the unprecedented isolation of these thiopeptides from a native Nonomuraea jiangxiensis. A comparison with the literature and a detailed analysis of the NMR and HRMS of compounds 1−9 was conducted to assign their chemical structures. The structures of all new compounds were highly related to the thiopeptide antibiotics GE2270, with variations in the substituents on the thiazole and amino acid moieties. Thiopeptides 1−9 exhibited a potent antimicrobial activity against the Gram-positive bacteria, Staphylococcus aureus with MIC90 values ranging from 2 µM to 11 µM. In addition, all compounds were investigated for their cytotoxicity against the human cancer cell line A549, none of the compounds were cytotoxic.
Identifiants
pubmed: 36615295
pii: molecules28010101
doi: 10.3390/molecules28010101
pmc: PMC9822014
pii:
doi:
Substances chimiques
GE 2270 A
JB4J58L2K1
Peptides
0
Thiazoles
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : This work was financially supported by Natural Product Research Laboratory Biomedical Research Council of A*STAR (Agency for Science, Technology and Research) Transition Fund (H16/99/b0/004), National Research Foundation Singapore (NRF-CRP17-2017-06) and
ID : This work was financially supported by Natural Product Research Laboratory Biomedical Research Council of A*STAR (Agency for Science, Technology and Research) Transition Fund (H16/99/b0/004), National Research Foundation Singapore (NRF-CRP17-2017-06) and
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