Liquid storage of Ostrich (Struthio camelus) semen at 5 °C through intermediate dilution.
Dilution temperature
Semen dilution
Semen processing
Semen storage in vitro
Sperm function
Sperm temperature sensitivity
Journal
Animal reproduction science
ISSN: 1873-2232
Titre abrégé: Anim Reprod Sci
Pays: Netherlands
ID NLM: 7807205
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
received:
03
05
2021
revised:
01
11
2022
accepted:
04
11
2022
pubmed:
10
1
2023
medline:
15
3
2023
entrez:
9
1
2023
Statut:
ppublish
Résumé
Dilution rate, dilution temperature and storage time have been recognized as vital steps in the processing of semen for storage before artificial insemination. The objective of this study was to determine optimal dilution and dilution temperature with an ostrich-specific semen extender for chilled storage. Four preselected ostrich (Struthio camelus var. domesticus) males, known for their ease of collection and specific semen quality parameters, were collected using the "dummy" female method. Dilution of 384 semen samples, at rates of 1:1, 1:2, 1:4 and 1:8 semen/diluent ratio with a diluent set at 5, 21 and 38 °C was performed and stored for 48 h at 5 °C. In vitro sperm function tests were conducted to evaluate treated semen during different storage intervals of 1, 5, 24 and 48 h. Motility and kinematic parameters were measured by the Sperm Class Analyzer®, the percentage live sperm measured by fluorescence SYBR14®/PI (LIVE/DEAD®), the percentage of sperm able to resist the hypo-osmotic swelling (HOS) stress test and sperm morphology determined by Nigrosin-Eosin staining. Progressive motility (PMOT), motility (MOT), sperm kinematics, LIVE and HOS were best (P < 0.05) maintained at a higher dilution of 1:4-1:8. The beneficial effect (P < 0.05) of a higher dilution temperature (21 °C) was prominent in terms of PMOT at a higher dilution. Storage of chilled semen at 5 °C requires dilution, at interpolated rates of 1:6-1:7, together with an extender temperature of 21 °C, to maintain optimal sperm function with minimal loss over a 48 h storage period.
Identifiants
pubmed: 36621189
pii: S0378-4320(22)00227-5
doi: 10.1016/j.anireprosci.2022.107148
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
107148Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors wish to state there is no competing interest involved in the study that produced the paper “Liquid storage of Ostrich (Struthio camelus) semen at 5 °C through intermediate dilution” submitted to the journal Animal Reproduction Science. None of us received any material benefit from our involvement in the study.