The rapid detection of respiratory pathogens in critically ill children.
Critical care
Diagnostic techniques
Healthcare-associated pneumonia
Paediatric
Pneumonia
Respiratory system
Journal
Critical care (London, England)
ISSN: 1466-609X
Titre abrégé: Crit Care
Pays: England
ID NLM: 9801902
Informations de publication
Date de publication:
10 01 2023
10 01 2023
Historique:
received:
01
11
2022
accepted:
06
01
2023
entrez:
10
1
2023
pubmed:
11
1
2023
medline:
13
1
2023
Statut:
epublish
Résumé
Respiratory infections are the most common reason for admission to paediatric intensive care units (PICU). Most patients with lower respiratory tract infection (LRTI) receive broad-spectrum antimicrobials, despite low rates of bacterial culture confirmation. Here, we evaluated a molecular diagnostic test for LRTI to inform the better use of antimicrobials. The Rapid Assay for Sick Children with Acute Lung infection Study was a single-centre, prospective, observational cohort study of mechanically ventilated children (> 37/40 weeks corrected gestation to 18 years) with suspected community acquired or ventilator-associated LRTI. We evaluated the use of a 52-pathogen custom TaqMan Array Card (TAC) to identify pathogens in non-bronchoscopic bronchoalveolar lavage (mini-BAL) samples. TAC results were compared to routine microbiology testing. Primary study outcomes were sensitivity and specificity of TAC, and time to result. We enrolled 100 patients, all of whom were tested with TAC and 91 of whom had matching culture samples. TAC had a sensitivity of 89.5% (95% confidence interval (CI TAC is a reliable and rapid adjunct diagnostic approach for LRTI in critically ill children, with the potential to aid early rationalisation of antimicrobial therapy.
Identifiants
pubmed: 36627688
doi: 10.1186/s13054-023-04303-1
pii: 10.1186/s13054-023-04303-1
pmc: PMC9831374
doi:
Types de publication
Observational Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
11Subventions
Organisme : Medical Research Council
ID : MR/V006118/1
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 205214/Z/16/Z
Pays : United Kingdom
Organisme : Addenbrooke's Charitable Trust, Cambridge University Hospitals
ID : 900240
Organisme : Medical Research Council
ID : MR/N029399/1
Pays : United Kingdom
Organisme : Gates Cambridge Trust
ID : OPP1144
Informations de copyright
© 2023. The Author(s).
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