Characterization of microglia behaviour in healthy and pathological conditions with image analysis tools.
classification
image analysis
microglia
morphometry
neuroinflammation
phagocytosis
Journal
Open biology
ISSN: 2046-2441
Titre abrégé: Open Biol
Pays: England
ID NLM: 101580419
Informations de publication
Date de publication:
01 2023
01 2023
Historique:
entrez:
11
1
2023
pubmed:
12
1
2023
medline:
13
1
2023
Statut:
ppublish
Résumé
Microglia are very sensitive to changes in the environment and respond through morphological, functional and metabolic adaptations. To depict the modifications microglia undergo under healthy and pathological conditions, we developed free access image analysis scripts to quantify microglia morphologies and phagocytosis. Neuron-glia cultures, in which microglia express the reporter tdTomato, were exposed to excitotoxicity or excitotoxicity + inflammation and analysed 8 h later. Neuronal death was assessed by SYTOX staining of nucleus debris and phagocytosis was measured through the engulfment of SYTOX
Identifiants
pubmed: 36629019
doi: 10.1098/rsob.220200
pmc: PMC9832574
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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