PDGF inhibits BMP2-induced bone healing.
Journal
NPJ Regenerative medicine
ISSN: 2057-3995
Titre abrégé: NPJ Regen Med
Pays: United States
ID NLM: 101699846
Informations de publication
Date de publication:
11 Jan 2023
11 Jan 2023
Historique:
received:
19
04
2022
accepted:
03
01
2023
entrez:
11
1
2023
pubmed:
12
1
2023
medline:
12
1
2023
Statut:
epublish
Résumé
Bone regeneration depends on a pool of bone/cartilage stem/progenitor cells and signaling mechanisms regulating their differentiation. Using in vitro approach, we have shown that PDGF signaling through PDGFRβ inhibits BMP2-induced osteogenesis, and significantly attenuates expression of BMP2 target genes. We evaluated outcomes of treatment with two anabolic agents, PDGF and BMP2 using different bone healing models. Targeted deletion of PDGFRβ in αSMA osteoprogenitors, led to increased callus bone mass, resulting in improved biomechanical properties of fractures. In critical size bone defects BMP2 treatment increased proportion of osteoprogenitors, while the combined treatment of PDGF BB with BMP2 decreased progenitor number at the injury site. BMP2 treatment induced significant bone formation and increased number of osteoblasts, while in contrast combined treatment with PDGF BB decreased osteoblast numbers. This is in vivo study showing that PDGF inhibits BMP2-induced osteogenesis, but inhibiting PDGF signaling early in healing process does not improve BMP2-induced bone healing.
Identifiants
pubmed: 36631491
doi: 10.1038/s41536-023-00276-5
pii: 10.1038/s41536-023-00276-5
pmc: PMC9834334
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR055607
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR070813
Pays : United States
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
ID : AR055607
Organisme : U.S. Department of Health & Human Services | NIH | National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
ID : AR070813
Informations de copyright
© 2023. The Author(s).
Références
Curr Osteoporos Rep. 2020 Dec;18(6):655-665
pubmed: 33034805
Elife. 2021 Feb 09;10:
pubmed: 33560227
Am J Physiol. 1997 May;272(5 Pt 1):C1709-16
pubmed: 9176163
Spine (Phila Pa 1976). 2002 Aug 15;27(16 Suppl 1):S40-8
pubmed: 12205419
J Bone Miner Res. 2014;29(5):1283-94
pubmed: 24190076
Tissue Eng Part B Rev. 2016 Aug;22(4):284-97
pubmed: 26857241
J Periodontol. 2005 Dec;76(12):2205-15
pubmed: 16332231
Int J Oral Maxillofac Implants. 2015 Jan-Feb;30(1):196-201
pubmed: 25265123
J Biol Chem. 1994 Dec 2;269(48):30546-52
pubmed: 7982973
J Periodontol. 2013 Apr;84(4):456-64
pubmed: 22612364
J Orthop Res. 2017 Feb;35(2):213-223
pubmed: 27743449
JBMR Plus. 2019 Jan 15;3(5):e10127
pubmed: 31131345
Endocrinology. 1994 Mar;134(3):1423-8
pubmed: 8119182
PLoS One. 2014 Jan 28;9(1):e86757
pubmed: 24489784
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
J Bone Miner Res. 2002 Jan;17(1):15-25
pubmed: 11771662
Mater Sci Eng C Mater Biol Appl. 2021 Nov;130:112466
pubmed: 34702541
NPJ Regen Med. 2017 Dec 15;2:32
pubmed: 29302365
J Bone Miner Res. 2000 Apr;15(4):663-73
pubmed: 10780858
Dev Cell. 2019 Oct 21;51(2):236-254.e12
pubmed: 31543445
J Bone Miner Res. 2009 Feb;24(2):274-82
pubmed: 18847330
J Orthop Res. 2014 Sep;32(9):1120-8
pubmed: 24888702
Nat Med. 2014 Nov;20(11):1270-8
pubmed: 25282358
J Appl Biomater Funct Mater. 2021 Jan-Dec;19:2280800020987405
pubmed: 33541198
Bone. 1994 Mar-Apr;15(2):203-8
pubmed: 8086239
J Orthop Res. 2020 Nov;38(11):2350-2361
pubmed: 32141629
J Bone Miner Res. 2008 Sep;23(9):1519-28
pubmed: 18410236
Physiol Rev. 1999 Oct;79(4):1283-316
pubmed: 10508235
Invest Ophthalmol Vis Sci. 2010 Feb;51(2):1002-7
pubmed: 19797221
J Clin Invest. 1998 Dec 15;102(12):2106-14
pubmed: 9854046
Stem Cells. 2020 Apr;38(4):530-541
pubmed: 31859429
PLoS One. 2012;7(2):e30503
pubmed: 22355314
J Bone Miner Res. 2020 Dec;35(12):2458-2469
pubmed: 32777109
J Orthop Res. 2008 Jan;26(1):83-90
pubmed: 17676626
FASEB J. 2002 Oct;16(12):1575-83
pubmed: 12374780
Spine (Phila Pa 1976). 2002 Nov 1;27(21):2396-408
pubmed: 12438990
Nat Rev Endocrinol. 2016 Apr;12(4):203-21
pubmed: 26893264
Genes Dev. 2008 Dec 1;22(23):3255-67
pubmed: 19056881
Plast Reconstr Surg Glob Open. 2019 Aug 19;7(8):e2347
pubmed: 31592029
J Vis Exp. 2016 Sep 14;(115):
pubmed: 27684089
J Bone Miner Res. 2007 Nov;22(11):1679-89
pubmed: 17663639
J Spinal Disord Tech. 2002 Oct;15(5):337-49
pubmed: 12394656
J Periodontol. 2003 Sep;74(9):1282-92
pubmed: 14584860
J Mol Cell Cardiol. 1997 Jan;29(1):357-68
pubmed: 9040050
J Periodontol. 1991 Jul;62(7):458-67
pubmed: 1920013
Stem Cells. 2012 Feb;30(2):187-96
pubmed: 22083974