Impact of PIEZO1-channel on inflammation and osteoclastogenesis mediated via periodontal ligament fibroblasts during mechanical loading.
PIEZO1
mechanosensitive electron channels
orthodontic tooth movement
periodontal ligament fibroblasts
Journal
European journal of oral sciences
ISSN: 1600-0722
Titre abrégé: Eur J Oral Sci
Pays: England
ID NLM: 9504563
Informations de publication
Date de publication:
02 2023
02 2023
Historique:
received:
22
11
2022
accepted:
16
12
2022
pubmed:
13
1
2023
medline:
31
1
2023
entrez:
12
1
2023
Statut:
ppublish
Résumé
The identification of mechanosensitive ion channels and their importance in innate immunity provides new starting points to elucidate the molecular mechanisms of orthodontic tooth movement. The mechanosensitive electron channel PIEZO1 (Piezo Type Mechanosensitive Ion Channel Component 1) may play a crucial role in orthodontic tooth movement. To investigate the role of the PIEZO1 channel, periodontal ligament fibroblasts (PDLF) were subsequently treated with a PIEZO1 inhibitor (GsMTx) with simultaneous pressure application or with an activator (JEDI2) without mechanical strain. The expression of genes and proteins involved in orthodontic tooth movement was examined by RT-qPCR, Western blot and ELISA. In addition, the effect on PDLF-mediated osteoclastogenesis was investigated in a coculture model using human monocytes. Inhibition of PIEZO1 under pressure application caused a reduction in RANKL (receptor activator of NF-kB ligand) expression, resulting in decreased osteoclastogenesis. On the other hand, activation of PIEZO1 without mechanical strain downregulated OPG (osteoprotegerin), resulting in increased osteoclastogenesis. PIEZO1 appears to play a role in the induction of inflammatory genes. It was also shown to influence osteoclastogenesis.
Substances chimiques
PIEZO1 protein, human
0
Ion Channels
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e12913Informations de copyright
© 2023 The Authors. European Journal of Oral Sciences published by John Wiley & Sons Ltd on behalf of Scandinavian Division of the International Association for Dental Research.
Références
Sá-Pinto AC. Association between malocclusion and dental caries in adolescents: a systematic review and meta-analysis. Eur Arch Paediatr Dent. 2018;19:73-82. https://doi.org/10.1007/s40368-018-0333-0
Staufer K, Landmesser H. Effects of crowding in the lower anterior segment-a risk evaluation depending upon the degree of crowding. J Orofac Orthop. 2004;65:13-25. https://doi.org/10.1007/s00056-004-0207-4
Alsulaiman AA, Kaye E, Jones J, Cabral H, Leone C, Will L, et al. Incisor malalignment and the risk of periodontal disease progression. Am J Orthod Dentofacial Orthop. 2018;153:512-22. https://doi.org/10.1016/j.ajodo.2017.08.015
Meikle MC. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. Eur J Orthod. 2006;28:221-40. https://doi.org/10.1093/ejo/cjl001
Lekic P, McCulloch C. Periodontal ligament cell populations: the central role of fibroblasts in creating a unique tissue. Anat Rec. 1996;245:327-41. https://doi.org/10.1002/(SICI)1097-0185(199606)245:2<327:AID-AR15>3.0.CO;2-R
Nanci A, Bosshardt DD. Structure of periodontal tissues in health and disease. Periodontol 2000. 2006;40:11-28. https://doi.org/10.1111/j.1600-0757.2005.00141.x
Kanzaki H, Chiba M, Shimizu Y, Mitani H. Periodontal ligament cells under mechanical stress induce osteoclastogenesis by receptor activator of nuclear factor kappaB ligand up-regulation via prostaglandin E2 synthesis. J Bone Miner Res. 2002;17:210-20. https://doi.org/10.1359/jbmr.2002.17.2.210
Yamaguchi M, Kasai K. Inflammation in periodontal tissues in response to mechanical forces. Arch Immunol Ther Exp (Warsz). 2005;53:388-98.
Li Y, Jacox LA, Little SH, Ko C-C. Orthodontic tooth movement: the biology and clinical implications. Kaohsiung J Med Sci. 2018;34:207-14. https://doi.org/10.1016/j.kjms.2018.01.007
Yamaguchi M. RANK/RANKL/OPG during orthodontic tooth movement. Orthod Craniofac Res. 2009;12:113-19. https://doi.org/10.1111/j.1601-6343.2009.01444.x
Jin Y, Li J, Wang Y, Ye R, Feng X, Jing Z, Zhao Z. Functional role of mechanosensitive ion channel Piezo1 in human periodontal ligament cells. Angle Orthod. 2015;85:87-4. https://doi.org/10.2319/123113-955.1
Bae C, Sachs F, Gottlieb PA. The mechanosensitive ion channel Piezo1 is inhibited by the peptide GsMTx4. Biochemistry. 2011;50:6295-300. https://doi.org/10.1021/bi200770q
Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, et al. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science. 2010;330:55-60. https://doi.org/10.1126/science.1193270
Zhao Q, Zhou H, Li X, Xiao B. The mechanosensitive Piezo1 channel: a three-bladed propeller-like structure and a lever-like mechanogating mechanism. FEBS J. 2019;286:2461-70. https://doi.org/10.1111/febs.14711
Wang Y, Chi S, Guo H, Li G, Wang L, Zhao Q, et al. A lever-like transduction pathway for long-distance chemical- and mechano-gating of the mechanosensitive Piezo1 channel. Nat Commun. 2018;9:1300. https://doi.org/10.1038/s41467-018-03570-9
Wang L, Zhou H, Zhang M, Liu W, Deng T, Zhao Q, et al. Structure and mechanogating of the mammalian tactile channel PIEZO2. Nature. 2019;573:225-9. https://doi.org/10.1038/s41586-019-1505-8
Wang L, You X, Zhang L, Zhang C, Zou W. Mechanical regulation of bone remodeling. Bone Res. 2022;10:16. https://doi.org/10.1038/s41413-022-00190-4
Sun W, Chi S, Li Y, Ling S, Tan Y, Xu Y, et al. T. Elife. 2019;8:e47454. https://doi.org/10.7554/eLife.474
Wang L, You X, Lotinun S, Zhang L, Wu N, Zou W. Mechanical sensing protein PIEZO1 regulates bone homeostasis via osteoblast-osteoclast crosstalk. Nat Commun. 2020;11:282. https://doi.org/10.1038/s41467-019-14146-6
Kirschneck C, Bauer M, Gubernator J, Proff P, Schröder A. Comparative assessment of mouse models for experimental orthodontic tooth movement. Sci Rep. 2020;10:12154. https://doi.org/10.1038/s41598-020-69030-x
Schröder A, Bauer K, Spanier G, Proff P, Wolf M, Kirschneck C. Expression kinetics of human periodontal ligament fibroblasts in the early phases of orthodontic tooth movement. J Orofac Orthop. 2018;79:337-51. https://doi.org/10.1007/s00056-018-0145-1
Suchyna TM, Johnson JH, Hamer K, Leykam JF, Gage DA, Clemo HF, et al. Identification of a peptide toxin from Grammostola spatulata spider venom that blocks cation-selective stretch-activated channels. J Gen Physiol. 2000;115:583-98. https://doi.org/10.1085/jgp.115.5.583
Schröder A, Schöniger R, Oeldemann J, Spanier G, Proff P, Jantsch J, et al. An evaluation of different 3D cultivation models on expression profiles of human periodontal ligament fibroblasts with compressive strain. Int J Mol Sci. 2022;23:2029. https://doi.org/10.3390/ijms23042029
Kirschneck C, Batschkus S, Proff P, Köstler J, Spanier G, Schröder A. Valid gene expression normalization by RT-qPCR in studies on hPDL fibroblasts with focus on orthodontic tooth movement and periodontitis. Sci Rep. 2017;7:14751. https://doi.org/10.1038/s41598-017-15281-0
Maneshi MM, Ziegler L, Sachs F, Hua SZ, Gottlieb PA. Enantiomeric Aβ peptides inhibit the fluid shear stress response of PIEZO1. Sci Rep. 2018;8:14267. https://doi.org/10.1038/s41598-018-32572-2
Alcaino C, Knutson K, Gottlieb PA, Farrugia G, Beyder A. Mechanosensitive ion channel Piezo2 is inhibited by D-GsMTx4. Channels (Austin). 2017;11:245-53. https://doi.org/10.1080/19336950.2017.1279370
Shen X, Wu W, Ying Y, Zhou L, Zhu H. A regulatory role of Piezo1 in apoptosis of periodontal tissue and periodontal ligament fibroblasts during orthodontic tooth movement. Aust Endod J. 2022. https://doi.org/10.1111/aej.12721
Wang L, Wang X, Ji N, Li H-M, Cai S-X. Mechanisms of the mechanically activated ion channel Piezo1 protein in mediating osteogenic differentiation of periodontal ligament stem cells via the Notch signaling pathway. Hua Xi Kou Qiang Yi Xue Za Zhi. 2020;38:628-36. https://doi.org/10.7518/hxkq.2020.06.004
Kopan R, Ilagan MXG. The canonical Notch signaling pathway: unfolding the activation mechanism. Cell. 2009;137:216-33. https://doi.org/10.1016/j.cell.2009.03.045