Polycomb repressive complexes 1 and 2 are each essential for maintenance of X inactivation in extra-embryonic lineages.
Journal
Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575
Informations de publication
Date de publication:
Jan 2023
Jan 2023
Historique:
received:
28
04
2021
accepted:
08
11
2022
pubmed:
13
1
2023
medline:
25
1
2023
entrez:
12
1
2023
Statut:
ppublish
Résumé
In female mammals, one of the two X chromosomes becomes inactivated during development by X-chromosome inactivation (XCI). Although Polycomb repressive complex (PRC) 1 and PRC2 have both been implicated in gene silencing, their exact roles in XCI during in vivo development have remained elusive. To this end, we have studied mouse embryos lacking either PRC1 or PRC2. Here we demonstrate that the loss of either PRC has a substantial impact on maintenance of gene silencing on the inactive X chromosome (Xi) in extra-embryonic tissues, with overlapping yet different genes affected, indicating potentially independent roles of the two complexes. Importantly, a lack of PRC1 does not affect PRC2/H3K27me3 accumulation and a lack of PRC2 does not impact PRC1/H2AK119ub1 accumulation on the Xi. Thus PRC1 and PRC2 contribute independently to the maintenance of XCI in early post-implantation extra-embryonic lineages, revealing that both Polycomb complexes can be directly involved and differently deployed in XCI.
Identifiants
pubmed: 36635505
doi: 10.1038/s41556-022-01047-y
pii: 10.1038/s41556-022-01047-y
doi:
Substances chimiques
Polycomb Repressive Complex 1
EC 2.3.2.27
Polycomb Repressive Complex 2
EC 2.1.1.43
Polycomb-Group Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
134-144Subventions
Organisme : MEXT | JST | Precursory Research for Embryonic Science and Technology (PRESTO)
ID : JPMJPR11SE
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : 17H06426
Organisme : MEXT | JST | Core Research for Evolutional Science and Technology (CREST)
ID : 13417643
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : ANR-11-LABX-0044
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature Limited.
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