Comparative analysis of glass and Hemotek membrane feeding systems for malaria transmission research.
Plasmodium falciparum
artificial feeding system
blood meal
gametocytes
Journal
Transactions of the Royal Society of Tropical Medicine and Hygiene
ISSN: 1878-3503
Titre abrégé: Trans R Soc Trop Med Hyg
Pays: England
ID NLM: 7506129
Informations de publication
Date de publication:
02 06 2023
02 06 2023
Historique:
received:
14
10
2022
revised:
28
12
2022
accepted:
29
12
2022
medline:
5
6
2023
pubmed:
14
1
2023
entrez:
13
1
2023
Statut:
ppublish
Résumé
Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system. Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts. While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane. Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.
Sections du résumé
BACKGROUND
Glass membrane feeders are used in malaria research for artificial blood feeding. This study investigates the use of Hemotek membrane feeders as a standardized alternative feeding system.
METHODS
Hemotek feeders were compared with glass feeders by assessing mosquito feeding rate, imbibed blood meal volume and Plasmodium falciparum infection intensity on mosquito guts.
RESULTS
While mosquito feeding rate and blood meal volume were comparable between Hemotek and glass feeders, a loss in transmission was observed using the Hemotek feeder with a conventional collagen membrane. There was no difference in transmission between both feeders when Parafilm was used as the membrane.
CONCLUSIONS
Hemotek feeders with a Parafilm membrane can be used as an alternative feeding system for malaria transmission research.
Identifiants
pubmed: 36637101
pii: 6986841
doi: 10.1093/trstmh/trac135
pmc: PMC10235806
doi:
Substances chimiques
Paraffin
8002-74-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
476-478Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene.
Références
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pubmed: 32771047
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pubmed: 31693672
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pubmed: 26906747