Anti-Inflammatory Role of TRPV4 in Human Macrophages.


Journal

ImmunoHorizons
ISSN: 2573-7732
Titre abrégé: Immunohorizons
Pays: United States
ID NLM: 101708159

Informations de publication

Date de publication:
01 01 2023
Historique:
received: 16 12 2022
accepted: 16 12 2022
entrez: 16 1 2023
pubmed: 17 1 2023
medline: 19 1 2023
Statut: ppublish

Résumé

The pathology of skin immune diseases such as atopic dermatitis is closely related to the overproduction of cytokines by macrophages. Although the pathological functions of macrophages in skin are known, mechanisms of how they detect the tissue environment remain unknown. TRPV4, a nonselective cation channel with high Ca2+ permeability, is activated at physiological temperatures from 27 to 35°C and involved in the functional control of macrophages. However, the relationship between TRPV4 function in macrophages and skin immune disease is unclear. In this study, we demonstrate that TRPV4 activation inhibits NF-κB signaling, resulting in the suppression of IL-1β production in both human primary monocytes and macrophages derived from human primary monocytes. A TRPV4 activator also inhibited the differentiation of human primary monocytes into GM-CSF M1 macrophages but not M-CSF M2 macrophages. We also observed a significant increase in the number of inducible NO synthase-positive/TRPV4-negative dermal macrophages in atopic dermatitis compared with healthy human skin specimens. Our findings provide insight into the physiological relevance of TRPV4 to the regulation of macrophages during homeostasis maintenance and raise the potential for TRPV4 to be an anti-inflammatory target.

Identifiants

pubmed: 36645854
pii: 237687
doi: 10.4049/immunohorizons.2200100
pmc: PMC10563396
doi:

Substances chimiques

TRPV Cation Channels 0
Cytokines 0
Anti-Inflammatory Agents 0
TRPV4 protein, human 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

81-96

Informations de copyright

Copyright © 2023 The Authors.

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Auteurs

Yukiko Atsumi (Y)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.

Manami Toriyama (M)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara, Japan.

Hiroko Kato (H)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.

Motoki Nakamura (M)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Department of Geriatric and Environmental Dermatology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.

Akimichi Morita (A)

Department of Geriatric and Environmental Dermatology, Graduate School of Medical Sciences, Nagoya City University, Nagoya, Japan.

Masayuki Takaishi (M)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Mandom Corporation, Osaka, Japan.
Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan.

Kaori Saito (K)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
Mandom Corporation, Osaka, Japan.

Miku Tanaka (M)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Mandom Corporation, Osaka, Japan.

Fumihiro Okada (F)

Mandom Corporation, Osaka, Japan.

Makoto Tominaga (M)

Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan.
National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Japan.

Ken J Ishii (KJ)

Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
Laboratory of Vaccine Science, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan; and.
Division of Vaccine Science, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Fumitaka Fujita (F)

Graduate School of Pharmaceutical Sciences, Osaka University, Osaka, Japan.
Center for Vaccine and Adjuvant Research, National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan.
Mandom Corporation, Osaka, Japan.
Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences, Okazaki, Japan.

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