Isolation and Cultivation of Adipose-Derived Mesenchymal Stem Cells Originating from the Infrapatellar Fat Pad Differentiated with Blood Products: Method and Protocol.
adipose-tissue
blood products
infra-patellar-fat pad
joint preservation
mesenchymal-stem-cells
regenerative orthopedics
Journal
Methods and protocols
ISSN: 2409-9279
Titre abrégé: Methods Protoc
Pays: Switzerland
ID NLM: 101720073
Informations de publication
Date de publication:
25 Dec 2022
25 Dec 2022
Historique:
received:
16
11
2022
revised:
20
12
2022
accepted:
23
12
2022
entrez:
17
1
2023
pubmed:
18
1
2023
medline:
18
1
2023
Statut:
epublish
Résumé
Adipose-derived mesenchymal stem cells (ASCs) are a promising source for clinical application in regenerative orthopedics. ASCs derived from the infra-patellar fat pad (IFP)-a distinct adipose structure in the knee-show superior regenerative potential compared to subcutaneous-fat-derived cells. Furthermore, it has been shown that blood products enhance ASCs' viability. A major challenge for clinical translation of both ASCs and blood products is the low comparability of obtained data due to non-standardized harvesting, isolation and preparation methods. The aim of this method-paper is to provide reproducible protocols to help standardize basic research in the field to build a sound basis for clinical translation with an emphasize on practicability. The presented protocols include (i) ASC isolation from the IFP, (ii) blood product preparation and (iii) ASC incubation with blood products.
Identifiants
pubmed: 36648952
pii: mps6010003
doi: 10.3390/mps6010003
pmc: PMC9844469
pii:
doi:
Types de publication
Journal Article
Langues
eng
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